| 1. |
- Andersson, Hanna, et al.
(författare)
-
Natur på skolgården för lärande, hälsa och hållbarhet
- 2024. - 2024
-
Rapport (populärvet., debatt m.m.)abstract
- Gröna och artrika utemiljöer främjar barns och ungas välbefinnande och kunskap, bådegenom hälsofördelar kopplade till biologisk mångfald och genom att skapa förutsättningarför lek och lärande om natur och miljöfrågor. Skolgården skulle kunna bidra till allt detta,men är idag i hög grad en outnyttjad plats för biologisk mångfald och klimatanpassningav städer. I denna policy brief presenteras huvudsakliga motiv och möjliga åtgärder för attutveckla gröna miljöer och biologisk mångfald på skolgårdar och förskolegårdar.
|
|
| 2. |
- Uhlén, Mathias, et al.
(författare)
-
The human secretome
- 2019
-
Ingår i: Science Signaling. - : American Association for the Advancement of Science (AAAS). - 1945-0877 .- 1937-9145. ; 12:609
-
Tidskriftsartikel (refereegranskat)abstract
- The proteins secreted by human cells (collectively referred to as the secretome) are important not only for the basic understanding of human biology but also for the identification of potential targets for future diagnostics and therapies. Here, we present a comprehensive analysis of proteins predicted to be secreted in human cells, which provides information about their final localization in the human body, including the proteins actively secreted to peripheral blood. The analysis suggests that a large number of the proteins of the secretome are not secreted out of the cell, but instead are retained intracellularly, whereas another large group of proteins were identified that are predicted to be retained locally at the tissue of expression and not secreted into the blood. Proteins detected in the human blood by mass spectrometry-based proteomics and antibody-based immuno-assays are also presented with estimates of their concentrations in the blood. The results are presented in an updated version 19 of the Human Protein Atlas in which each gene encoding a secretome protein is annotated to provide an open-access knowledge resource of the human secretome, including body-wide expression data, spatial localization data down to the single-cell and subcellular levels, and data about the presence of proteins that are detectable in the blood.
|
|
| 3. |
|
|
| 4. |
- Eriksson, Ulrika, 1972-, et al.
(författare)
-
Comparison of PFASs contamination in the freshwater and terrestrial environments by analysis of eggs from osprey (Pandion haliaetus), tawny owl (Strix aluco), and common kestrel (Falco tinnunculus)
- 2016
-
Ingår i: Environmental Research. - San Diego, USA : Academic Press. - 0013-9351 .- 1096-0953. ; 149, s. 40-47
-
Tidskriftsartikel (refereegranskat)abstract
- The level of PFAS (per- and polyfluorinated alkyl substances) contamination in freshwater and terrestrial Swedish environments in 2013/2014 was assessed by analyzing a range of perfluorinated alkyl acids, fluorotelomer acids, sulfonamides, sulfonamidoethanols and polyfluoralkyl phosphate diesters (diPAPs) in predator bird eggs. Stable isotopes ((13)C and (15)N) were analyzed to elucidate the dietary source. The tawny owl (Strix aluco, n=10) and common kestrel (Falco tinnunculus, n=40), two terrestrial species, and the osprey (Pandion haliaetus, n=30), a freshwater specie were included. In addition, a temporal trend (1997-2001, 2008-2009, 2013) in osprey was studied as well. The PFAS profile was dominated by perfluorooctane sulfonic acid (PFOS) in eggs from osprey and tawny owl, while for common kestrel perfluorinated carboxylic acids (∑PFCA) exceeded the level of PFOS. PFOS concentration in osprey eggs remained at the same level between 1997 and 2001 and 2013. For the long-chained PFCAs, there were a significant increase in concentrations in osprey eggs between 1997 and 2001 and 2008-2009. The levels of PFOS and PFCAs were about 10 and five times higher, respectively, in osprey compared to tawny owl and common kestrel. Evidence of direct exposure from PFCA precursor compounds to birds in both freshwater and terrestrial environment was observed. Low levels of diPAPs were detected in a few samples of osprey (<0.02-2.4ng/g) and common kestrel (<0.02-0.16ng/g) eggs, and 6:2 FTSA was detected in a majority of the osprey eggs (<6.3-52ng/g). One saturated telomer acid (7:3 FTCA), which is a transformation marker from precursor exposure, was detected in all species (<0.24-2.7ng/g). The (15)N data showed higher levels in osprey eggs compared to tawny owl and common kestrel, indicating that they feed on a 2-3 times higher trophic level. We conclude that ospreys are continuously exposed to PFAS at levels where adverse toxic effects have been observed in birds.
|
|
| 5. |
- Liu, Yongsheng, et al.
