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| 2. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 3. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 4. |
- Kanoni, Stavroula, et al.
(författare)
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Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis.
- 2022
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Ingår i: Genome biology. - : Springer Science and Business Media LLC. - 1474-760X .- 1465-6906 .- 1474-7596. ; 23:1
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Tidskriftsartikel (refereegranskat)abstract
- Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery.To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (N=1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3-5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism.Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk.
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| 5. |
- Kristan, Matej, et al.
(författare)
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The first visual object tracking segmentation VOTS2023 challenge results
- 2023
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Ingår i: 2023 IEEE/CVF International conference on computer vision workshops (ICCVW). - : Institute of Electrical and Electronics Engineers Inc.. - 9798350307443 - 9798350307450 ; , s. 1788-1810
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking Segmentation VOTS2023 challenge is the eleventh annual tracker benchmarking activity of the VOT initiative. This challenge is the first to merge short-term and long-term as well as single-target and multiple-target tracking with segmentation masks as the only target location specification. A new dataset was created; the ground truth has been withheld to prevent overfitting. New performance measures and evaluation protocols have been created along with a new toolkit and an evaluation server. Results of the presented 47 trackers indicate that modern tracking frameworks are well-suited to deal with convergence of short-term and long-term tracking and that multiple and single target tracking can be considered a single problem. A leaderboard, with participating trackers details, the source code, the datasets, and the evaluation kit are publicly available at the challenge website1
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| 6. |
- Chen, Fu qiang, et al.
(författare)
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Turbulent compressible flow analysis on multi-stage high pressure reducing valve
- 2018
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Ingår i: Flow Measurement and Instrumentation. - : Elsevier BV. - 0955-5986. ; 61, s. 26-37
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Tidskriftsartikel (refereegranskat)abstract
- Pressure reducing valve plays an important role in thermodynamic systems. Under extreme operating conditions, greater demands are requested on pressure reducing systems. In this paper, a novel multi-stage high pressure reducing valve (MSHPRV) is proposed, which can achieve multi-stage pressure reducing processes, improve the flow characteristics and deal with complex conditions. Here, the effects of different structural parameters on turbulent compressible flow inside MSHPRV are numerically investigated to achieve low valve noise and energy consumption. Mach number is taken as the parameter to reflect the fluid compressibility. Higher Mach number can cause serious aerodynamic noise and large amount of energy consumption. Based on this, transmission loss of MSHPRV is also studied to achieve better noise control performances. Meanwhile, larger turbulent dissipation rate means larger degree of energy consumption, so it is with the exergy loss. Thus, numerical models with different valve openings, perforated plate diameters, chamfer radii of perforated plates, pressure ratios and stages of perforated plates are established, and the effects of these structural parameters on the compressible turbulent flow and energy consumption of MSHPRV are investigated. Results show that different structural parameters have significant impacts on compressible turbulent flow and energy consumption performance in MSHPRV. The best noise control and least energy consumption of MSHPRV is achieved with such parameters as pressure ratio 7, perforated plate diameter 4 mm and 4 stage plates. This work can benefit the further research work on energy saving and multi-stage design of pressure reducing devices.
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| 7. |
- Xia, Xinxin, et al.
(författare)
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Revealing the crystalline packing structure of Y6 in the active layer of organic solar cells: the critical role of solvent additives
- 2023
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Ingår i: Journal of Materials Chemistry A. - : ROYAL SOC CHEMISTRY. - 2050-7488 .- 2050-7496. ; 11:40, s. 21895-21907
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Tidskriftsartikel (refereegranskat)abstract
- The bulk heterojunction (BHJ) morphology of photovoltaic materials is crucial to the fundamental optoelectronic properties of organic solar cells (OSCs). However, in the photoactive layer, the intrinsic crystalline packing structure of Y6, currently the hallmark molecule among Y-series non-fullerene acceptors (NFAs), has not been unambiguously determined. Here, employing grazing-incidence wide-angle X-ray scattering (GIWAXS), we managed to uncover the intrinsic crystalline packing structure of Y6 in the BHJ active layer of OSCs, which is found to be different from its single-crystal structure reported previously. Moreover, we find that solvent additive 1-chloronaphthalene (CN) can induce highly ordered packing of Y6 in BHJ thin films. With the help of atomistic molecular dynamics simulations, it is revealed that pi-pi interactions generally exist between naphthalene derivatives and IC terminals of Y6 analogues, which would essentially improve their long-range ordering. Our work reveals the intrinsic crystalline packing structure of Y6 in the BHJ active layer as well as its crystallization mechanism in thin films, thus providing direct correlations between this crystalline packing and the device characteristics and photophysical properties.
