| 1. |
- Hagenbucher, Steffen, et al.
(författare)
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Domestication influences choice behavior and performance of a generalist herbivore
- 2016
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Ingår i: Perspectives in Plant Ecology, Evolution and Systematics. - : Elsevier BV. - 1433-8319 .- 1618-0437. ; 23, s. 63-72
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Tidskriftsartikel (refereegranskat)abstract
- Domestication processes have changed wild ancestral plants to modern crop plants that are well adapted to agronomical practices and produce high yields. However, a major concern with domestication is an increased susceptibility to herbivores and pathogens. Such changes in plant resistance can, directly and indirectly, affect the host choice decisions of herbivorous insects. In this study we investigated how changes in resistance traits during the domestication of cotton, influenced the preference and performance of the generalist moth Spodoptera littoralis. We studied the development of larvae, as well as the choice behavior of larvae and ovipositing females, on a selected group of cotton accessions from three different species: Gossypium hirsutum; Gossypium herbaceum and Gossypium raimondii. Combining preference and performance data would allow us to explain host-plant choice using the preference performance hypotheses. Additionally, we analyzed the volatile profile of the different plants to detect differences that could have played a role in the host-choice of S. littoralis.We could show that domesticated G. hirsutum cotton plants are better host plants for the moth S. littoralis as they supported a better performance than wild plants. Furthermore, we found qualitative and quantitative differences in the volatile bouquet of the different Gossypium types. However, these differences were not clearly represented in the behavior of S. littoralis. As neither larvae nor ovipositing females consistently preferred better over inferior host plants with the exception of G. raimondii that was rejected over more domesticated ones. Gossypium raimondii also provided associational resistance to neighboring susceptible plants. Our findings indicate that domestication in cotton has effects on host plant choice in the generalist herbivore S. littoralis, but that there is no clear pattern of how preference and performance is affected. (C) 2016 Elsevier GmbH. All rights reserved.
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| 2. |
- Roy, Amit, et al.
(författare)
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Data set for diet specific differential gene expression analysis in three Spodoptera moths
- 2016
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Ingår i: Data in Brief. - : Elsevier BV. - 2352-3409. ; 8, s. 448-455
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Tidskriftsartikel (refereegranskat)abstract
- Examination of closely related species pairs is suggested for evolutionary comparisons of different degrees of polyphagy, which we did here with three taxa of lepidopteran herbivores,Spodopteraspp (S. littoralis,S. frugiperdamaize (C) and rice (R) strains) for a RNAseq analysis of the midguts from the 3rd instar insect larvae for differential metabolic responses after feeding on pinto bean based artificial diet vs maize leaves. Paired-end (2×100bp) Illumina HiSeq2500 sequencing resulted in a total of 24, 23, 24, and 21 million reads for the SF-C-Maize, SF-C-Pinto, SF-R-Maize, SF-R Pinto, and a total of 35 and 36 million reads for the SL-Maize and SL-Pinto samples, respectively. After quality control measures, a total of 62.2 million reads from SL and 71.7 million reads from SF were used for transcriptome assembly (TA). The resulting finalde novoreference TA (backbone) for the SF taxa contained 37,985 contigs with a N50 contig size of 1030bp and a maximum contig length of 17,093bp, while for SL, 28,329 contigs were generated with a N50 contig size of 1980bp and a maximum contig length of 18,267bp. The data presented herein contains supporting information related to our research article Roy et al. (2016)http://dx.doi.org/10.1016/j.ibmb.2016.02.006
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| 3. |
- Roy, Amit, et al.
(författare)
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Diet dependent metabolic responses in three generalist insect herbivores Spodoptera spp
- 2016
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Ingår i: Insect Biochemistry and Molecular Biology. - : Elsevier BV. - 0965-1748 .- 1879-0240. ; 71, s. 91-105
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Tidskriftsartikel (refereegranskat)abstract
- Adaption to dietary changes is critical in the evolution of host plant ranges in polyphagous insects. We compared three taxa of lepidopteran herbivores from the predominantly generalist genus Spodoptera showing different degrees of polyphagy: Spodoptera littoralis, with a broad host range including both mono-and dicotyledonous plants, and two Spodoptera frugiperda strains [Corn (i.e. maize) (C) and Rice (R)] adapted primarily to different grass species. When feeding on maize we show a lower performance in the broad generalist taxon compared to the grass adapted taxa. Among these taxa, the maize adapted S. frugiperda C-strain generally performed better than the R-strain on maize leaves. On artificial pinto diet, all taxa performed well. Our RNA-Seq analysis of midgut transcriptomes from 3rd instar larvae feeding on maize showed broader transcriptional readjustments in the generalist S. littoralis compared to grass adapted S. frugiperda strains. Substantial alteration in the expression levels of midgut physiological function related transcripts, such as digestive and detoxifying enzymes, transporters, immunity, and peritrophic membrane associated transcripts, existed in all taxa. We found high background expression of UDP-glucosyl transferases, which are known to neutralize maize leaf toxins, in the maize adapted S. frugiperda C-strain, contributing to its fitness on maize compared to the R-strain. Our findings provide evidence for divergent diet specific response of digestive physiology within these Spodoptera taxa. Unexpectedly, the C- and R-strains of S. frugiperda fed on the same diet showed large differences in expression patterns between these two closely related taxa´.
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