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- White, H, et al.
(författare)
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A certified plasmid reference material for the standardisation of BCR-ABL1 mRNA quantification by real time quantitative PCR.
- 2015
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Ingår i: Leukemia. - : Springer Science and Business Media LLC. - 1476-5551 .- 0887-6924. ; 29:2, s. 369-376
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Tidskriftsartikel (refereegranskat)abstract
- Serial quantification of BCR-ABL1 mRNA is an important therapeutic indicator in chronic myeloid leukemia, but there is substantial variation in results reported by different laboratories. To improve comparability, an internationally accepted plasmid certified reference material (CRM) was developed according to ISO Guide 34:2009. Fragments of BCR-ABL1 (e14a2 mRNA fusion), BCR and GUSB transcripts were amplified and cloned into pUC18 to yield plasmid pIRMM0099. Six different linearised plasmid solutions were produced with the following copy number concentrations, assigned by digital PCR, and expanded uncertainties: 1.08±0.13 × 10(6), 1.08±0.11 × 10(5), 1.03±0.10 × 10(4), 1.02±0.09 × 10(3), 1.04±0.10 × 10(2) and 10.0±1.5 copies/μL. The certification of the material for the number of specific DNA fragments per plasmid, copy number concentration of the plasmid solutions and the assessment of inter-unit heterogeneity and stability were performed according to ISO Guide 35:2006. Two suitability studies performed by 63 BCR-ABL1 testing laboratories demonstrated that this set of 6 plasmid CRMs can help to standardise the numbers of measured transcripts of e14a2 BCR-ABL1 and three control genes; ABL1, BCR and GUSB. The set of 6 plasmid CRMs is distributed worldwide by the Institute for Reference Materials and Measurements (Belgium) and its authorised distributors (http://irmm.jrc.ec.europa.eu; CRM code ERM-AD623a-f).Leukemia accepted article preview online, 18 July 2014; doi:10.1038/leu.2014.217.
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- Åström, Fredrik, et al.
(författare)
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Conceptualising dimensions of bibliometric assessment: From resource allocation systems to evaluative landscapes
- 2019
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Ingår i: Proceedings of the 17th Conference of the International Society for Scientometrics and Informetrics : ISSI 2019 with a Special STI Indicators Conference Track - ISSI 2019 with a Special STI Indicators Conference Track. - 9788833811185 ; , s. 1256-1261
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Konferensbidrag (refereegranskat)abstract
- The purpose of this paper is to discuss the conceptualisation of bibliometric analyses in terms of the levels on which they are performed, adding contextual factors to the dimension where the size of the unit being analysed is considered. Based on empirical investigations of resource allocation systems and research evaluation practices, as well as the previous literature conceptualising bibliometric analyses, a framework based on Whitley’s (2000) notion of research fields as ‘reputational work organisations’, is discussed. The results suggest adding a contextual ‘reputational dimension’ to the size-based dimension distinguishing between micro-, meso- and macro-level analyses. Furthermore, we propose that ‘evaluative landscapes’ (Brandter, 2017) might be a fruitful approach for further analysing how complex and multifaceted landscapes of research assessment affects the individual researcher.
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