| 1. |
- Chawade, Aakash, 1980, et al.
(författare)
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Development and characterization of an oat TILLING-population and identification of mutations in lignin and beta-glucan biosynthesis genes
- 2010
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Ingår i: BMC Plant Biology. - : Springer Science and Business Media LLC. - 1471-2229. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Background: Oat, Avena sativa is the sixth most important cereal in the world. Presently oat is mostly used as feed for animals. However, oat also has special properties that make it beneficial for human consumption and has seen a growing importance as a food crop in recent decades. Increased demand for novel oat products has also put pressure on oat breeders to produce new oat varieties with specific properties such as increased or improved beta-glucan-, antioxidant-and omega-3 fatty acid levels, as well as modified starch and protein content. To facilitate this development we have produced a TILLING (Targeting Induced Local Lesions IN Genomes) population of the spring oat cultivar SW Belinda. Results: Here a population of 2600 mutagenised M2 lines, producing 2550 M3 seed lots were obtained. The M2 population was initially evaluated by visual inspection and a number of different phenotypes were seen ranging from dwarfs to giants, early flowering to late flowering, leaf morphology and chlorosis. Phloroglucinol/HCl staining of M3 seeds, obtained from 1824 different M2 lines, revealed a number of potential lignin mutants. These were later confirmed by quantitative analysis. Genomic DNA was prepared from the M2 population and the mutation frequency was determined. The estimated mutation frequency was one mutation per 20 kb by RAPD-PCR fingerprinting, one mutation per 38 kb by MALDI-TOF analysis and one mutation per 22.4 kb by DNA sequencing. Thus, the overall mutation frequency in the population is estimated to be one mutation per 20-40 kb, depending on if the method used addressed the whole genome or specific genes. During the investigation, 6 different mutations in the phenylalanine ammonia-lyase (AsPAL1) gene and 10 different mutations in the cellulose synthase-like (AsCslF6) beta-glucan biosynthesis gene were identified. Conclusion: The oat TILLING population produced in this work carries, on average, hundreds of mutations in every individual gene in the genome. It will therefore be an important resource in the development of oat with specific characters. The population (M5) will be available for academic research via Nordgen http://www.nordgen.org as soon as enough seeds are obtained.
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| 2. |
- Chen, Tingsu, et al.
(författare)
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A 2 GHz - 8.7 GHz Wideband Balun-LNA with Noise Cancellation and Gain Boosting
- 2012
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Ingår i: PRIME 2012: Proceedings of the 8th Coference on Ph.D. Research in Microelectronics and Electronics, 2012. - : IEEE conference proceedings. - 9783800734429 ; , s. 59-62
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Konferensbidrag (refereegranskat)abstract
- A wideband Balun-LNA covering the operation frequency range of magnetic tunnel junction Spin Torque Oscillator is presented. The LNA is a combination of common-source and cross-coupled common-gate stages, which provides wideband matching and noise cancellation, as well as gain boosting. The internal feedback introduced by the cross-coupling allows an additional degree of freedom to select transistor sizes and bias by decoupling the impedance matching, noise, and gain imbalance trade-offs which are present in similar topologies. Two LNAs using the proposed technique are designed in 65nm CMOS. The LNAs have a simulated bandwidth of 2 GHz - 8.7 GHz, gain of 16 dB, IIP3 of -3.5 dBm, and NF < 3.8 dB while consuming 3.72 mW from a 1.2 V power supply.
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| 3. |
- Chen, Tingsu, et al.
(författare)
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A highly tunable microwave oscillator based on MTJ STO technology
- 2014
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Ingår i: Microwave and optical technology letters (Print). - : Wiley. - 0895-2477 .- 1098-2760. ; 56:9, s. 2092-2095
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Tidskriftsartikel (refereegranskat)abstract
- This article presents a fully ESD-protected, highly tunable microwave oscillator based on magnetic tunnel junction (MTJ) spin torque oscillator (STO) technology. The oscillator consists of a compact MTJ STO and a 65 nm CMOS wideband amplifier, which amplifies the RF signal of the MTJ STO to a level that can be used to drive a PLL. The (MTJ STO+amplifier IC) pair shows a measured quality factor (Q) of 170 and a wide tunability range from 3 to 7 GHz, which demonstrate its potential to be used as a microwave oscillator in multiband, multistandard radios.