(författare)
-
Biologic Evaluation of a Heterodimeric HER2-Albumin Targeted Affibody Molecule Produced by Chemo-Enzymatic Peptide Synthesis
- 2022
-
Ingår i: Pharmaceutics. - : MDPI. - 1999-4923. ; 14:11
-
Tidskriftsartikel (refereegranskat)abstract
- Targeted molecular radiation therapy is a promising emerging treatment modality in oncology, and peptide synthesis may shorten the time to reach the clinical stage. In this study, we have explored Chemo-Enzymatic Peptide Synthesis, or CEPS, as a new means of producing a therapeutic HER2 targeted Affibody((R)) molecule, comprising a C-terminal albumin binding domain (ABD) for half-life extension and a total length of 108 amino acids. In addition, a DOTA moiety could be incorporated at N-terminus directly during the synthesis step and subsequently utilized for site-specific radiolabeling with the therapeutic radionuclide Lu-177. Retained thermodynamic stability as well as retained binding to both HER2 and albumin was verified. Furthermore, HER2 binding specificity of the radiolabeled Affibody molecule was confirmed by an in vitro saturation assay showing a significantly higher cell-bound activity of SKOV-3 (high HER2 expression) compared with BxPC3 (low HER2 expression), both in the presence and absence of HSA. In vivo evaluation in mice bearing HER2 expressing xenografts also showed specific tumor targeting as well as extended time in circulation and reduced kidney uptake compared with a HER2 targeted Affibody molecule without the ABD moiety. To conclude, we have demonstrated that CEPS can be used for production of Affibody-fusion molecules with retained in vitro and in vivo functionality.
|
|
| 6. |
- Tegel, Hanna, et al.
(författare)
-
High throughput generation of a resource of the human secretome in mammalian cells
- 2020
-
Ingår i: New Biotechnology. - : Elsevier BV. - 1871-6784 .- 1876-4347. ; 58, s. 45-54
-
Tidskriftsartikel (refereegranskat)abstract
- The proteins secreted by human tissues and blood cells, the secretome, are important both for the basic understanding of human biology and for identification of potential targets for future diagnosis and therapy. Here, a high-throughput mammalian cell factory is presented that was established to create a resource of recombinant full-length proteins covering the majority of those annotated as 'secreted' in humans. The full-length DNA sequences of each of the predicted secreted proteins were generated by gene synthesis, the constructs were transfected into Chinese hamster ovary (CHO) cells and the recombinant proteins were produced, purified and analyzed. Almost 1,300 proteins were successfully generated and proteins predicted to be secreted into the blood were produced with a success rate of 65%, while the success rates for the other categories of secreted proteins were somewhat lower giving an overall one-pass success rate of ca. 58%. The proteins were used to generate targeted proteomics assays and several of the proteins were shown to be active in a phenotypic assay involving pancreatic beta-cell dedifferentiation. Many of the proteins that failed during production in CHO cells could be rescued in human embryonic kidney (HEK 293) cells suggesting that a cell factory of human origin can be an attractive alternative for production in mammalian cells. In conclusion, a high-throughput protein production and purification system has been successfully established to create a unique resource of the human secretome.
|
|
| 7. |
- Andersson, C David, et al.
(författare)
-
Discovery of Ligands for ADP-Ribosyltransferases via Docking-Based Virtual Screening
- 2012
-
Ingår i: Journal of Medicinal Chemistry. - : American Chemical Society (ACS). - 0022-2623 .- 1520-4804. ; 55:17, s. 7706-7718
-
Tidskriftsartikel (refereegranskat)abstract
- The diphtheria toxin-like ADP-ribosyltransferases (ARTDs) are an enzyme family that catalyses the transfer of ADP-ribose units onto substrate proteins, using nicotinamide adenine dinucleotide (NAD(+)) as a co-substrate. They have a documented role in chromatin remodelling and DNA repair; and inhibitors of ARTD1 and 2 (PARP1 and 2) are currently in clinical trials for the treatment of cancer. The detailed function of most other ARTDs is still unknown. Using virtual screening we identified small ligands of ARTD7 (PARP15/BAL3) and ARTD8 (PARP14/BAL2). Thermal-shift assays confirmed that 16 compounds, belonging to eight structural classes, bound to ARTD7/ARTD8. Affinity measurements with isothermal titration calorimetry for two isomers of the most promising hit compound confirmed binding in the low micromolar range to ARTD8. Crystal structures showed anchoring of the hits in the nicotinamide pocket. These results form a starting point in the development of chemical tools for the study of the role and function of ARTD7 and ARTD8.
|
|
| 8. |
- Bahr, Adam, et al.
