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| 2. |
- Jung, Christian, et al.
(författare)
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A comparison of very old patients admitted to intensive care unit after acute versus elective surgery or intervention
- 2019
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Ingår i: Journal of critical care. - : W B SAUNDERS CO-ELSEVIER INC. - 0883-9441 .- 1557-8615. ; 52, s. 141-148
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Tidskriftsartikel (refereegranskat)abstract
- Background: We aimed to evaluate differences in outcome between patients admitted to intensive care unit (ICU) after elective versus acute surgery in a multinational cohort of very old patients (80 years; VIP). Predictors of mortality, with special emphasis on frailty, were assessed.Methods: In total, 5063 VIPs were induded in this analysis, 922 were admitted after elective surgery or intervention, 4141 acutely, with 402 after acute surgery. Differences were calculated using Mann-Whitney-U test and Wilcoxon test. Univariate and multivariable logistic regression were used to assess associations with mortality.Results: Compared patients admitted after acute surgery, patients admitted after elective surgery suffered less often from frailty as defined as CFS (28% vs 46%; p < 0.001), evidenced lower SOFA scores (4 +/- 5 vs 7 +/- 7; p < 0.001). Presence of frailty (CFS >4) was associated with significantly increased mortality both in elective surgery patients (7% vs 12%; p = 0.01), in acute surgery (7% vs 12%; p = 0.02).Conclusions: VIPs admitted to ICU after elective surgery evidenced favorable outcome over patients after acute surgery even after correction for relevant confounders. Frailty might be used to guide clinicians in risk stratification in both patients admitted after elective and acute surgery.
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| 3. |
- Arnqvist, Göran, et al.
(författare)
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Genetic architecture of metabolic rate : environment specific epistasis between mitochondrial and nuclear genes in an insect
- 2010
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Ingår i: Evolution. - : Wiley. - 0014-3820 .- 1558-5646. ; 64:12, s. 3354-3363
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Tidskriftsartikel (refereegranskat)abstract
- The extent to which mitochondrial DNA (mtDNA) variation is involved in adaptive evolutionary change is currently being reevaluated. In particular, emerging evidence suggests that mtDNA genes coevolve with the nuclear genes with which they interact to form the energy producing enzyme complexes in the mitochondria. This suggests that intergenomic epistasis between mitochondrial and nuclear genes may affect whole-organism metabolic phenotypes. Here, we use crossed combinations of mitochondrial and nuclear lineages of the seed beetle Callosobruchus maculatus and assay metabolic rate under two different temperature regimes. Metabolic rate was affected by an interaction between the mitochondrial and nuclear lineages and the temperature regime. Sequence data suggests that mitochondrial genetic variation has a role in determining the outcome of this interaction. Our genetic dissection of metabolic rate reveals a high level of complexity, encompassing genetic interactions over two genomes, and genotype x genotype x environment interactions. The evolutionary implications of these results are twofold. First, because metabolic rate is at the root of life histories, our results provide insights into the complexity of life-history evolution in general, and thermal adaptation in particular. Second, our results suggest a mechanism that could contribute to the maintenance of nonneutral mtDNA polymorphism.
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| 5. |
- Dowling, Tom
(författare)
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The drumlin problem : streamlined subglacial bedforms in southern Sweden
- 2016
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis investigates stream-lined subglacial bedforms (often referred to as drumlins) in southern Sweden. The broad aim of this is to contribute to the solution of the ‘drumlin problem’. The term drumlin has come to be applied to a wide range of features whose internal architecture (core) and overall morphology are seen to vary greatly. This range in characteristics is in part responsible for the various competing theories of drumlin formation, each different type of core and morphology generating a new idea for how it came about. Here the new Swedish national height model, a high resolution LiDAR derived digital elevation model, in combination with detailed sedimentological work is used to characterise streamlined terrain in southern Sweden and investigate the formation processes associated with it. The findings of this are that drumlinoids in southern Sweden are predominantly rock cored. Soft cored features tend to be significantly longer than rock cored features. In general, drumlinoids in southern Sweden are located at the lower end of the size spectrum in terms of global streamlined sub-glacial features. Additionally it has been found that drumlinoids can form rapidly at glacial margins as well as within the main body of ice sheets. And finally, the most important contextual geological factor in drumlinoid parameter (morphology) formation appears to be drift depth/properties. The bedrock type beneath a feature and the hydrological system as recorded in eskers do play a role, but the exact nature of this is not certain and the correlations are difficult to analyse. In addition to these findings a generalised conceptual model of drumlinoid formation is proposed and a discussion of the possible ways in which physical processes influence said formation is offered. It is suggested that chaotic behaviour and the role of scale might be useful to consider and that whilst it is something of semantic point, the use of the term drumlinoid is deliberate and important. This is because due to equifinality there are many landforms that researchers can split into different categories, e.g. rock drumlins, clone drumlins, emergent drumlins, downwards emergent drumlins or obstacle drumlins. These are all valid divisions as there are different physical processes involved in their formation. However these processes and the final landforms that result from them are all part of the sub-glacial continuum and so at one level must be considered part of the same family.
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| 7. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 8. |
- Möller, Per, et al.
(författare)
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The importance of thermal boundary transitions on glacial geomorphology; mapping of ribbed/hummocky moraine and streamlined terrain from LiDAR, over Småland, South Sweden
- 2015
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Ingår i: GFF. - : Informa UK Limited. - 2000-0863 .- 1103-5897. ; 137:4, s. 252-283
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Tidskriftsartikel (refereegranskat)abstract
- In this paper we synthesise previous sedimentological studies and new, high resolution, digital elevation models from southern Småland to investigate the evolution of a deglacial landscape. Starting approximately at the deglacial highest shoreline, there is a 20-40 km wide zone of ribbed and hummocky moraine that can be connected to the predominantly subaqueous Göteborg moraine. North of this there is a dramatic shift into streamlined terrain. Based on sedimentological investigations we conclude that these zones represent a sub/supraglacial stagnant-ice landsystem, followed by a subglacial active ice landsystem, which during their formations had very different thermal and process regimes along the ice-bed interface (freezing on/frozen versus wet-based basal regimes). New observations derived from high resolution, LiDAR digital elevation models reveals patterns of erosional/depositional and morphostratigraphic relationships that have not previously been observed, opening up gateways for future research.
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