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| 2. |
- Aspholm, Marina, et al.
(författare)
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Helicobacter pylori adhesion to carbohydrates
- 2006
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Ingår i: Methods in Enzymology. - 0076-6879 .- 1557-7988. ; 417, s. 293-339
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Tidskriftsartikel (refereegranskat)abstract
- Adherence of bacterial pathogens to host tissues contributes to colonization and virulence and typically involves specific interactions between bacterial proteins called adhesins and cognate oligosaccharide (glycan) or protein motifs in the host that are used as receptors. A given pathogen may have multiple adhesins, each specific for a different set of receptors and, potentially, with different roles in infection and disease. This chapter provides strategies for identifying and analyzing host glycan receptors and the bacterial adhesins that exploit them as receptors, with particular reference to adherence of the gastric pathogen Helicobacter pylori.
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| 3. |
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| 4. |
- Bylund, Dan, et al.
(författare)
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Chromatographic alignment by warping and dynamic programming as a pre-processing tool for PARAFAC modelling of liquid chromatography-mass spectrometry data
- 2002
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Ingår i: Journal of Chromatography A. - 0021-9673. ; 961:2, s. 237-244
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Tidskriftsartikel (refereegranskat)abstract
- Solutes analysed with LC–MS are characterised by their retention times and mass spectra, and quantified by the intensities measured. This highly selective information can be extracted by multiway modelling. However, for full use and interpretability it is necessary that the assumptions made for the model are valid. For PARAFAC modelling, the assumption is a trilinear data structure. With LC–MS, several factors, e.g. non-linear detector response and ionisation suppression may introduce deviations from trilinearity. The single largest problem, however, is the retention time shifts not related to the true sample variations. In this paper, a time warping algorithm for alignment of LC–MS data in the chromatographic direction has been examined. Several refinements have been implemented and the features are demonstrated for both simulated and real data. With moderate time shifts present in the data, pre-processing with this algorithm yields approximately trilinear data for which reasonable models can be made.
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| 5. |
- Bylund, Dan, et al.
(författare)
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Peak purity assessment in liquid chromatography-mass spectrometry
- 2001
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Ingår i: Journal of Chromatography A. - 0021-9673. ; 915:1/2, s. 43-52
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Tidskriftsartikel (refereegranskat)abstract
- Fixed-size moving window evolving factor analysis and base peak chromatograms have been used for peak purity detection in data generated with LC–MS. The two methods were evaluated with both real and simulated data and were found to be fast and complementary to each other. When a possibly impure peak is detected, it is suggested that further information can be obtained from local principal component analysis modelling and comparative mass chromatogram plots.
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| 6. |
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| 7. |
- Danielsson, Rolf, et al.
(författare)
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Matched filtering with background suppression for improved quality of base peak chromatograms and mass spectra in liquid chromatography-mass spectrometry
- 2002
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Ingår i: Analytica Chimica Acta. - 0003-2670. ; 454:2, s. 167-184
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Tidskriftsartikel (refereegranskat)abstract
- A time domain filter that combines the properties of matched filtering and two-fold differentiation is presented. The filter coefficients are given by the second derivative of a Gaussian model peak, controlled by the setting of two parameters related to the chromatographic system. The fundamental characteristics of the filter were derived, and its applicability demonstrated for real liquid chromatography–mass spectrometry (LC–MS) data. The filter is primarily intended as a fast pre-processing step, for a mass chromatogram with 320 scans over 700 mass channels the computation time was 0.6 s on a standard PC. Base peak chromatograms with improved peak detection capability and mass spectra useful for compound identification were obtained with filtered data. The most significant effect of the described filter is background reduction due to the differentiation, which in combination with the matched filter can be performed with maintained or even improved signal-to-noise ratio.
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| 8. |
- Danielsson-Tham, Marie-Louise, et al.
(författare)
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Vad gjorde masken i köttfärsen?
- 2006
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Ingår i: Svensk veterinärtidning. - 0346-2250. ; 58, s. 31-31
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Tidskriftsartikel (refereegranskat)
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| 9. |
- Forsum, Urban, 1946-, et al.
(författare)
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Developments in the recent past - Immunology
- 2007
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Ingår i: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS). - : Wiley-Blackwell Publishing Inc.. - 0903-4641 .- 1600-0463. ; 115:5, s. 406-408
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 10. |
- Hagman, Monica, et al.
(författare)
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Mutagenicity from neutrophils after challenge with Helicobacter pylori and bile
- 1997
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Ingår i: European Journal of Surgery. - 1102-4151 .- 1741-9271. ; 163:10, s. 753-759
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To study some mechanisms involved in Helicobacter pylori (H. pylori)-induced gastric carcinogenesis.DESIGN: In vitro study.SETTING: Medical centre hospital, Sweden.INTERVENTIONS: Mutagenicity in Ames' test of neutrophils challenged for 2 hours or more by two different strains of H. pylori. One strain designated NCTC 11637 by the National College of Type Cultures activated neutrophils to an oxidative burst and producing vacuolating cytotoxin, the other strain C-7050 lacked these abilities. Mutagenicity was also studied with sterile human gall bladder bile alone added to neutrophils or in combination with both neutrophils and H. pylori.RESULTS: There was no increase in the number of revertants with the crude suspension or the supernatant of neutrophils challenged for 1 hour or less with H. pylori, bile, or the combination of both. However, in 5 out of 19 experiments there was significant mutagenicity after challenge of neutrophils for 2 hours or more with either strain of H. pylori, bile, or the combination of the two. The strongest mutagenicity was obtained after challenge over night (18 hours) with the combination of H. pylori and bile.CONCLUSION: Mutagenicity occurs when neutrophils are challenged with H. pylori and bile. Factors other than reactive oxygen metabolites seem to be responsible.
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