| 1. |
- Xu, An, et al.
(författare)
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Rewired m6A epitranscriptomic networks link mutant p53 to neoplastic transformation
- 2023
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Ingår i: Nature Communications. - : Springer Nature. - 2041-1723. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- N6-methyladenosine (m6A), one of the most prevalent mRNA modifications in eukaryotes, plays a critical role in modulating both biological and pathological processes. However, it is unknown whether mutant p53 neomorphic oncogenic functions exploit dysregulation of m6A epitranscriptomic networks. Here, we investigate Li-Fraumeni syndrome (LFS)-associated neoplastic transformation driven by mutant p53 in iPSC-derived astrocytes, the cell-of-origin of gliomas. We find that mutant p53 but not wild-type (WT) p53 physically interacts with SVIL to recruit the H3K4me3 methyltransferase MLL1 to activate the expression of m6A reader YTHDF2, culminating in an oncogenic phenotype. Aberrant YTHDF2 upregulation markedly hampers expression of multiple m6A-marked tumor-suppressing transcripts, including CDKN2B and SPOCK2, and induces oncogenic reprogramming. Mutant p53 neoplastic behaviors are significantly impaired by genetic depletion of YTHDF2 or by pharmacological inhibition using MLL1 complex inhibitors. Our study reveals how mutant p53 hijacks epigenetic and epitranscriptomic machinery to initiate gliomagenesis and suggests potential treatment strategies for LFS gliomas.
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| 2. |
- Kristan, Matej, et al.
(författare)
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The Visual Object Tracking VOT2017 challenge results
- 2017
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Ingår i: 2017 IEEE INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW 2017). - : IEEE. - 9781538610343 ; , s. 1949-1972
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2017 is the fifth annual tracker benchmarking activity organized by the VOT initiative. Results of 51 trackers are presented; many are state-of-the-art published at major computer vision conferences or journals in recent years. The evaluation included the standard VOT and other popular methodologies and a new "real-time" experiment simulating a situation where a tracker processes images as if provided by a continuously running sensor. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The VOT2017 goes beyond its predecessors by (i) improving the VOT public dataset and introducing a separate VOT2017 sequestered dataset, (ii) introducing a realtime tracking experiment and (iii) releasing a redesigned toolkit that supports complex experiments. The dataset, the evaluation kit and the results are publicly available at the challenge website(1).
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| 3. |
- Wang, Shangshang, et al.
(författare)
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Cu2+-Selectivity gated photochromism in Schiff-modified diarylethenes with a star-shaped structure
- 2017
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Ingår i: Journal of Materials Chemistry C. - : Royal Society of Chemistry (RSC). - 2050-7526 .- 2050-7534. ; 5:2, s. 282-289
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Tidskriftsartikel (refereegranskat)abstract
- A great deal of effort has been devoted to developing gated photochromic systems due to their advantages in the smart materials and opto-electronic fields, whereas the gating function through certain ions has rarely been addressed. Since the photochromic materials gated by ions can be readily further processed into a multi-functional molecular switch and probe, we herein designed and conveniently synthesized a star-shaped Schiff-based diarylethene derivative showing typical photochromic properties in solution. This compound possesses two response channels (colorimetric and fluorogenic) to Cu2+ ions with photoswitching characteristics, making it a viable photochromic probe. It is noteworthy that its photochromic reactivity can be locked when Cu2+ ions are introduced into the solution. Moreover, the photoinactive and photoactive states can be interchanged reversibly by binding Cu2+ ions and unbinding Cu2+ ions using EDTA, which shows promise for application in multi-controlled molecular switches and smart materials. The mechanism of the photochromic properties locked by Cu2+ ions is reasonably proposed by theoretical simulations. These results could be valuable for the further development of molecular switching systems with multiple stimuli responses.
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| 4. |
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| 5. |
- Chen, Ming, et al.
(författare)
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Failure mechanism of rock mass in bench blasting based on structural dynamics
- 2021
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Ingår i: Bulletin of Engineering Geology and the Environment. - : Springer. - 1435-9529 .- 1435-9537. ; 80:9, s. 6841-6861
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Tidskriftsartikel (refereegranskat)abstract
- This study establishes a multiple degrees-of-freedom structural dynamics analytical model to analyse the influence mechanism of different factors on blasting tight bottom and shape of muckpile. The structural displacement response and distribution of internal forces in the bench rock mass are analysed based on several factors including blasting parameters, explosion load, initiation condition, and geological condition. In addition, the structural failure characteristics of the bench rock mass are studied based on a rock strength criterion. The results indicate that the explosive load strength determines the internal forces of the bench rock mass. The use of blasting parameters with large borehole spacing and small row spacing can increase the internal force and deformation of the bench rock and enhance the effect of the breaking and throwing of rock mass. In addition, the strengthening of the lithology of the bottom rocks or weakening of the lithology of the middle rocks can make destroying the bottom rock mass more difficult and increase the probability of blasting tight bottom formation. Adjusting the initiation point to below the weak-lithology segment of the bench can enhance the internal force and displacement of the bottom rock mass, to improve the blasting effect and avoid blasting tight bottom formation. Combined with the bench blasting field test of the Changjiu limestone mine, it verifies the results of the theoretical analysis of the bench blasting rock mass destruction based on structural dynamics. The results can be used as the theoretical basis and technical support for improving the bench blasting effect.