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| 4. |
- Chen, Tingsu, 1987-, et al.
(författare)
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An Inductorless Wideband Balun-LNA for Spin Torque Oscillator-based Field Sensing
- 2014
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Ingår i: Electronics, Circuits and Systems (ICECS), 2014 21st IEEE International Conference on. - : IEEE conference proceedings. - 9781479942435 ; , s. 36-39
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Konferensbidrag (refereegranskat)abstract
- This paper presents a wideband inductorless Balun-LNA targeting spin torque oscillator-based magnetic field sensing applications. The LNA consistsof a CS stage combined with a cross-coupled CG stage, which offers wideband matching, noise/distortion cancellation and gain boosting, simultaneously. The Balun-LNA is implemented in a 65 nm CMOS technology, and it is fully ESD-protected and packaged. Measurement results show a bandwidth of 2 GHz - 7 GHz, a voltage gain of 20 dB, an IIP3 of +2 dBm, and a maximum NF of 5 dB. The LNA consumes 3.84 mW from a 1.2 V power supply and occupies a total silicon area of 0.0044 mm2. The measurement results demonstrate that the proposed Balun-LNA is highly suitable for the STO-based field sensing applications.
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| 5. |
- Chen, Tingsu
(författare)
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CMOS High Frequency Circuits for Spin Torque Oscillator Technology
- 2014
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Spin torque oscillator (STO) technology has a unique blend of features, including but not limited to octave tunability, GHz operating frequency, and nanoscaled size, which makes it highly suitable for microwave and radar applications. This thesis studies the fundamentals of STOs, utilizes the state-of-art STO's advantages, and proposes two STO-based microwave systems targeting its microwave applications and measurement setup, respectively.First, based on an investigation of possible STO applications, the magnetic tunnel junction (MTJ) STO shows a great suitability for microwave oscillator in multi-standard multi-band radios. Yet, it also imposes a large challenge due to its low output power, which limits it from being used as a microwave oscillator. In this regard, different power enhancement approaches are investigated to achieve an MTJ STO-based microwave oscillator. The only possible approach is to use a dedicated CMOS wideband amplifier to boost the output power of the MTJ STO. The dedicated wideband amplifier, containing a novel Balun-LNA, an amplification stage and an output buffer, is proposed, analyzed, implemented, measured and used to achieve the MTJ STO-based microwave oscillator. The proposed amplifier core consumes 25.44 mW from a 1.2 V power supply and occupies an area of 0.16 mm2 in a 65 nm CMOS process. The measurement results show a S21 of 35 dB, maximum NF of 5 dB, bandwidth of 2 GHz - 7 GHz. This performance, as well as the measurement results of the proposed MTJ STO-based microwave oscillator, show that this microwave oscillator has a highly-tunable range and is able to drive a PLL.The second aspect of this thesis, firstly identifies the major difficulties in measuring the giant magnetoresistance (GMR) STO, and hence studying its dynamic properties. Thereafter, the system architecture of a reliable GMR STO measurement setup, which integrates the GMR STO with a dedicated CMOS high frequency IC to overcome these difficulties in precise characterization of GMR STOs, is proposed. An analysis of integration methods is given and the integration method based on wire bonding is evaluated and employed, as a first integration attempt of STO and CMOS technologies. Moreover, a dedicated high frequency CMOS IC, which is composed of a dedicated on-chip bias-tee, ESD diodes, input and output networks, and an amplification stage for amplifying the weak signal generated by the GMR STO, is proposed, analyzed, developed, implemented and measured. The proposed dedicated high frequency circuits for GMR STO consumes 14.3 mW from a 1.2 V power supply and takes a total area of 0.329 mm2 in a 65 nm CMOS process. The proposed on-chip bias-tee presents a maximum measured S12 of -20 dB and a current handling of about 25 mA. Additionally, the proposed dedicated IC gives a measured gain of 13 dB with a bandwidth of 12.5 GHz - 14.5 GHz. The first attempt to measure the (GMR STO+IC) pair presents no RF signal at the output. The possible cause and other identified issues are given.
