| 1. |
- Bjerselius, Rickard, et al.
(författare)
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Male goldfish reproductive behaviour and physiology are severely affected by exogenous exposure to 17 beta-estradiol
- 2001
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Ingår i: Aquatic Toxicology. - 0166-445X .- 1879-1514. ; 53:2, s. 139-152
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Tidskriftsartikel (refereegranskat)abstract
- Mature male goldfish were exposed to different concentrations of the natural hormone 17 beta -estradiol (El). Two methods of exposure were employed, via ingestion at 0, 1, 10 and 100 ug/g food and via the water at 0, 1 and 10 mug/l. The fish were exposed fur 74-28 days during the spawning period. The males were then paired with an artificially induced, spawning female and their sexual behaviour was observed during a 15 min period. The physiological status of the fish was also examined with respect to GSI. presence of milt and spawning tubercles and the blood plasma concentration of E-2. Despite the relatively short exposure period. exposure to physiological levels of E-2 was shown to severely affect the male goldfish reproductive behaviour and physiology. In conclusion, the results from this study and the ability to interpret the effects on this well-studied species, show that the effects of E-2, and possibly other estrogenic EDCs have severe effects at several vital levels of male goldfish reproduction. The results also suggests that the hormone E-2 can act as an endocrine disrupting chemical (EDC) in the environment.
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| 2. |
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| 4. |
- Lee-Manion, M., et al.
(författare)
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In Vitro Antioxidant Activity and Antigenotoxic Effects of Avenanthramides and Related Compounds
- 2009
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Ingår i: Journal of Agricultural and Food Chemistry. - : American Chemical Society (ACS). - 0021-8561 .- 1520-5118. ; 57:22, s. 10619-10624
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Tidskriftsartikel (refereegranskat)abstract
- Avenanthramides are substituted N-cinnamoylanthranilic acids, with hydroxycinnamic acid and anthranilic acid moieties. These alkaloid phenols, which are unique to oats, may confer health benefits via antioxidant or other mechanisms. Synthetic avenanthramides, hydroxycinnamic acids, Tranilast, and ascorbic acid were evaluated for antioxidant activity using two assays, DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant potential), and for antigenotoxicity using the Comet assay with stressed human adenocarcinoma colon cells. Of all the compounds tested, N-(3',4'-dihydroxy-(E)-cinnamoyl)-5-hydroxyanthranilic acid (2c), an abundant oat avenanthramide, generally had the highest activity in all three assays. The drug Tranilast showed antigenotoxic effects, but not antioxidant activity, suggesting that antigenotoxicity is not dependent on antioxidant effects. Overall, results show that avenanthramides exert antioxidant and antigenotoxic activities that are comparable to those of ascorbic acid and which have the potential to exert beneficial physiological effects.
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| 5. |
- Nowak-Sliwinska, Patrycja, et al.
(författare)
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Consensus guidelines for the use and interpretation of angiogenesis assays
- 2018
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Ingår i: Angiogenesis. - : Springer. - 0969-6970 .- 1573-7209. ; 21:3, s. 425-532
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Forskningsöversikt (refereegranskat)abstract
- The formation of new blood vessels, or angiogenesis, is a complex process that plays important roles in growth and development, tissue and organ regeneration, as well as numerous pathological conditions. Angiogenesis undergoes multiple discrete steps that can be individually evaluated and quantified by a large number of bioassays. These independent assessments hold advantages but also have limitations. This article describes in vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis and highlights critical aspects that are relevant for their execution and proper interpretation. As such, this collaborative work is the first edition of consensus guidelines on angiogenesis bioassays to serve for current and future reference.
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| 6. |
- Bjerselius, R, et al.
(författare)
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Male goldfish reproductive and physiology are severely affected by exogenous exposure to 17b-estradiol
- 2001
-
Ingår i: Aquatic Toxicology. - 0166-445X .- 1879-1514. ; 53:2, s. 139-152
-
Tidskriftsartikel (refereegranskat)abstract
- Mature male goldfish were exposed to different concentrations of the natural hormone 17beta-estradiol (E2). Two methods of exposure were employed, via ingestion at 0, 1, 10 and 100 microg/g food and via the water at 0, 1 and 10 microg/l. The fish were exposed for 24-28 days during the spawning period. The males were then paired with an artificially induced, spawning female and their sexual behaviour was observed during a 15 min period. The physiological status of the fish was also examined with respect to GSI, presence of milt and spawning tubercles and the blood plasma concentration of E2. Despite the relatively short exposure period, exposure to physiological levels of E2 was shown to severely affect the male goldfish reproductive behaviour and physiology. In conclusion, the results from this study and the ability to interpret the effects on this well-studied species, show that the effects of E2, and possibly other estrogenic EDCs, have severe effects at several vital levels of male goldfish reproduction. The results also suggests that the hormone E2 can act as an endocrine disrupting chemical (EDC) in the environment.PMID: 11311390 [PubMed - indexed for MEDLINE]
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