| 1. |
- Bryan, Heather M, et al.
(författare)
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Identification of Parelaphostrongylus odocoilei (Nematoda: Protostrongylidae) First-Stage Larvae in the Feces of Gray Wolves (Canis lupus) by Molecular Methods
- 2010
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Ingår i: Journal of Wildlife Diseases. - 0090-3558 .- 1943-3700. ; 46:1, s. 297-302
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Tidskriftsartikel (refereegranskat)abstract
- First-stage nematode larvae with a dorsal-spine (E)SI.)),were, detected ill five of, 1,565 fecal samples from gray wolves (Canis lupus) collected ill British Columbia, Canada, between 2005 and 2008. Molecular techniques were used to identify the DSL because it was not possible to determine their species identity using morphologic characters. The DSL were identified as parelaphostrongylus odocoilei based oil the results of single-strand conformation polymorphism (SSCP) analyses and DNA sequencing of the ribosomal DNA First and second internal transcribed spacers. Finding DSL of P. odocoilei in the feces of gray wolves was unexpected because P. odocoilei adults are parasites of cervids and bovids. The most likely explanation for the presence of DSL in wolf feces is that they were ingested along with the viscera of recently consumed prey. This was probably black-tailed deer (Odocoileus hemionus columbianus), which are known in the sampling area to be hosts of P. odocoilei. The present study demonstrates the use of SSCP and DNA sequencing for the identification, to the species level, of,parasitic nematode larvae ill feces.
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| 2. |
- Díaz-Sánchez, Adrian Alberto, et al.
(författare)
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Molecular detection and characterization of Hepatozoon canis in stray dogs from Cuba
- 2021
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Ingår i: Parasitology international. - : Elsevier. - 1383-5769 .- 1873-0329. ; 80
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Tidskriftsartikel (refereegranskat)abstract
- Canine hepatozoonosis caused by Hepatozoon canis is a worldwide distributed tick-borne disease of domestic and wild canids that is transmitted by ingestion of Rhipicephalus sanguineus sensu lato (s.l.) ticks. The present study was aimed to determine the prevalence of Hepatozoon infections in 80 stray dogs from Havana Province in Cuba, and to confirm the species identity and phylogenetic relationships of the causative agent. Samples were screened by microscopical examination of thin blood smears for the presence of Hepatozoon spp. gamonts and by genus-specific SYBR green-based real-time PCR assay targeting the 18S rRNA gene. Direct microscopy examination revealed Hepatozoon gamonts in the peripheral blood of 8 dogs (10.0%; 95% CI: 4.80-18.0%), while 38 animals (47.5%; 95% CI: 36.8-58.4%) were PCR-positive, including all microscopically positive dogs. Hence, the agreement between the two detection methods was 'poor' (κ = 0.20). Hematological parameters did not differ significantly between PCR-positive and PCR-negative dogs (p > 0.05). The DNA sequences of the 18S rRNA gene of the Hepatozoon spp. from Cuban dogs showed a nucleotide identity >99% with those of 18S rRNA sequences of Hepatozoon canis isolates from Czech Republic, Brazil and Spain. Phylogenetic analysis revealed that obtained sequences clustered within the Hepatozoon canis clade, different from the Hepatozoon felis or Hepatozoon americanum clades. The present study represents the first molecular characterization of Hepatozoon canis in stray dogs within Cuba.
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| 3. |
- Díaz-Sánchez, Adrian A., et al.
(författare)
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Molecular detection and identification of tick-borne pathogens in Equus caballus and ticks from western Cuba : [Detección e identificación molecular de patógenos transmitidos por garrapatas en Equus caballus y garrapatas del occidente de Cuba]
- 2022
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Ingår i: Biotecnologia Aplicada. - : Elfos Scientiae. - 0864-4551 .- 1027-2852. ; 39:2, s. 2501-2505
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Tidskriftsartikel (refereegranskat)abstract
- Babesia caballi, Theileria equi and several species of rickettsias are agents of vector-borne diseases that affect equines. The objective of this study was to detect infections by B. caballi and T. equi in horses and to identify rickettsias in horses and ticks in the western region of Cuba. Two nPCR assays were developed and standardized for the detection of B. caballi and T. equi. Blood samples from horses and ticks were collected. Identification by blood smear and molecular detection and identification of B. caballi, T. equi and Rickettsia spp. were carried out. Intraerythrocytic formations compatible with B. caballi and T. equi were observed. The nPCR showed that 25 % of the samples were positive for B. caballi, 73 % for T. equi and 20 % showed coinfection. The results were confirmed with the partial sequencing of the genes bc48 (B. caballi) and ema-1 (T. equi). The sequencing of the 18S rRNA gene of T. equi demonstrated the presence of at least two genotypes of T. equi isolates in Cuba. The real time qPCR assay and sequencing revealed the presence of Rickettsia amblyommatis in A. mixtum and Rickettsia felis in D. nitens.Conclusions: These results constitute the first piece of molecular evidence of B. caballi and T. equi in horses and the first report of R. felis in D. nitens in Cuba, which broadens the knowledge about the distribution of pathogens and the spectrum of potential vectors contributing to the strengthening of management and control programs.
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| 4. |
- González, Belkis Corona, et al.
(författare)
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Detección e identificación molecular de patógenos transmitidos por garrapatas en perros de La Habana, Cuba : [Detection and molecular identification of tick-borne pathogens in Havana, Cubandogs]
- 2023
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Ingår i: Anales de la Academia de Ciencias de Cuba. - : Editorial Ciencias Medicas. - 2304-0106. ; 13:1
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Tidskriftsartikel (refereegranskat)abstract
- Introduction: Vector-borne canine diseases are a serious danger to animal and human health. The objective of the present study was to determine the prevalence of zoonotic pathogens (Anaplasma platys, Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Ehrlichia canis and Rickettsia spp. and non-zoonotic (Hepatozoon canis and Babesia spp.) in ownerless dogs from Havana, Cuba.Methods: Blood samples were collected from 100 dogs and the hematological profiles were studied and pathogens were detected by visualization of blood smears and real-time PCR. To determine the prevalence of infection by Hepatozoon canis, 80 dogs were studied by visualization of blood smears andreal time PCR. For the detection of Babesia spp., 60 dogs were studied, using hematological examination, blood smear visualization and PCR.Results: Eighty-five dogs were positive for at least one pathogen, being E. canis the most prevalent, followed by A. platys and Rickettsia felis, and 36 % showed coinfections. All samples were negative for A. phagocytophilum and B. burgdorferi s.l. These results constitute the first report of R. felis in dogs from Cuba, demonstrate the high prevalence of pathogens transmitted by ticks, with zoonotic potential, and constitute the first study where coinfections are analyzed. 38 % of the dogs were positive for H. canis by PCR and for the first-time molecular characterization of H. canis was carried out in Cuban ownerless dogs and 20 % were positive by PCR for Babesia spp., which constitutes the first molecular evidence of Babesia spp. in ownerless dogs fromCuba.Conclusions, these results are of great importance for the surveillance of vector-borne diseasesin dogs, and demonstrate the need for studies on the prevention of transmission and spread of the diseases they cause.
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