| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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| 3. |
- Feng, Zhenhua, et al.
(författare)
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Multicore-Fiber-Enabled WSDM Optical Access Network With Centralized Carrier Delivery and RSOA-Based Adaptive Modulation
- 2015
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Ingår i: IEEE PHOTONICS JOURNAL. - : Institute of Electrical and Electronics Engineers (IEEE). - 1943-0655. ; 7:4
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Tidskriftsartikel (refereegranskat)abstract
- We proposed and experimentally demonstrated a wavelength-space division multiplexing (WSDM) optical access network architecture with centralized optical carrier delivery utilizing multicore fibers (MCFs) and adaptive modulation based on reflective semiconductor amplifier (RSOA). In our experiment, five of the outer cores are used for undirectional downstream (DS) transmission only, whereas the remaining outer core is utilized as a dedicated channel to transmit upstream (US) signals. Optical carriers for US are delivered from the optical line terminal (OLT) to the optical network unit (ONU) via the inner core and then transmitted back to the OLT after amplification and modulation by the RSOA in the colorless ONU side. The mobile backhaul (MB) service is also supported by the inner core. Wavelengths used in US transmission should be different from that of the MB in order to avoid the Rayleigh backscattering effect in bidirectional transmission. With quadrature phase-shift keying-orthogonal frequency-division multiplexing (QPSK-OFDM) modulation format, the aggregation DS capacity reaches 250 Gb/s using five outer cores and ten wavelengths, and it can be further scaled to 1 Tb/s using 20 wavelengths modulated with 16 QAM-OFDM. For US transmission, 2.5 Gb/s QPSK-OFDM transmission can be achieved just using a low-bandwidth RSOA, and adaptive modulation is applied to the RSOA to further enhance the US data rate to 3.12 Gb/s. As an emulation of high-speed MB transmission, 48 Gb/s inphase and quadrature (IQ) modulated popularization division multiplexing (PDM)-QPSK signal is transmitted in the inner core of MCF and coherently detected in the OLT side. Both DS and US optical signals exhibit acceptable performance with sufficient power budget.
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| 4. |
- Deng, Bin, et al.
(författare)
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Ketamine inhibits TNF-α-induced cecal damage by enhancing RIP1 ubiquitination to attenuate lethal SIRS
- 2022
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Ingår i: Cell death discovery. - : Springer Science and Business Media LLC. - 2058-7716. ; 8:1
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Tidskriftsartikel (refereegranskat)abstract
- Systemic inflammatory response syndrome (SIRS) is a sepsis-associated inflammatory state and a self-defense mechanism against specific and nonspecific stimuli. Ketamine influences many key processes that are altered during sepsis. However, the underlying mechanisms remain incompletely understood. In this study, TNF-α-treated mice, as well as HT-29 and L929 cell models, were applied to characterize TNF-α-induced systemic and local cecal tissue inflammatory responses. Behavioral, biochemical, histological, and molecular biological approaches were applied to illustrate the related processes. Mice with TNF-α-induced SIRS showed systemic and local cecal tissue inflammatory responses, as indicated by increased levels of high mobility group box 1 protein (HMGB1), chemokines (C-X-C motif) ligand 10 (CXCL10), interleukin-6 (IL-6), and IL-10, as well as high mortality. Ketamine pretreatment alleviated death rates, symptoms, and the production of inflammatory cytokines induced by TNF-α in mice. Moreover, ketamine also protected the mice from TNF-α-induced cecal damage by suppressing the phosphorylation of receptor-interacting serine/threonine-protein kinase 3 (RIP3) and mixed lineage kinase domain-like protein (MLKL). In addition, our results showed that ketamine efficiently inhibited TNF-α-induced necroptosis in HT-29 and L929 cells. Furthermore, we explored the mechanism using different L929 cell lines. The results displayed that ketamine inhibited TNF-α-induced necroptosis by enhancing RIP1 ubiquitination and reducing the RIP1-RIP3 and RIP3-MLKL interactions, as well as the formation of necrosomes. Thus, our study may provide a new theoretical and experimental basis for treating diseases characterized by SIRS-associated inflammatory factor storms. Moreover, our exploration may provide potential molecular mechanisms and targets for therapeutic intervention and clinical application of ketamine.
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| 5. |
- Feng, Z., et al.