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| 6. |
- Chen, Wenbo, et al.
(författare)
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The draft genome of whitefly Bemisia tabaci MEAM1, a global crop pest, provides novel insights into virus transmission, host adaptation, and insecticide resistance
- 2016
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Ingår i: BMC Biology. - : Springer Science and Business Media LLC. - 1741-7007. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: The whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is among the 100 worst invasive species in the world. As one of the most important crop pests and virus vectors, B. tabaci causes substantial crop losses and poses a serious threat to global food security. Results: We report the 615-Mb high-quality genome sequence of B. tabaci Middle East-Asia Minor 1 (MEAM1), the first genome sequence in the Aleyrodidae family, which contains 15,664 protein-coding genes. The B. tabaci genome is highly divergent from other sequenced hemipteran genomes, sharing no detectable synteny. A number of known detoxification gene families, including cytochrome P450s and UDP-glucuronosyltransferases, are significantly expanded in B. tabaci. Other expanded gene families, including cathepsins, large clusters of tandemly duplicated B. tabaci-specific genes, and phosphatidylethanolamine-binding proteins (PEBPs), were found to be associated with virus acquisition and transmission and/or insecticide resistance, likely contributing to the global invasiveness and efficient virus transmission capacity of B. tabaci. The presence of 142 horizontally transferred genes from bacteria or fungi in the B. tabaci genome, including genes encoding hopanoid/sterol synthesis and xenobiotic detoxification enzymes that are not present in other insects, offers novel insights into the unique biological adaptations of this insect such as polyphagy and insecticide resistance. Interestingly, two adjacent bacterial pantothenate biosynthesis genes, panB and panC, have been co-transferred into B. tabaci and fused into a single gene that has acquired introns during its evolution. Conclusions: The B. tabaci genome contains numerous genetic novelties, including expansions in gene families associated with insecticide resistance, detoxification and virus transmission, as well as numerous horizontally transferred genes from bacteria and fungi. We believe these novelties likely have shaped B. tabaci as a highly invasive polyphagous crop pest and efficient vector of plant viruses. The genome serves as a reference for resolving the B. tabaci cryptic species complex, understanding fundamental biological novelties, and providing valuable genetic information to assist the development of novel strategies for controlling whiteflies and the viruses they transmit.
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| 7. |
- Hancock, Dana B, et al.
(författare)
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Genome-Wide Joint Meta-Analysis of SNP and SNP-by-Smoking Interaction Identifies Novel Loci for Pulmonary Function
- 2012
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Ingår i: PLoS genetics. - : Public Library of Science (PLoS). - 1553-7404. ; 8:12, s. e1003098-
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Tidskriftsartikel (refereegranskat)abstract
- Genome-wide association studies have identified numerous genetic loci for spirometic measures of pulmonary function, forced expiratory volume in one second (FEV1), and its ratio to forced vital capacity (FEV1/FVC). Given that cigarette smoking adversely affects pulmonary function, we conducted genome-wide joint meta-analyses (JMA) of single nucleotide polymorphism (SNP) and SNP-by-smoking (ever-smoking or pack-years) associations on FEV1 and FEV1/FVC across 19 studies (total N = 50,047). We identified three novel loci not previously associated with pulmonary function. SNPs in or near DNER (smallest PJMA = 5.00×10−11), HLA-DQB1 and HLA-DQA2 (smallest PJMA = 4.35×10−9), and KCNJ2 and SOX9 (smallest PJMA = 1.28×10−8) were associated with FEV1/FVC or FEV1 in meta-analysis models including SNP main effects, smoking main effects, and SNP-by-smoking (ever-smoking or pack-years) interaction. The HLA region has been widely implicated for autoimmune and lung phenotypes, unlike the other novel loci, which have not been widely implicated. We evaluated DNER, KCNJ2, and SOX9 and found them to be expressed in human lung tissue. DNER and SOX9 further showed evidence of differential expression in human airway epithelium in smokers compared to non-smokers. Our findings demonstrated that joint testing of SNP and SNP-by-environment interaction identified novel loci associated with complex traits that are missed when considering only the genetic main effects.
