| 1. |
- Dixit, Pooja, et al.
(författare)
-
Lytic Polysaccharide Monooxygenases : Production and Applications
- 2024
-
Ingår i: <em>Biomass Hydrolyzing Enzymes: Basics, Advancements, and Applications</em>. - : CRC Press. ; , s. 86-97
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- The discovery of lytic polysaccharide monooxygenases (LPMOs) as a cellulase component has brought a significant revolution to the enzymatic degradation of lignocellulosic biomass. LPMOs are powerful oxidative enzymes which can cleave glycosidic bonds in polysaccharides, such as starch, cellulose, xylan and chitin. The mechanism of action and analysis of LPMOs is complex due to which its discovery has been delayed even though its other counterparts as cellulase components have been known for some time. Their significance in biomass hydrolysis is presented in this chapter along with details of the mechanism of action and determination methods associated with complexities and future avenues. The addition of LPMOs can further reduce cellulase cost in enzyme cocktails of enzyme manufacturers, which has increased the rate of hydrolysis by more than twofold.
|
|
| 2. |
- Gurrani, Swapnil, et al.
(författare)
-
Biomass Hydrolyzing Enzymes
- 2024
-
Ingår i: <em>Biomass Hydrolyzing Enzymes: Basics, Advancements, and Applications</em>. - : CRC Press. ; , s. 3-13
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Lignocellulosic biomass is the most abundant, ubiquitous polymer on Earth available to benefit mankind. Biomass biorefinery has taken center stage in the world’s economy and is becoming diverse with time. With biorefinery, cascades of products can be obtained from biomass rather than a single product. It is a complex structure that consists of three polymers as cellulose, hemicellulose and lignin, linked to each other in a compact intricate manner making it difficult to be attacked by microorganisms. Still, many microorganisms have evolved strategies to consume this abundant material as an energy source. Degradation of lignocellulosic biomass is not only a survival strategy for these microbes, but its destruction also plays a pivotal role in carbon recycling of Earth through the fixation of photosynthetically fixed carbon present in plant biomass. Mankind has explored the capacity of microorganisms to produce polymer-degrading enzymes to deconstruct its basic components. Biomass-degrading enzymes have attracted researchers worldwide as this is the most sustainable way to obtain fermentable sugars from this most abundant biomass. Synergism among enzymes as well as their various components are presented along with challenges of biomass hydrolysis and probable solutions. © 2024 selection and editorial matter, Reeta Rani Singhania, Anil Kumar Patel, Héctor A. Ruiz, Ashok Pandey; individual chapters, the contributors.
|
|
| 3. |
- Martin, Carlos, et al.
(författare)
-
Hydrothermal Pretreatment of Lignocellulosic Feedstocks to Facilitate Biochemical Conversion
- 2022
-
Ingår i: Frontiers in Bioengineering and Biotechnology. - : Frontiers Media S.A.. - 2296-4185. ; 10
-
Forskningsöversikt (refereegranskat)abstract
- Biochemical conversion of lignocellulosic feedstocks to advanced biofuels and other bio-based commodities typically includes physical diminution, hydrothermal pretreatment, enzymatic saccharification, and valorization of sugars and hydrolysis lignin. This approach is also known as a sugar-platform process. The goal of the pretreatment is to facilitate the ensuing enzymatic saccharification of cellulose, which is otherwise impractical due to the recalcitrance of lignocellulosic feedstocks. This review focuses on hydrothermal pretreatment in comparison to alternative pretreatment methods, biomass properties and recalcitrance, reaction conditions and chemistry of hydrothermal pretreatment, methodology for characterization of pretreatment processes and pretreated materials, and how pretreatment affects subsequent process steps, such as enzymatic saccharification and microbial fermentation. Biochemical conversion based on hydrothermal pretreatment of lignocellulosic feedstocks has emerged as a technology of high industrial relevance and as an area where advances in modern industrial biotechnology become useful for reducing environmental problems and the dependence on fossil resources.
|
|
| 4. |
- Rani Singhania, Reeta, et al.
(författare)
-
Role and significance of lytic polysaccharide monooxygenases (LPMOs) in lignocellulose deconstruction
- 2021
-
Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 335
-
Forskningsöversikt (refereegranskat)abstract
- Lytic polysaccharide monooxygenases (LPMOs) emerged a decade ago and have been described as biomass deconstruction boosters as they play an extremely important role in unravelling the enzymatic biomass hydrolysis scheme. These are oxidative enzymes requiring partners to donate electrons during catalytic action on cellulose backbone. Commercial cellulase preparations are mostly from the robust fungal sources, hence LPMOs from fungi (AA9) have been discussed. Characterisation of LPMOs suffers due to multiple complications which has been discussed and challenges in detection of LPMOs in secretomes has also been highlighted. This review focuses on the significance of LPMOs on biomass hydrolysis due to which it has become a key component of cellulolytic cocktail available commercially for biomass deconstruction and its routine analysis challenge has also been discussed. It has also outlined a few key points that help in expressing catalytic active recombinant AA9 LPMOs.
|
|