(författare)
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A simplified adaptive modulation scheme for RSOA based DDO-OFDM system using CAZAC precoding
- 2016
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Ingår i: 2016 Optical Fiber Communications Conference and Exhibition, OFC 2016. - Washington, D.C. : Institute of Electrical and Electronics Engineers (IEEE).
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Konferensbidrag (refereegranskat)abstract
- We experimentally demonstrated a RSOA based DDO-OFDM system using CAZAC precoding. Adaptive modulation has been simply and efficiently performed after CAZAC precoding to improve the sensitivity and overall data rate thanks to the equalized SNR.
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| 6. |
- Feng, Zhenhua, et al.
(författare)
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Digital Domain Power Division Multiplexing DDO-OFDM Transmission with Successive Interference Cancellation
- 2016
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Ingår i: 2016 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO). - Washington, D.C. : IEEE conference proceedings.
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Konferensbidrag (refereegranskat)abstract
- Two independent 2.5-Gb/s DDO-OFDM signals are simultaneously transmitted over 25km SMF using digital domain power division multiplexing and successive interference cancellation. With optimized power division ratio and enhanced SD-FEC, the spectral efficiency can be doubled.
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| 7. |
- Feng, Zhenhua, et al.
(författare)
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Performance-Enhanced Direct Detection Optical OFDM Transmission With CAZAC Equalization
- 2015
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Ingår i: IEEE Photonics Technology Letters. - 1041-1135 .- 1941-0174. ; 27:14, s. 1507-1510
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Tidskriftsartikel (refereegranskat)abstract
- Direct detection optical orthogonal frequency division multiplexing (DDO-OFDM) transmission with constant amplitude zero autocorrelation (CAZAC) sequence equalization is proposed and experimentally demonstrated. Simulation results show that more than 2-dB peak-to-average power ratio (PAPR) reduction can be realized using CAZAC equalization, and 50-km standard single mode fiber (SSMF) transmission of 4.11-Gb/s QPSK-OFDM can be achieved with bit-error rate (BER) under forward error correction limit. Transmission performance of QPSK-based DDO-OFDM system is analyzed in both OB2B configuration and fiber link with and without CAZAC equalization. More than 2.5-dB optical receiver sensitivity improvements can be obtained thanks to the PAPR reduction enjoyed by CAZAC equalization. Signal-to-noise ratio for every subcarrier derived from error vector magnitude is estimated and its flatness is confirmed to be much improved with CAZAC equalization. The performance improvements brought by CAZAC equalization can be extended to other modulation formats, and 8.22-Gb/s 16-quadratic-amplitude modulation-OFDM signals transmission using CAZAC equalization is demonstrated with over 1.5 dB enhancement in receiver sensitivity.
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| 8. |
- Feng, Z., et al.
(författare)
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Power efficient optical OFDM transmission with phase modulation and direct detection
- 2016
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Ingår i: 2016 21st OptoElectronics and Communications Conference, OECC 2016 - Held Jointly with 2016 International Conference on Photonics in Switching, PS 2016. - : IEEE conference proceedings. - 9784885523052
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Konferensbidrag (refereegranskat)abstract
- Optical OFDM transmission with phase modulation and direct detection (PMDD) is verified by theoretical derivation and simulation. 26.12-Gb/s PMDD 16QAM-OFDM achieves comparable performance to single sideband IMDD 16QAM-OFDM with half of the optical modulation index.
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| 9. |
- Feng, Zhenhua, et al.
(författare)
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SNR equalized optical direct-detected OFDM transmission with CAZAC equalization
- 2015
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Ingår i: CLEO. - : Optica Publishing Group.
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Konferensbidrag (refereegranskat)abstract
- 50Km SSMF optical direct-detected OFDM transmission with Constant Amplitude Zero Auto Correlation Sequence (CAZAC) equalization is experimentally demonstrated with over 15dB power budget. 2.5dB enhancement in sensitivity has been achieved simultaneously with 3dB PAPR suppression.
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| 10. |
- Feng, Z., et al.
(författare)
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Spectrally overlaid DDO-OFDM transmission enabled by optical power division multiplexing
- 2017
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Ingår i: ICOCN 2016 - 2016 15th International Conference on Optical Communications and Networks. - : IEEE. - 9781509034918
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Konferensbidrag (refereegranskat)abstract
- Two 8.3-Gb/s spectrally overlaid DDO-OFDM signals are successfully transmitted along 50-km SMF using optical power division multiplexing and received by a successive interference cancellation (SIC) receiver. Spectral efficiency is doubled with optimized optical modulation index and optical power division ratio.
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