| 1. |
- Dobielewski, Mikolaj, et al.
(författare)
-
All-in-one sampler for one-step dried blood spot sample collection
- 2021
-
Ingår i: Proceedings MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. - : Chemical and Biological Microsystems Society. ; , s. 675-676
-
Konferensbidrag (refereegranskat)abstract
- We present the first fully integrated device for one-step Dried Blood Spot (DBS) sampling. We maximize the sample aspiration efficiency by combining the function of skin lancing and sample aspiration in one component, thus minimizing the required incision size and the related pain. Our volunteer study shows reliable collection of 1 µL of blood in under one minute, while significantly lowering the related pain and discomfort, compared to finger-prick sample collection. The collected samples show quality on par with finger-prick capillary blood samples.
|
|
| 2. |
- Dobielewski, Mikolaj, et al.
(författare)
-
Blood Cell Quantification on Dry Blood Samples - Towards Patient-CentricComplete Blood Counts (CBC)
- 2022
-
Ingår i: Bioanalysis. - : Future Science Ltd. - 1757-6180 .- 1757-6199.
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Performing complete blood counts (CBC) from patients´ homes could have atransformative impact on e-based healthcare. Blood microsampling and sample drying are enablingelements for patient-centric healthcare. The aim of this study is to investigate the potential of dry bloodsamples for image-based cell quantification of red and white blood cells. Methods: A manual samplepreparation method is developed and tested for image-based red and white blood cell counting. Resultsand Conclusion: Dry blood samples enable image-based cell counting of red and white blood cells with agood correlation to gold standard hematology analyzer data (avg. CV < 6.5%, R2 > 0.8), and resolve thebasic morphology of white blood cell nuclei. The presented proof-of-principle study is a first step towardpatient-centric CBCs.
|
|
| 3. |
- Garciá-Carmona, L., et al.
(författare)
-
Biohybrid systems for environmental intelligence on living plants : WatchPlant project
- 2021
-
Ingår i: GoodIT 2021 - Proceedings of the 2021 Conference on Information Technology for Social Good. - New York, NY, USA : Association for Computing Machinery (ACM). ; , s. 210-215
-
Konferensbidrag (refereegranskat)abstract
- New challenges such as climate change and sustainability arise in society influencing not only environmental issues but human's health directly. To face these new challenges IT technologies and their application to environmental intelligent monitoring become into a powerful tool to set new policies and blueprints to contribute to social good. In the new H2020 project, WatchPlant will provide new tools for environmental intelligence monitoring by the use of plants as "well-being"sensors of the environment they inhabit. This will be possible by equipping plants with a net of communicated wireless self-powered sensors, coupled with artificial intelligence (AI) to become plants into "biohybrid organisms"to test exposure-effects links between plant and the environment. It will become plants into a new tool to be aware of the environment status in a very early stage towards in-situ monitoring. Additionally, the system is devoted to be sustainable and energy-efficient thanks to the use of clean energy sources such as solar cells and a enzymatic biofuel cell (BFC) together with its self-deployment, self-awareness, adaptation, artificial evolution and the AI capabilities. In this concept paper, WatchPlant will envision how to face this challenge by joining interdisciplinary efforts to access the plant sap for energy harvesting and sensing purposes and become plants into "biohybrid organisms"to benefit social good in terms of environmental monitoring in urban scenarios.
|
|
| 4. |
- Ribet, Federico, et al.
(författare)
-
Microneedle Patch for Painless Intradermal Collection of Interstitial Fluid Enabling Multianalyte Measurement of Small Molecules, SARS‐CoV‐2 Antibodies, and Protein Profiling
- 2023
-
Ingår i: Advanced Healthcare Materials. - : Wiley. - 2192-2640 .- 2192-2659. ; 12:13
-
Tidskriftsartikel (refereegranskat)abstract
- Blood sampling is a common practice to monitor health, but it entails a series of drawbacks for patients including pain and discomfort. Thus, there is a demand for more convenient ways to obtain samples. Modern analytical techniques enable monitoring of multiple bioanalytes in smaller samples, opening possibilities for new matrices, and microsampling technologies to be adopted. Interstitial fluid (ISF) is an attractive alternative matrix that shows good correlation with plasma concentration dynamics for several analytes and can be sampled in a minimally invasive and painless manner from the skin at the point-of-care. However, there is currently a lack of sampling devices compatible with clinical translation. Here, to tackle state-of-the-art limitations, a cost-effective and compact single-microneedle-based device designed to painlessly collect precisely 1.1 µL of dermal ISF within minutes is presented. The fluid is volume-metered, dried, and stably stored into analytical-grade paper within the microfluidic device. The obtained sample can be mailed to a laboratory, quantitatively analyzed, and provide molecular insights comparable to blood testing. In a human study, the possibility to monitor various classes of molecular analytes is demonstrated in ISF microsamples, including caffeine, hundreds of proteins, and SARS-CoV-2 antibodies, some being detected in ISF for the first time.
|
|
| 5. |
- Ribet, Federico, et al.
(författare)
-
Minimally invasive and volume-metered extraction of interstitial fluid:bloodless point-of-care sampling for bioanalyte detection
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Sampling of biological fluids is fundamental in health monitoring and, currently, blood analysisis the standard practice. However, blood sampling entails a series of drawbacks including pain,discomfort, and risk of infections. Interstitial fluid, having a good correlation with blood concentrationdynamics for several analytes, is a promising alternative monitoring matrix because it can be sampled ina minimally invasively manner from the skin, without the need for the more invasive and painfulextraction methods used for blood. Currently, there are no simple devices available to sample and storeknown amounts of interstitial fluid. In this work, a painless microneedle-based sampling device designedto extract 1 μL of fluid from the dermis was realized. The sampled liquid is metered and stored in a papermatrix embedded in a microfluidic chip. The sample can then be analyzed using state-of-the-art tools,such as mass spectrometry (LC-MS/MS).
|
|
