| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Chen, Hui, et al.
(författare)
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Hyperbolic CORDIC-Based Architecture for Computing Logarithm and Its Implementation
- 2020
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Ingår i: IEEE Transactions on Circuits and Systems - II - Express Briefs. - : Institute of Electrical and Electronics Engineers (IEEE). - 1549-7747 .- 1558-3791. ; 67:11, s. 2652-2656
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Tidskriftsartikel (refereegranskat)abstract
- We present a CORDIC (Coordinate Rotation Digital Computer)-based method to compute the logarithm function with base 2 and validate this method by software simulation and hardware implementation. Technically, we overcome the limitation of traditional hyperbolic CORDIC and transform it based on the idea of generalized hyperbolic CORDIC so that it can be used to compute $log_{2}x\;(x\;\epsilon \;[1,2))$ . The proposed method requires only simple shift-and-add operations and has a great tradeoff between precision (or speed) and area. In MATLAB, we provide different precisions corresponding to the iterations of the transformed CORDIC for user needs. Using a pipelined structure and setting the number of iterations to be 16 (the average relative error is $2.09\times 10<^>{-6}$ ), we implement an example hardware circuit. Synthesized under the SMIC 65nm CMOS technology, the circuit has an area of 24100 $\mu m<^>{2}$ and computation time of 11.1 ns, which can save 31.04x0025; area and improve 6.92x0025; computation speed averagely compared with existing methods.
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| 3. |
- Chen, Qinyu, et al.
(författare)
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An Efficient Streaming Accelerator for Low Bit-Width Convolutional Neural Networks
- 2019
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Ingår i: Electronics. - : MDPI. - 2079-9292. ; 8:4
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Tidskriftsartikel (refereegranskat)abstract
- Convolutional Neural Networks (CNNs) have been widely applied in various fields, such as image recognition, speech processing, as well as in many big-data analysis tasks. However, their large size and intensive computation hinder their deployment in hardware, especially on the embedded systems with stringent latency, power, and area requirements. To address this issue, low bit-width CNNs are proposed as a highly competitive candidate. In this paper, we propose an efficient, scalable accelerator for low bit-width CNNs based on a parallel streaming architecture. With a novel coarse grain task partitioning (CGTP) strategy, the proposed accelerator with heterogeneous computing units, supporting multi-pattern dataflows, can nearly double the throughput for various CNN models on average. Besides, a hardware-friendly algorithm is proposed to simplify the activation and quantification process, which can reduce the power dissipation and area overhead. Based on the optimized algorithm, an efficient reconfigurable three-stage activation-quantification-pooling (AQP) unit with the low power staged blocking strategy is developed, which can process activation, quantification, and max-pooling operations simultaneously. Moreover, an interleaving memory scheduling scheme is proposed to well support the streaming architecture. The accelerator is implemented with TSMC 40 nm technology with a core size of . It can achieve TOPS/W energy efficiency and area efficiency at 100.1mW, which makes it a promising design for the embedded devices.
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| 4. |
- Chen, Qinyu, et al.
(författare)
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Smilodon : An Efficient Accelerator for Low Bit-Width CNNs with Task Partitioning
- 2019
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Ingår i: 2019 IEEE INTERNATIONAL SYMPOSIUM ON CIRCUITS AND SYSTEMS (ISCAS). - : IEEE. - 9781728103976
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Konferensbidrag (refereegranskat)abstract
- Convolutional Neural Networks (CNNs) have been widely applied in various fields such as image and video recognition, recommender systems, and natural language processing. However, the massive size and intensive computation loads prevent its feasible deployment in practice, especially on the embedded systems. As a highly competitive candidate, low bit-width CNNs are proposed to enable efficient implementation. In this paper, we propose Smilodon, a scalable, efficient accelerator for low bit-width CNNs based on a parallel streaming architecture, optimized with a task partitioning strategy. We also present the 3D systolic-like computing arrays fitting for convolutional layers. Our design is implemented on Zynq XC7ZO20 FPGA, which can satisfy the needs of real-time with a frame rate of 1, 622 FPS throughput, while consuming 2.1 Watt. To the best of our knowledge, our accelerator is superior to the state-of-the-art works in the tradeoff among throughput, power efficiency, and area efficiency.
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| 5. |
- Hao, Zhengming, et al.
(författare)
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From n-alkane to polyacetylene on Cu (110): Linkage modulation in chain growth
- 2022
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Ingår i: Science in China Series B. - : SCIENCE PRESS. - 1674-7291 .- 1869-1870. ; 65:4, s. 733-739
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Tidskriftsartikel (refereegranskat)abstract
- Direct coupling or transformation of inert alkanes based on the selective C-H activation is of great importance for both chemistry and chemical engineering. Here, we report the coupling of polyenes that are transformed from n-dotriacontane (n-C32H66) through on-surface cascade dehydrogenation on Cu (110) surface, leading to the formation of polyacetylene (PA). Three distinct linkages have been resolved by scanning tunneling microscope (STM) and noncontact atomic force microscope (nc-AFM). Apart from the alpha-type linkage which is the stemless coupling of the terminal C-C double bond in trans-configuration, beta- and gamma-type linkages appear as knots or defects which are, in fact, the C-C couplings in cis-configurations. Interestingly, the "defects" can be effectively suppressed by adjusting the surface coverage, thus making it of general interest for uniform structure modulation.
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