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- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 6. |
- Kristan, Matej, et al.
(författare)
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The Ninth Visual Object Tracking VOT2021 Challenge Results
- 2021
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Ingår i: 2021 IEEE/CVF INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW 2021). - : IEEE COMPUTER SOC. - 9781665401913 ; , s. 2711-2738
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2021 is the ninth annual tracker benchmarking activity organized by the VOT initiative. Results of 71 trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in recent years. The VOT2021 challenge was composed of four sub-challenges focusing on different tracking domains: (i) VOT-ST2021 challenge focused on short-term tracking in RGB, (ii) VOT-RT2021 challenge focused on "real-time" short-term tracking in RGB, (iii) VOT-LT2021 focused on long-term tracking, namely coping with target disappearance and reappearance and (iv) VOT-RGBD2021 challenge focused on long-term tracking in RGB and depth imagery. The VOT-ST2021 dataset was refreshed, while VOT-RGBD2021 introduces a training dataset and sequestered dataset for winner identification. The source code for most of the trackers, the datasets, the evaluation kit and the results along with the source code for most trackers are publicly available at the challenge website(1).
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| 7. |
- Li, Fei, et al.
(författare)
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Integration of FeOOH and Zeolitic Imidazolate Framework-Derived Nanoporous Carbon as an Efficient Electrocatalyst for Water Oxidation
- 2018
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Ingår i: Advanced Energy Materials. - : Wiley-VCH Verlagsgesellschaft. - 1614-6832 .- 1614-6840. ; 8:10
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Tidskriftsartikel (refereegranskat)abstract
- As a cost-effective catalyst for the oxygen evolution reaction (OER), the potential use of FeOOH is hindered by its intrinsic poor electron conductivity. Here, the significant enhancement of OER activity and long-term stability of electrodeposited FeOOH on zeolitic imidazolate framework-derived N-doped porous carbons (NPCs) are reported. In alkaline media, FeOOH/NPC supported on nickel foam as a 3D electrode delivers a current density of 100 mA cm(-2) at a small overpotential of 230 mV and exhibits a low Tafel slope of 33.8 mV dec(-1) as well as excellent durability, making it one of the most active OER catalysts. Such high performance is attributed to a combined effect of the excellent electron conductivity of NPC and the synergy between FeOOH and NiO derived from Ni substrate.
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| 9. |
- Deng, Min, et al.
(författare)
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Genome-wide association analyses in Han Chinese identify two new susceptibility loci for amyotrophic lateral sclerosis
- 2013
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Ingår i: Nature Genetics. - : Nature Publishing Group. - 1061-4036 .- 1546-1718. ; 45:6, s. 697-
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Tidskriftsartikel (refereegranskat)abstract
- To identify susceptibility genes for amyotrophic lateral sclerosis (ALS), we conducted a genome-wide association study (GWAS) in 506 individuals with sporadic ALS and 1,859 controls of Han Chinese ancestry. Ninety top SNPs suggested by the current GWAS and 6 SNPs identified by previous GWAS were analyzed in an independent cohort of 706 individuals with ALS and 1,777 controls of Han Chinese ancestry. We discovered two new susceptibility loci for ALS at 1q32 (CAMK1G, rs6703183, P-combined = 2.92 x 10(-8), odds ratio (OR) = 1.31) and 22p11 (CABIN1 and SUSD2, rs8141797, P-combined = 2.35 x 10(-9), OR = 1.52). These two loci explain 12.48% of the overall variance in disease risk in the Han Chinese population. We found no association evidence for the previously reported loci in the Han Chinese population, suggesting genetic heterogeneity of disease susceptibility for ALS between ancestry groups. Our study identifies two new susceptibility loci and suggests new pathogenic mechanisms of ALS.
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| 10. |
- Yuan, Qin, et al.
(författare)
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Aridification signatures from fossil pollen indicate a drying climate in east-central Tibet during the late Eocene
- 2020
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Ingår i: Climate of the Past. - Vienna : European Geosciences Union (EGU). - 1744-9588 .- 1814-9332 .- 1814-9324. ; 16, s. 2255-2273
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Tidskriftsartikel (refereegranskat)abstract
- Central Asia experienced a number of significant elevational and climatic changes during the Cenozoic, but much remains to be understood regarding the timing and driving mechanisms of these changes as well as their influence on ancient ecosystems. Here, we describe the palaeoecology and palaeoclimate of a new section from the Nangqian Basin in Tibet, north-western China, dated as Bartonian (41.2–37.8 Ma; late Eocene) based on our palynological analyses. Located on the east-central part of what is today the Tibetan Plateau, this section is excellently placed for better understanding the palaeoecological history of Tibet following the Indo-Asian collision. Our new palynological record reveals that a strongly seasonal steppe–desert ecosystem characterized by drought-tolerant shrubs, diverse ferns, and an underlying component of broad-leaved forests existed in east-central Tibet during the Eocene, influenced by a southern monsoon. A transient warming event, possibly the middle Eocene climatic optimum (MECO; 40 Ma), is reflected in our record by a temporary increase in regional tropical taxa and a concurrent decrease in steppe–desert vegetation. In the late Eocene, a drying signature in the palynological record is linked to proto-Paratethys Sea retreat, which caused widespread long-term aridification across the region. To better distinguish between local climatic variation and farther-reaching drivers of Central Asian palaeoclimate and elevation, we correlated key palynological sections across the Tibetan Plateau by means of established radioisotopic ages and biostratigraphy. This new palynozonation illustrates both intra- and inter-basinal floral response to Qinghai–Tibetan uplift and global climate change during the Paleogene, and it provides a framework for the age assignment of future palynological studies in Central Asia. Our work highlights the ongoing challenge of integrating various deep time records for the purpose of reconstructing palaeoelevation, indicating that a multi-proxy approach is vital for unravelling the complex uplift history of Tibet and its resulting influence on Asian climate.
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