| 1. |
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| 2. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
- Blitzblau, Hannah G., et al.
(författare)
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Production of 10-methyl branched fatty acids in yeast
- 2021
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Ingår i: Biotechnology for Biofuels. - : Springer Science and Business Media LLC. - 1754-6834 .- 1754-6834. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: Despite the environmental value of biobased lubricants, they account for less than 2% of global lubricant use due to poor thermo-oxidative stability arising from the presence of unsaturated double bonds. Methyl branched fatty acids (BFAs), particularly those with branching near the acyl-chain mid-point, are a high-performance alternative to existing vegetable oils because of their low melting temperature and full saturation. Results: We cloned and characterized two pathways to produce 10-methyl BFAs isolated from actinomycetes and γ-proteobacteria. In the two-step bfa pathway of actinomycetes, BfaB methylates Δ9 unsaturated fatty acids to form 10-methylene BFAs, and subsequently, BfaA reduces the double bond to produce a fully saturated 10-methyl branched fatty acid. A BfaA-B fusion enzyme increased the conversion efficiency of 10-methyl BFAs. The ten-methyl palmitate production (tmp) pathway of γ-proteobacteria produces a 10-methylene intermediate, but the TmpA putative reductase was not active in E. coli or yeast. Comparison of BfaB and TmpB activities revealed a range of substrate specificities from C14-C20 fatty acids unsaturated at the Δ9, Δ10 or Δ11 position. We demonstrated efficient production of 10-methylene and 10-methyl BFAs in S. cerevisiae by secretion of free fatty acids and in Y. lipolytica as triacylglycerides, which accumulated to levels more than 35% of total cellular fatty acids. Conclusions: We report here the characterization of a set of enzymes that can produce position-specific methylene and methyl branched fatty acids. Yeast expression of bfa enzymes can provide a platform for the large-scale production of branched fatty acids suitable for industrial and consumer applications.
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| 4. |
- Gao, Yan, et al.
(författare)
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Perovskite Hydroxide CoSn(OH)(6) Nanocubes for Efficient Photoreduction of CO2 to CO
- 2018
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Ingår i: ACS Sustainable Chemistry and Engineering. - : AMER CHEMICAL SOC. - 2168-0485. ; 6:1, s. 781-786
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Tidskriftsartikel (refereegranskat)abstract
- Perovskite hydroxide CoSn(OH)(6) nanoparticles were synthesized and used for the first time in the photocatalytic reduction of CO2 to CO. Under mild reaction conditions and using [Ru(bpy)(3)](PF6)(2) as the photosensitizer, a high photocatalytic efficiency of 19.3 mu mol for CO evolution with a high selectivity of 86.46% was obtained. The photocatalytic TEOA activity and CO selectivity were further improved by adding weak Bronsted acids, as proton sources, to the system.
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| 5. |
- Hou, Jungang, et al.
(författare)
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Graphene Dots Embedded Phosphide Nanosheet-Assembled Tubular Arrays for Efficient and Stable Overall Water Splitting
- 2017
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Ingår i: ACS Applied Materials and Interfaces. - : AMER CHEMICAL SOC. - 1944-8244 .- 1944-8252. ; 9:29, s. 24600-24607
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Tidskriftsartikel (refereegranskat)abstract
- Bifunctional electrocatalysts are highly desired for overall water splitting. Herein, the design and fabrication of three-dimensional (3D) hierarchical earth-abundant transition bimetallic phosphide arrays constructed by one-dimensional tubular array that was derived from assembling two-dimensional nanosheet framework has been reported by tailoring the Co/Ni ratio and tunable morphologies, and zero-dimensional (0D) graphene dots were embedded on Co-Ni phosphide matrix to construct 0D/2D tubular array as a highly efficient electrode in the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER). On the basis of advanced merits, such as the high surface-active sites, well-dispersed graphene dots, and enhanced electron transfer capacity as well as the confinement effect of the graphene dots on the nanosheets, the integrated GDs/Co0.8Ni0.2P tubular arrays as anode and cathode exhibit excellent OER and HER performance. By use of GDs/Co0.8Ni0.2 arrays in the two-electrode setup of the device, a remarkable electrocatalytic performance for full water splitting has been achieved with a high current density of 10 mA cm-2 at 1.54 V and outstanding long-term operation stability in an alkaline environment, indicating a promising system based on nonprecious-metal electrocatalysts toward potential practical devices of overall water splitting.