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| 6. |
- Chen, Tingsu, et al.
(författare)
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Comprehensive and Macrospin-Based Magnetic Tunnel Junction Spin Torque Oscillator Model-Part I : Analytical Model of the MTJ STO
- 2015
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Ingår i: IEEE Transactions on Electron Devices. - 0018-9383 .- 1557-9646. ; 62:3, s. 1037-1044
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Tidskriftsartikel (refereegranskat)abstract
- Magnetic tunnel junction (MTJ) spin torque oscillators (STOs) have shown the potential to be used in a wide range of microwave and sensing applications. To evaluate the potential uses of MTJ STO technology in various applications, an analytical model that can capture MTJ STO's characteristics, while enabling system-and circuit-level designs, is of great importance. An analytical model based on macrospin approximation is necessary for these designs since it allows implementation in hardware description languages. This paper presents a new macrospin-based, comprehensive, and compact MTJ STO model, which can be used for various MTJ STOs to estimate the performance of MTJ STOs together with their application-specific integrated circuits. To adequately present the complete model, this paper is divided into two parts. In Part I, the analytical model is introduced and verified by comparing it against measured data of three different MTJ STOs, varying the angle and magnitude of the magnetic field, as well as the DC biasing current. The proposed analytical model is suitable for being implemented in Verilog-A and used for efficient simulations at device, circuit, and system levels. In Part II, the full Verilog-A implementation of the analytical model with accurate phase noise generation is presented and verified by simulations.
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| 7. |
- Chen, Tingsu, et al.
(författare)
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Comprehensive and Macrospin-Based Magnetic Tunnel Junction Spin Torque Oscillator Model-Part II : Verilog-A Model Implementation
- 2015
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Ingår i: IEEE Transactions on Electron Devices. - 0018-9383 .- 1557-9646. ; 62:3, s. 1045-1051
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Tidskriftsartikel (refereegranskat)abstract
- The rapid development of the magnetic tunnel junction (MTJ) spin torque oscillator (STO) technology demands an analytical model to enable building MTJ STO-based circuits and systems so as to evaluate and utilize MTJ STOs in various applications. In Part I of this paper, an analytical model based on the macrospin approximation has been introduced and verified by comparing it with the measurements of three different MTJ STOs. In Part II, the full Verilog-A implementation of the proposed model is presented. To achieve a reliable model, an approach to reproducing the phase noise generated by the MTJ STO has been proposed and successfully employed. The implemented model yields a time domain signal, which retains the characteristics of operating frequency, linewidth, oscillation amplitude, and DC operating point, with respect to the magnetic field and applied DC current. The Verilog-A implementation is verified against the analytical model, providing equivalent device characteristics for the full range of biasing conditions. Furthermore, a system that includes an MTJ STO and CMOS RF circuits is simulated to validate the proposed model for system-and circuit-level designs. The simulation results demonstrate that the proposed model opens the possibility to explore STO technology in a wide range of applications.
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| 8. |
- Chen, Tingsu, et al.
