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- Rad, Negar Chalaki, et al.
(författare)
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Assessment of nanoliposomes loaded with daidzein for ameliorating diabetes in alloxan-induced mice: A promising nutraceutical approach
- 2023
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Ingår i: Journal of Functional Foods. - 1756-4646. ; 110
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Tidskriftsartikel (refereegranskat)abstract
- The present study synthesized and evaluated the effects of nanoliposomes loaded daidzein (NLD) in diabetic mice induced by alloxan. Five groups of Balb-c mice were treated for 5 weeks (Group 1 and 2 referred to as normal and diabetic control receiving normal feed). Group 3 and 4 were diabetic mice receiving 50 and 100 mg/kg/BW NLD respectively. Group 5, diabetic mice, received the standard drug, Metformin, 250 mg/kg/BW per day orally. Results indicated that NLD significantly (p < 0.05) improved the final body weight, triglyceride (TG), total cholesterol (TC) concentrations, low-density lipoprotein cholesterol and malondialdehyde (MDA) levels and enhanced the high-density lipoprotein. Furthermore, NLD enhanced the expression of CAT and suppressed iNOS gene in the pancreas. The mRNA expression of GLUT2 and GLUT4 also showed increase in the diabetic mice. Findings demonstrated the potential of NLD as a nutraceutical for the prevention and/or treatment of diabetes without toxic side effects.
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| 2. |
- Long, Maeve, et al.
(författare)
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DGAT1 activity synchronises with mitophagy to protect cells from metabolic rewiring by iron depletion
- 2022
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Ingår i: EMBO Journal. - : John Wiley & Sons. - 0261-4189 .- 1460-2075. ; 41
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Tidskriftsartikel (refereegranskat)abstract
- Mitophagy removes defective mitochondria via lysosomal elimination. Increased mitophagy coincides with metabolic reprogramming, yet it remains unknown whether mitophagy is a cause or consequence of such state changes. The signalling pathways that integrate with mitophagy to sustain cell and tissue integrity also remain poorly defined. We performed temporal metabolomics on mammalian cells treated with deferiprone, a therapeutic iron chelator that stimulates PINK1/PARKIN-independent mitophagy. Iron depletion profoundly rewired the metabolome, hallmarked by remodelling of lipid metabolism within minutes of treatment. DGAT1-dependent lipid droplet biosynthesis occurred several hours before mitochondrial clearance, with lipid droplets bordering mitochondria upon iron chelation. We demonstrate that DGAT1 inhibition restricts mitophagy in vitro, with impaired lysosomal homeostasis and cell viability. Importantly, genetic depletion of DGAT1 in vivo significantly impaired neuronal mitophagy and locomotor function in Drosophila. Our data define iron depletion as a potent signal that rapidly reshapes metabolism and establishes an unexpected synergy between lipid homeostasis and mitophagy that safeguards cell and tissue integrity.
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| 3. |
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| 4. |
- Youssef, Omar, et al.
(författare)
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Stool Microbiota Composition Differs in Patients with Stomach, Colon, and Rectal Neoplasms
- 2018
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Ingår i: Digestive Diseases and Sciences. - : SPRINGER. - 0163-2116 .- 1573-2568. ; 63:11, s. 2950-2958
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundMicrobial ecosystems that inhabit the human gut form central component of our physiology and metabolism, regulating and modulating both health and disease. Changes or disturbances in the composition and activity of this gut microbiota can result in altered immunity, inflammation, and even cancer.AimTo compare the composition and diversity of gut microbiota in stool samples from patient groups based on the site of neoplasm in the gastrointestinal tract (GIT) and to assess the possible contribution of the bacterial composition to tumorigenesis.MethodsWe studied gut microbiota by16S RNA gene sequencing from stool DNA of 83 patients, who were diagnosed with different GIT neoplasms, and 13 healthy individuals.ResultsAs compared to healthy individuals, stools of patients with stomach neoplasms had elevated levels of Enterobacteriaceae, and those with rectal neoplasms had lower levels of Bifidobacteriaceae. Lower abundance of Lactobacillaceae was seen in patients with colon neoplasms. Abundance of Lactobacillaceae was higher in stools of GIT patients sampled after cancer treatment compared to samples collected before start of any treatment. In addition to site-specific differences, higher abundances of Ruminococcus, Subdoligranulum and lower abundances of Lachnoclostridium and Oscillibacter were observed in overall GIT neoplasms as compared to healthy controlsConclusionOur study demonstrates that the alterations in gut microbiota vary according to the site of GIT neoplasm. The observed lower abundance of two common families, Lactobacillaceae and Bifidobacteriaceae, and the increased abundance of Enterobacteriaceae could provide indicators of compromised gut health and potentially facilitate GIT disease monitoring.
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