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| 6. |
- Huang, Letian, et al.
(författare)
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A Lifetime-aware Mapping Algorithm to Extend MTTF of Networks-on-Chip
- 2018
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Ingår i: 2018 23rd Asia and South Pacific Design Automation Conference Proceedings (ASP-DAC). - : Institute of Electrical and Electronics Engineers (IEEE). - 9781509006021 ; , s. 147-152
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Konferensbidrag (refereegranskat)abstract
- Fast aging of components has become one of the major concerns in Systems-on-Chip with further scaling of the submicron technology. This problem accelerates when combined with improper working conditions such as unbalanced components' utilization. Considering the mapping algorithms in the Networks-on-Chip domain, some routers/links might be frequently selected for mapping while others are underutilized. Consequently, the highly utilized components may age faster than others which results in disconnecting the related cores from the network. To address this issue, we propose a mapping algorithm, called lifetime-aware neighborhood allocation (LaNA), that takes the aging of components into account when mapping applications. The proposed method is able to balance the wear-out of NoC components, and thus extending the service time of NoC. We model the lifetime as a resource consumed over time and accordingly define the lifetime budget metric. LaNA selects a suitable node for mapping which has the maximum lifetime budget. Experimental results show that the lifetime-aware mapping algorithm could improve the minimal MTTF of NoC around 72.2%, 58.3%, 46.6% and 48.2% as compared to NN, CoNA, WeNA and CASqA, respectively.
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| 7. |
- Jiang, Shuyan, et al.
(författare)
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Optimizing Dynamic Mapping Techniques for On-Line NoC Test
- 2018
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Ingår i: 2018 23RD ASIA AND SOUTH PACIFIC DESIGN AUTOMATION CONFERENCE (ASP-DAC). - : IEEE. - 9781509006021 ; , s. 227-232
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Konferensbidrag (refereegranskat)abstract
- With the aggressive scaling of submicron technology, intermittent faults are becoming one of the limiting factors in achieving a high reliability in Network-on-Chip (NoC). Increasing test frequency is necessary to detect intermittent faults, which in turn interrupts the execution of applications. On the other hand, the main goal of traditional mapping algorithms is to allocate applications to the NoC platform, ignoring about the test requirement. In this paper, we propose a novel testing-aware mapping algorithm (TAMA) for NoC, targeting intermittent faults on the paths between crossbars. In this approach, the idle links are identified and the components between two crossbars are tested when the application is mapped to the platform. The components can be tested if there is enough time from when the application leaves the platform and a new application enters it. The mapping algorithm is tuned to give a higher priority to the tested paths in the next application mapping. This leaves enough time to test the links and the belonging components that have not been tested in the expected time. Experiment results show that the proposed testing-aware mapping algorithm leads to a significant improvement over FF, NN, CoNA, and WeNA.
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| 8. |
- Jiang, Shuyan, et al.
(författare)
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Testing aware dynamic mapping for path-centric network-on-chip test
- 2019
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Ingår i: Integration. - : Elsevier. - 0167-9260 .- 1872-7522. ; 67, s. 134-143
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Tidskriftsartikel (refereegranskat)abstract
- With the aggressive scaling of submicron technology, intermittent faults are becoming one of the limiting factors in achieving high reliability in Network-on-Chip (NoC). Increasing test frequency is necessary to detect intermittent faults, which in turn interrupts the execution of applications. On the other hand, the primary goal of traditional mapping algorithms is to allocate applications to the NoC platform, ignoring the test requirement. In this paper, we propose a novel testing-aware mapping algorithm (TAMA) for NoC, targeting intermittent faults on the paths between crossbars. In this approach, the idle paths are identified, and the components between two crossbars are tested when the application is mapped to the platform. The components can be tested if there is enough time from the time when the application leaves the platform to the time when a new application enters it. The mapping algorithm is tuned to give a higher priority to the tested paths in the next application mapping, which leaves enough time to test the links and the belonging components that have not been tested in the expected time. Experiment results show that the proposed testing-aware mapping algorithm leads to a significant improvement over FF(Fiexitrst Free), NN(Nearest Neighbor), CoNA(Contiguous Neighborhood Allocation), and WeNA(Weighted-based Neighborhood Allocation).