(författare)
-
Growth of ectomycorrhizal fungal mycelium along a Norway spruce forest nitrogen deposition gradient and its effect on nitrogen leakage
- 2013
-
Ingår i: Soil Biology and Biochemistry. - : Elsevier BV. - 0038-0717 .- 1879-3428. ; 59, s. 38-48
-
Tidskriftsartikel (refereegranskat)abstract
- Almost all boreal and temperate forest tree species live in symbiosis with ectomycorrhizal fungi (EMF); the trees transfer carbon (C) to the fungi in exchange for nutrients and water. Several studies have shown that experimental application of inorganic nitrogen (N) represses production of EMF extramatrical mycelia (EMM), but studies along N deposition gradients are underrepresented. Other environmental variables than N may influence EMM production and in this study we included 29 thoroughly monitored Norway spruce stands from a large geographical region in Sweden in order to evaluate the importance of N deposition on EMM growth and N leaching in a broader context. It was concluded that N deposition was the most important factor controlling EMM production and that the amounts typically deposited in boreal and boreo-nemoral regions can be sufficient to reduce EMM growth. Other factors, such as phosphorus status and pH, were also correlated with EMM production and should be considered when predicting EMM growth and N leaching. We also showed that EMM production substantially contributed to the C sequestration (320 kg ha(-1) yr(-1)), suggesting that it should be included in C cycle modelling. Furthermore, EMF are probably important for the N retention capacity since high N leaching coincided with low EMM growth. However, it was not possible to differentiate between the effects of EMF and the direct effect of N deposition on N leaching in the present study.
|
|
| 9. |
- Bertmar, Sofia, et al.
(författare)
-
Who's Most Targeted and Does My New Adblocker Really Help : A Profile-based Evaluation of Personalized Advertising
- 2021
-
Ingår i: Proc. ACM CCS Workshop on Privacy in the Electronic Society (ACM WPES @CCS). - New York, NY, USA : ACM Digital Library. - 9781450385275
-
Konferensbidrag (refereegranskat)abstract
- There is limited prior work studying how the ad personalization experienced by different users is impacted by the use of adblockers, geographic location, the user's persona, or what browser they use. To address this void, this paper presents a novel profile-based evaluation of the personalization experienced by carefully crafted user profiles. Our evaluation framework impersonates different users and captures how the personalization changes over time, how it changes when adding or removing an extension, and perhaps most importantly how the results differ depending on the profile's persona (e.g., interest, occupation, age, gender), geographic location (US East, US West, UK), what browser extension they use (none, AdBlock, AdBlock Plus, Ghostery, CatBlock), what browser they use (Chrome, Firefox), and whether they are logged in to their Google account. By comparing and contrasting observed differences we provide insights that help explain why some user groups may feel more targeted than others and why some people may feel even more targeted after having turned on their adblocker.
|
|
| 10. |
- Condelius, Anna, et al.
(författare)
-
Förstärkt hemtjänst - en utvärdering av projektet med fokus på brukare och personal
- 2013
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- I Malmö stad, stadsområdesförvaltning norr, före detta stadsdelsförvaltning Centrum, pågår sedan 2011 projektet förstärkt hemtjänst. Förstärkt hemtjänst är ett alternativ till korttidsboende, vilket innebär att brukaren under en kortare tidsperiod får extra stöd och hjälp i hemmet. Det övergripande syftet med detta dokument har varit att utvärdera projektet förstärkt hemtjänst i stadsdel Centrum. Utvärderingen ska utgöra en grund till ett beslut om projektet ska bli en permanent del av verksamheten. Utvärderingen har haft särskilt fokus på brukarens tillfredsställelse med erhållen vård och omsorg, samt omsorgspersonalens arbetssituation och arbetstillfredsställelse. Brukare och personal i den ordinarie hemtjänsten i samma stadsdel har utgjort jämförelsegrupp. Data har inhämtats genom enkäter till brukare och personal i både den förstärkta hemtjänsten och i den ordinarie hemtjänsten.
|
|