(författare)
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Identification of gliadin-binding peptides by phage display
- 2011
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Ingår i: BMC Biotechnology. - : Springer Science and Business Media LLC. - 1472-6750. ; 11
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Coeliac disease (CD) is a common and complex disorder of the small intestine caused by intolerance to wheat gluten and related edible cereals like barley and rye. Peptides originating from incomplete gliadin digestion activate the lamina propria infiltrating T cells to release proinflammatory cytokines, which in turn cause profound tissue remodelling of the small intestinal wall. There is no cure for CD except refraining from consuming gluten-containing products. RESULTS: Phage from a random oligomer display library were enriched by repeated pannings against immobilised gliadin proteins. Phage from the final panning round were plated, individual plaques picked, incubated with host bacteria, amplified to a population size of 1011 to 1012 and purified. DNA was isolated from 1000 purified phage populations and the region covering the 36 bp oligonucleotide insert from which the displayed peptides were translated, was sequenced. Altogether more than 150 different peptide-encoding sequences were identified, many of which were repeatedly isolated under various experimental conditions. Amplified phage populations, each expressing a single peptide, were tested first in pools and then one by one for their ability to inhibit binding of human anti-gliadin antibodies in ELISA assays. These experiments showed that several of the different peptide-expressing phage tested inhibited the interaction between gliadin and anti-gliadin antibodies. Finally, four different peptide-encoding sequences were selected for further analysis, and the corresponding 12-mer peptides were synthesised in vitro. By ELISA assays it was demonstrated that several of the peptides inhibited the interaction between gliadin molecules and serum anti-gliadin antibodies. Moreover, ELISA competition experiments as well as dot-blot and western blot revealed that the different peptides interacted with different molecular sites of gliadin. CONCLUSIONS: We believe that several of the isolated and characterised gliadin-binding peptides described here could provide valuable tools for researchers in the field of CD by facilitating studies on localisation and uptake of various gliadin peptides in the small intestine. In future work, the potential of these peptides to detoxify gluten will be investigated.
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| 9. |
- Chen, Tingsu, et al.
(författare)
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Integration of GMR-based spin torque oscillators and CMOS circuitry
- 2015
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Ingår i: Solid-State Electronics. - : Elsevier BV. - 0038-1101 .- 1879-2405. ; 111, s. 91-99
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Tidskriftsartikel (refereegranskat)abstract
- This paper demonstrates the integration of giant magnetoresistance (GMR) spin torque oscillators (STO) with dedicated high frequency CMOS circuits. The wire-bonding-based integration approach is employed in this work, since it allows easy implementation, measurement and replacement. A GMR STO is wire-bonded to the dedicated CMOS integrated circuit (IC) mounted on a PCB, forming a (GMR STO + CMOS IC) pair. The GMR STO has a lateral size of 70 nm and more than an octave of tunability in the microwave frequency range. The proposed CMOS IC provides the necessary bias-tee for the GMR STO, as well as electrostatic discharge (ESD) protection and wideband amplification targeting high frequency GMR STO-based applications. It is implemented in a 65 nm CMOS process, offers a measured gain of 12 dB, while consuming only 14.3 mW and taking a total silicon area of 0.329 mm(2). The measurement results show that the (GMR STO + CMOS IC) pair has a wide tunability range from 8 GHz to 16.5 GHz and improves the output power of the GMR STO by about 10 dB. This GMR STO-CMOS integration eliminates wave reflections during the signal transmission and therefore exhibits good potential for developing high frequency GMR STO-based applications, which combine the features of CMOS and STO technologies. (C) 2015 Elsevier Ltd. All rights reserved.
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| 10. |
- Chen, Tingsu, et al.
(författare)
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Spin-Torque and Spin-Hall Nano-Oscillators
- 2016
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Ingår i: Proceedings of the IEEE. - : Institute of Electrical and Electronics Engineers (IEEE). - 0018-9219 .- 1558-2256. ; 104:10, s. 1919-1945
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Tidskriftsartikel (refereegranskat)abstract
- This paper reviews the state of the art in spin-torque and spin-Hall-effect-driven nano-oscillators. After a brief introduction to the underlying physics, the authors discuss different implementations of these oscillators, their functional properties in terms of frequency range, output power, phase noise, and modulation rates, and their inherent propensity for mutual synchronization. Finally, the potential for these oscillators in a wide range of applications, from microwave signal sources and detectors to neuromorphic computation elements, is discussed together with the specific electronic circuitry that has so far been designed to harness this potential.
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