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| 8. |
- Chen, Min Rui, et al.
(författare)
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Effects of nanoparticles on hydraulic cavitation
- 2018
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Ingår i: XI International Conference on Computational Heat, Mass and Momentum Transfer (ICCHMT 2018). - : EDP Sciences. ; 240
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Konferensbidrag (refereegranskat)abstract
- When liquids flowing through a throttling element, such as a perforated plate, the velocity increases and the pressure decreases. If the pressure is below the saturated vapor pressure, the liquid will vaporize into small bubbles, which is called hydraulic cavitation. In fact, vaporization nucleus is another crucial condition for vaporizing. The nanoparticles contained in the nanofluids play a significant role in vaporization of liquids. In this paper, the effects of the nanoparticles on hydraulic cavitation are investigated. Firstly, a geometric model of a pipe channel equipped with a perforated plate is established. Then with different nanoparticle volume fractions and diameters, the nanofluids flowing through the channel is numerically simulated based on a validated numerical method. The operation conditions, such as the temperature and the pressure ratio of inlet to outlet, are the considered variables. As a significant parameter, cavitation numbers under different operation conditions are achieved to investigate the effects of nanoparticles on hydraulic cavitation. Meanwhile, the contours are extracted to research the distribution of bubbles for further investigation. This study is of interests for researchers working on hydraulic cavitation or nanofluids.
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| 9. |
- Chen, Min Rui, et al.
(författare)
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The hydraulic cavitation affected by nanoparticles in nanofluids
- 2018
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Ingår i: Computation. - : MDPI AG. - 2079-3197. ; 6:3
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Tidskriftsartikel (refereegranskat)abstract
- When liquids flow through a throttling element, the velocity increases and the pressure decreases. At this point, if the pressure is below the saturated vapor pressure of this liquid, the liquid will vaporize into small bubbles, causing hydraulic cavitation. In fact, a vaporization nucleus is another crucial condition for vaporizing, and particles contained in the liquid can also work as the vaporization nuclear. As a novel heat transfer medium, nanofluids have attracted the attention of many scholars. The nanoparticles contained in the nanofluids play a significant role in the vaporization of liquids. In this paper, the effects of the nanoparticles on hydraulic cavitation are investigated. Firstly, a geometric model of a perforated plate, the throttling element in this paper, is established. Then with different nanoparticle volume fractions and diameters, the nanofluids flowing through the perforated plate are numerically simulated based on a validated numerical method. The operation conditions, such as the ratio of inlet to outlet pressures and the temperature are the considered variables. Additionally, cavitation numbers under different operating conditions are achieved to investigate the effects of nanoparticles on hydraulic cavitation. Meanwhile, the contours are extracted to research the distribution of bubbles for further investigation. This study is of interest for researchers working on hydraulic cavitation or nanofluids.
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| 10. |
- Chen, Zhi, et al.
(författare)
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Large-Area Crystalline Zeolitic Imidazolate Framework Thin Films
- 2021
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Ingår i: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 60:25, s. 14124-14130
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Tidskriftsartikel (refereegranskat)abstract
- We report that continuous MOF films with highly controlled thickness (from 44 to 5100 nm) can be deposited over length scales greater than 80 centimeters by a facile, fast, and cost-effective spray-coating method. Such success relies on our discovery of unprecedented perfectly dispersed colloidal solutions consisting of amorphous MOF nanoparticles, which we adopted as precursors that readily converted to the crystalline films upon low-temperature in situ heating. The colloidal solutions allow for the fabrication of compact and uniform MOF films on a great deal of substrates such as fluorine-doped tin oxide, glass, SiO2, Al2O3, Si, Cu, and even flexible polycarbonate, widening their technological applications where substrates are essential. Despite the present work focuses on the fabrication of uniform cobalt-(2-methylimidazole)2 and zinc-(2-methylimidazole)2 films, our findings mark a great possibility in producing other high-quality MOF thin films on a large scale.
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