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| 9. |
- Wu, Lele, et al.
(författare)
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Organic matter composition and stability in estuarine wetlands depending on soil salinity
- 2024
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Ingår i: Science of the Total Environment. - 0048-9697 .- 1879-1026. ; 945
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Tidskriftsartikel (refereegranskat)abstract
- Coastal wetlands are key players in mitigating global climate change by sequestering soil organic matter. Soil organic matter consists of less stable particulate organic matter (POM) and more stable mineral-associated organic matter (MAOM). The distribution and drivers of MAOM and POM in coastal wetlands have received little attention, despite the processes and mechanisms differ from that in the upland soils. We explored the distribution of POM and MAOM, their contributions to SOM, and the controlling factors along a salinity gradient in an estuarine wetland. In the estuarine wetland, POM C and N were influenced by soil depth and vegetation type, whereas MAOM C and N were influenced only by vegetation type. In the estuarine wetland, SOM was predominantly in the form of MAOM (> 70 %) and increased with salinity (70 %–76 %), leading to long-term C sequestration. Both POM and MAOM increased with SOM, and the increase rate of POM was higher than that of MAOM. Aboveground plant biomass decreased with increasing salinity, resulted in a decrease in POM C (46 %–81 %) and N (52 %–82 %) pools. As the mineral amount and activity, and microbial biomass decreased, the MAOM C (2.5 %–64 %) and N pool (8.6 %–59 %) decreased with salinity. When evaluating POM, the most influential factors were microbial biomass carbon (MBC) and dissolved organic carbon (DOC). Key parameters, including MBC, DOC, soil salinity, soil water content, aboveground plant biomass, mineral content and activity, and bulk density, were identified as influencing factors for both MAOM abundance. Soil water content not only directly controlled MAOM, but together with salinity also indirectly regulated POM and MAOM by controlling microbial biomass and aboveground plant biomass. Our findings have important implications for improving the accumulation and increased stability of soil organic matter in coastal wetlands, considering the global sea level rise and increased frequency of inundation.
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| 10. |
- Xu, Yalong, et al.
(författare)
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Simultaneously Improved Efficiency and Stability in All-Polymer Solar Cells by a P-i-N Architecture
- 2019
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Ingår i: ACS Energy Letters. - : AMER CHEMICAL SOC. - 2380-8195. ; 4:9, s. 2277-2286
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Tidskriftsartikel (refereegranskat)abstract
- All-polymer organic solar cells offer exceptional stability. Unfortunately, the use of bulk heterojunction (BHJ) structure has the intrinsic challenge to control the side-chain entanglement and backbone orientation to achieve sophisticated phase separation in all-polymer blends. Here, we revealed that the P-i-N structure can outperform the BHJ ones with a nearly 50% efficiency improvement, reaching a power conversion efficiency approaching 10%. This P-i-N structure can also provide an enhanced internal electric field and remarkably stable morphology Sequential deposition under harsh thermal stress. We have further demonstrated generality of the P-i-N structure in several other all-polymer systems. Considering the adjustable polymer molecular weight and solubility, the P-i-N device structure can be more beneficial for all-polymer systems. With the design of more crystalline polymers, the antiquated P-i-N structure can further show its strength in all-polymer systems by simplified morphology control and improved carrier extraction, becoming a more favorite device structure than the dominant BHJ structure.
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