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| 8. |
- Kristan, Matej, et al.
(författare)
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The Visual Object Tracking VOT2016 Challenge Results
- 2016
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Ingår i: COMPUTER VISION - ECCV 2016 WORKSHOPS, PT II. - Cham : SPRINGER INT PUBLISHING AG. - 9783319488813 - 9783319488806 ; , s. 777-823
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2016 aims at comparing short-term single-object visual trackers that do not apply pre-learned models of object appearance. Results of 70 trackers are presented, with a large number of trackers being published at major computer vision conferences and journals in the recent years. The number of tested state-of-the-art trackers makes the VOT 2016 the largest and most challenging benchmark on short-term tracking to date. For each participating tracker, a short description is provided in the Appendix. The VOT2016 goes beyond its predecessors by (i) introducing a new semi-automatic ground truth bounding box annotation methodology and (ii) extending the evaluation system with the no-reset experiment.
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| 9. |
- Wu, Hongwei, et al.
(författare)
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Tuning for Visible Fluorescence and Near-Infrared Phosphorescence on a Unimolecular Mechanically Sensitive Platform via Adjustable CH-pi Interaction
- 2017
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Ingår i: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 9:4, s. 3865-3872
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Tidskriftsartikel (refereegranskat)abstract
- CH-pi interaction-assisted alignment of organic conjugated systems has played an important role to regulate molecular electronic and photophysical properties, whereas harnessing such a smart noncovalent interaction into the tuning of unimolecular complex emissive bands covering a wide spectral region remains a challenging research topic. Since the tuning for visible and near-infrared emissive properties in a single pi-functional platform relates to its multicolor luminescent behaviors and potential superior application in analysis, bioimaging, and sensing, herein, we report a proportional control of the singlet and triplet emissions that cover visible and near-infrared spectral regions, respectively, can be straightforwardly achieved by CH-pi interaction-assisted self-assembly at the unimolecular level. Employing an octathionaphthalene-based single luminophore as a prototype, we find that a strength-adjustable CH-pi interaction-assisted self-assembly can be established in mixed DMF/H2O and in the film state. The hybridization of planar local excited and intramolecular charge transfer transitions occurs on the basis, allowing a competitive inhibition to the intersystem crossing process to generate a complex emission composed of visible fluorescence and near-infrared phosphorescence. Furthermore, reversible mechanochromic and mechanoluminescent conversions of the corresponding solid sample can both be observed to rely on a corresponding self-assembly alternation. These results can probably provide new visions for the development of future intelligent and multifunctional luminescent materials.
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| 10. |
- Ye, Zhiwei, et al.
(författare)
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Quantitative Study of the Action on Rock Mass Failure under the Shock Wave and Gas Pressure in Bench Blasting
- 2023
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Ingår i: International Journal of Geomechanics. - : American Society of Civil Engineers (ASCE). - 1532-3641 .- 1943-5622. ; 23:9
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Tidskriftsartikel (refereegranskat)abstract
- The expansion and shock wave coexisting failure theory has been widely recognized. However, it is not clear whether the main cause of rock mass blasting failure is the shock wave or gas pressure. In this paper, the contribution proportions of both loads to rock mass failure were investigated in bench blasting. First, the blasting damage in rock mass was simulated with a fluid-structure interaction (FSI) method. Then, a novel method to quantitatively distinguish between the rock-breaking effects (RBEs) of the shock wave and gas pressure was proposed that was based on the damage results. In addition, under different free surface conditions, the blasting failure volume that was caused by both loads was obtained for three typical rock masses, which included poor, middle, and good rock masses. The results showed that the range of the tensile failure zone by reflected waves was small, and the favorable effects of free surfaces on the failure induced by shock waves were limited. The free surface had a minor beneficial influence on the rock mass failure that was induced by the shock waves. In addition, it had a more favorable influence on the failure that was induced by the gas pressure. Finally, the influence of the free surface and rock mass conditions on the contribution proportions of both loads was discussed. A higher proportion of the RBEs of the shock wave was in the good mass with large wave impedance compared with the poor rock mass with small wave impedance. According to the contribution proportions under different rock masses and free surface conditions, the main cause of rock blasting failure was the gas pressure action, which was verified through the field high-speed photography data. The findings revealed the main cause of rock mass failure in bench blasting and could provide a theoretical basis when seeking effective engineering measures to give full play to the gas pressure action.
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