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| 2. |
- Giampietro, C, et al.
(författare)
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The alternative splicing factor Nova2 regulates vascular development and lumen formation
- 2015
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Ingår i: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 6, s. 8479-
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Tidskriftsartikel (refereegranskat)abstract
- Vascular lumen formation is a fundamental step during angiogenesis; yet, the molecular mechanisms underlying this process are poorly understood. Recent studies have shown that neural and vascular systems share common anatomical, functional and molecular similarities. Here we show that the organization of endothelial lumen is controlled at the post-transcriptional level by the alternative splicing (AS) regulator Nova2, which was previously considered to be neural cell-specific. Nova2 is expressed during angiogenesis and its depletion disrupts vascular lumen formation in vivo. Similarly, Nova2 depletion in cultured endothelial cells (ECs) impairs the apical distribution and the downstream signalling of the Par polarity complex, resulting in altered EC polarity, a process required for vascular lumen formation. These defects are linked to AS changes of Nova2 target exons affecting the Par complex and its regulators. Collectively, our results reveal that Nova2 functions as an AS regulator in angiogenesis and is a novel member of the ‘angioneurins’ family.
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| 3. |
- Cataldi, A., et al.
(författare)
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A comparison between micro- and nanocellulose-filled composite adhesives for oil paintings restoration
- 2015
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Ingår i: Nanocomposites. - : Taylor and Francis Inc.. - 2055-0332 .- 2055-0324. ; 1:4, s. 195-203
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Tidskriftsartikel (refereegranskat)abstract
- Cellulose nanocrystals (CNC) and microcrystals (CMC) were selected as reinforcing fillers for poly(2-ethyl-2-oxazoline), a water-soluble thermoplastic adhesive widely used in the restoration of oil paintings. Thin composite films containing 5, 10, and 30 wt% of CNC or CMC were produced by solution mixing and casting. UV-vis spectroscopy showed how CNC preserved the adhesive transparency even at the highest CNC content, while for CMC, a progressive loss of transparency was observed. Thermal analysis evidenced a progressive increase of the glass transition temperature of the polymer matrix induced by CNC, while no effects were observed for CMC. Both micro- and nanocellulose were able to improve the elastic modulus and reduce the thermal expansion coefficient and creep compliance of the adhesive, with effects more pronounced for CNC nanoparticles. Finally, single-lap shear test on bonded ancient oil painting substrates confirmed the improved dimensional stability of the joint imparted by CNC in the adhesive under both quasi-static and creep conditions.
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| 4. |
- Cataldi, A., et al.
(författare)
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Polymer composite with micro- and nanocellulose for artwork protection and restoration
- 2014
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Ingår i: 16th European Conference on Composite Materials, ECCM 2014. - 9780000000002
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Konferensbidrag (refereegranskat)abstract
- Micro- and nanocomposites were prepared using Aquazol® 500 as a polymeric matrix and a microcrystalline cellulose (MCC), and suspensions of cellulose nanocrystals (CNC), as reinforcing agents. After a preliminary thermo-mechanical characterization that highlighted a stabilizing effect due to the micro- and nanofiller introduction, with an increase of the elastic modulus and a decrease of the thermal expansion coefficient and the creep compliance [1], these materials were applied as canvas lining adhesives. Single-lap shear tests both in quasi-static and creep conditions confirmed the dimensional stability provided by cellulose micro- and nanoparticles, with an important reduction of the adhesives compliance proportional to the filler content. Interestingly, MCC and CNC introduction did not impair the fracture behavior of the neat matrix.
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| 5. |
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| 6. |
- Deflorian, F., et al.
(författare)
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Stress corrosion cracking (SCC) failure in marine areas of fixed guards for climbing
- 2015
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Ingår i: Corrosion Engineering, Science and Technology. - 1478-422X .- 1743-2782. ; 50:6, s. 462-466
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Tidskriftsartikel (refereegranskat)abstract
- This work studies the particular mechanism of environmental stress corrosion cracking (SCC) that has been described to interest stainless steel products, like climbing anchors, installed in sea areas. The failure analysis of several broken anchors was carried out. The samples were collected in different parts of the world, always from climbing structures close to the sea. The analysis confirmed the stress corrosion mechanism of degradation, giving also important information about the specific environments causing the metal fracture. These results are in agreement with a few previous works about this subject and are in the frame of the larger topic of SCC of austenitic stainless steel at room temperature. Moreover, some corrosion tests were carried out on stainless steel samples simulating the operation conditions, after contamination with electrolytes at different concentration. The tests are performed in order to better understand the degradation mechanism and to evaluate the influence of some environmental parameters over the susceptibility to SCC. With these experimental data, a possible interpretation model has been proposed together with some reasonable solutions for the material selection process, considering the problem's characteristics and the multiple alternatives available nowadays for climbing materials.
