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- Carlborg, Carl Fredrik, et al.
(författare)
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Biosticker : patterned microfluidic stickers for rapid integration with microarrays
- 2011
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Ingår i: The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2011). - : Chemical and Biological Microsystems Society. - 9781618395955 ; , s. 311-313
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Konferensbidrag (refereegranskat)abstract
- We present a one-step, reversible, and biocompatible bonding method of a stiff patterned microfluidic "Biosticker", based on off-stoichiometry thiol-ene (OSTE) polymers [1], to state-of-the-art spotted microarray surfaces. The method aims at improving and simplifying the batch back-end processing of microarrays. We illustrate its ease of use in two applications: a high sensitivity flow-through protein assay; and a DNA-hybridization test. Read-out was performed in a standard highvolume array scanner, and showed excellent spot homogeneity and intensity. The Biosticker is aimed to be a plug-in for existing microarray platforms to enable faster protein assays and DNA hybridizations through mass transport optimization.
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