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| 7. |
- Ecco, L. G., et al.
(författare)
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EIS and in situ AFM study of barrier property and stability of waterborne and solventborne clear coats
- 2014
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Ingår i: Progress in organic coatings. - : Elsevier BV. - 0300-9440 .- 1873-331X. ; 77:3, s. 600-608
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Tidskriftsartikel (refereegranskat)abstract
- Various processes can occur when paints are in contact with moisture, such as ingress of water and aggressive ions into the coating. As a consequence, the microstructure and properties of the paints can be affected. The present study combines electrochemical impedance spectroscopy (EIS) and in situ atomic force microscopy (AFM) to investigate the barrier property of waterborne and solventborne coatings on mild steel, paying particular attention to the occurrences in the first 24 h after contact between the coating surface and electrolyte. The sequential in situ AFM images revealed that changes on the order of hundreds of nanometres at the coating surface have occurred shortly after the exposure to the electrolytes. EIS observations for the clear waterborne alkyd coating revealed a rise in the |Z|0.015Hz and a decrease in the coating capacitance after a few hours of exposure. Evidences that water uptake caused swelling of the coating and promoted the closure/blockage of pores were given by means of in situ AFM. The solventborne alkyd emulsion has demonstrated lower reactivity to the presence of the electrolyte and a correlation between the coating resistance and defects/pores evolution is suggested.
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| 8. |
- Fedel, M., et al.
(författare)
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Effect of the synthesis parameters of in situ grown Mg-Al LDHs on the filiform corrosion susceptibility of painted AA5005
- 2021
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Ingår i: Electrochimica Acta. - : Elsevier. - 0013-4686 .- 1873-3859. ; 381
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Tidskriftsartikel (refereegranskat)abstract
- Novel layered double hydroxides (LDHs) based coatings developed in-situ on aluminum alloys are recognized to provide the substrate with improved corrosion protection. LDH layers have gained prominent attention due to their barrier properties and ions exchange capability, together with compositional flexibility and low environmental impact. In this work, diverse MgAl LDHs layers are developed on AA5005 as a surface conversion treatment prior to applying an acrylic clearcoat. The work aims to assess the potential LDH layers of to improve the filiform corrosion (FFC) resistance of the AA500 substrate. The effect of the synthesis time and the presence of urea on the FFC susceptibility are investigated. The performance of the different synthesis conditions was compared and shown to be more effective to increase FFC resistance when well-defined crystals are formed. The findings suggest that MgAl-LDHs mitigate the extent of filiform corrosion of acrylic paint coated AA5005. The FFC inhibition was found to be qualitatively proportional to the pitting potential measured over the LDHs conversion layers.
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| 9. |
- Högberg, Carl, et al.
(författare)
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Farnesyl pyrophosphate is an endogenous antagonist to ADP-stimulated P2Y12 receptor-mediated platelet aggregation.
- 2012
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Ingår i: Thrombosis and Haemostasis. - 0340-6245. ; 108:1, s. 119-132
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Tidskriftsartikel (refereegranskat)abstract
- Farnesyl pyrophosphate (FPP) is an intermediate in cholesterol biosynthesis, and it has also been reported to activate platelet LPA (lysophosphatidic acid) receptors. The aim of this study was to investigate the role of extracellular FPP in platelet aggregation. Human platelets were studied with light transmission aggregometry, flow cytometry and [35S]GTPγS binding assays. As shown previously, FPP could potentiate LPA-stimulated shape change. Surprisingly, FPP also acted as a selective insurmountable antagonist to ADP-induced platelet aggregation. FPP inhibited ADP-induced expression of P-selectin and the activated glycoprotein (Gp)IIb/IIIa receptor. FPP blocked ADP-induced inhibition of cAMP accumulation and [35S]GTPγS binding in platelets. In Chinese hamster ovary cells expressing the P2Y12 receptor, FPP caused a rightward shift of the [35S]GTPγS binding curve. In Sf9 insect cells expressing the human P2Y12 receptor, FPP showed a concentration-dependent, although incomplete inhibition of [3H]PSB-0413 binding. Docking of FPP in a P2Y12 receptor model revealed molecular similarities with ADP and a good fit into the binding pocket for ADP. In conclusion, FPP is an insurmountable antagonist of ADP-induced platelet aggregation mediated by the P2Y12 receptor. It could be an endogenous antithrombotic factor modulating the strong platelet aggregatory effects of ADP in a manner similar to the use of clopidogrel, prasugrel or ticagrelor in the treatment of ischaemic heart disease.
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| 10. |
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