| 1. |
- Olsson, Peter, et al.
(författare)
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Minnesteckningar över avlidna ledamöter 2010. Särtryck ur KVVS Årsbok 2011
- 2011
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Ingår i: Kungl. Vetenskaps- och Vitterhets-Samhället i Göteborg (KVVS).
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Minnesteckningar. Kungl. Vetenskaps- och Vitterhets-Samhället - ledamöter avlidna 2010. Bengt Holmberg, Gunilla Åkerström-Hougen, Gunnar Harling, Jan S. Nilsson, Ulf Lagerkvist, Erik Frykman, Sigvard Rubenowitz
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| 2. |
- Almståhl, Annica, 1973, et al.
(författare)
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Fermentation of sugars and sugar alcohols by plaque Lactobacillus strains
- 2013
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Ingår i: Clinical Oral Investigations. - : Springer Science and Business Media LLC. - 1432-6981 .- 1436-3771. ; 17:6, s. 1465-1470
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Tidskriftsartikel (refereegranskat)abstract
- Objective The objective was to analyse the ability of Lactobacillus strains isolated from supragingival plaque of subjects with hyposalivation and from healthy controls to ferment sugars and sugar alcohols. Material and methods Fifty strains isolated from interproximal plaque from subjects with radiation-induced hyposalivation (25 strains), subjects with primary Sjögren’s syndrome (16 strains) and from subjects with normal salivary secretion rate (9 strains) were tested. Growth and pH were determined after 24 and 48 h of anaerobic incubation in vials containing basal media with 1 % of glucose, fructose, sucrose, mannitol, sorbitol or xylitol. Results No differences between strains isolated from hyposalivated subjects and controls were detected. All strains lowered the pH to <5.0 from fructose and the majority of the strains from glucose and sucrose. A pH of <5.5 was seen for 52 % of the strains using mannitol, 50 % using sorbitol and 36 % using xylitol. The ability to produce acids from sugars and sugar alcohols was highest among strains of Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus paracasei and lowest among Lactobacillus fermentum strains. Conclusion A large number of Lactobacillus strains are able to ferment not only sugars but also the sugar substitutes mannitol, sorbitol and xylitol to pH levels critical for enamel demineralisation. Clinical relevance Our findings suggest that products containing mannitol, sorbitol and/or xylitol may contribute to the acidogenic potential of the dental plaque and especially in hyposalivated subjects with high numbers of lactobacilli.
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| 3. |
- Almståhl, Annica, 1973, et al.
(författare)
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Lactobacillus species in supragingival plaque in subjects with hyposalivation
- 2004
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Ingår i: International journal of dental hygiene. - : Wiley. - 1601-5029 .- 1601-5037. ; 2:3
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Konferensbidrag (refereegranskat)abstract
- The aim was to analyse frequency and proportion of Lactobacillus species in supragingival plaque in subjects with hyposalivation and the Lactobacillus species ability to ferment sucrose, mannitol, sorbitol and xylitol.Material and methods: Ten subjects treated with radiation therapy (RT), 10 subjects with primary Sjogren's syndrome (pSS), and matched controls were included. Supragingival plaque was collected interproximally 12-13 and 15-16 and analysed using cultivation technique. Lactobacillus colonies were randomly selected and stored at -70 degrees C. The Lactobacillus strains fermentation pattern was analysed using basal medium with 1% of respective carbohydrate/sugar alcohol added.Results: Lactobacilli were more frequent at site 15-16 than at site 12-13. Lactobacilli were about twice as frequent in the pSS and RT groups than in their respective control group. For those harbouring lactobacilli, the proportion of the total count at site 15-16 was 13 +/- 27% for the RT group and 0.003 +/- 0.003% for their controls, 3.2 +/- 5.8% for the pSS group and 0.06 +/- 0.2% for their controls. We have further analysed 28 of 114 collected Lactobacillus strains. All strains gave a pH <6.0 and at 95% a pH <5.5 at sucrose fermentation. Mannitol could be fermented by 82% of the strains, sorbitol by 75% and xylitol by 64%. A pH <5.5 was obtained for 82% of the strains with mannitol, 75% with sorbitol and 32% with xylitol.Conclusion: Subjects with hyposalivation have a marked increase in lactobacilli. Of the strains analysed this far 95% gave a pH <5.5 at sucrose-fermentation. A pH <5.5 was obtained for 82% of the strains with mannitol, 75% with sorbitol and 32% with xylitol. As those sugar substitutes are included in tooth pastes, chewing gums and saliva-stimulating tablets and sprays, it is likely that the lactobacilli are further promoted in subjects with hyposalivation.
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| 4. |
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| 5. |
- Carlén, Anette, 1950, et al.
(författare)
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Human minor and major gland saliva proteins and ability to mediate Actinomyces naeslundii adherence.
- 2004
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969. ; 49:3, s. 177-81
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Tidskriftsartikel (refereegranskat)abstract
- Bacteria-binding components and the ability to mediate bacterial adhesion to the tooth surface have been thoroughly studied in major salivary gland secretions. Our knowledge on the bacteria binding activity in minor gland saliva is, however, limited. In this study, proteins were examined in parallel in minor (palatal, buccal and labial) and major (parotid and submandibular/sublingual) salivary gland secretions in one subject using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The adherence of early colonizing Actinomyces naeslundii to pellicles formed from the secretions on hydroxyapatite beads was also examined. Amylase, IgA, proline-rich proteins and the high-molecular-weight glycoproteins, agglutinins, were detected in all saliva tested. Carbohydrate-reactive antibodies recognized the low-molecular weight mucin, MUC 7 in submandibular/sublingual saliva only. A. naeslundii strain 12104 adhered to all pellicles and especially to the buccal gland saliva pellicles. Strain LY7 adhered in highest numbers to the submandibular/sublingual saliva pellicles. It also bound in considerable numbers to parotid and palatal saliva pellicles but not to the ones formed from buccal and labial gland saliva. Our findings indicate that several bacteria-binding components are secreted in both minor and major gland saliva. The adherence-promoting ability of the various gland secretions differs, however.
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| 6. |
- Eliasson, Lars, 1940, et al.
(författare)
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An update on minor salivary gland secretions
- 2010
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Ingår i: European journal of oral sciences. - : Wiley. - 1600-0722 .- 0909-8836. ; 118:5, s. 435-42
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Tidskriftsartikel (refereegranskat)abstract
- In this article, the literature on minor salivary gland secretion rates, composition, and function is reviewed. Measurements of the minor salivary gland secretion rates and composition are complicated, and the secretions display large biological variability. Despite this, some characteristics of these secretions have been found repeatedly in independent investigations. Minor gland saliva varies between different oral sites. Buccal saliva flow is higher than labial saliva flow, which in turn is usually higher than the palatal gland secretion rate. It is generally agreed that minor gland saliva is important for the whole saliva composition, and especially for the secretory immunoglobulin A and mucins. The secretion from these glands seems also important for subjective feelings of dry mouth and general wellbeing. Further research is essential for understanding the role of these secretions for oral, as well as for general, health.
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| 7. |
- Eliasson, Lars, 1940, et al.
(författare)
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Dental plaque pH and micro-organisms during hyposalivation.
- 2006
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Ingår i: Journal of dental research. - 0022-0345. ; 85:4, s. 334-8
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Tidskriftsartikel (refereegranskat)abstract
- We have previously reported that minor gland and whole saliva flow rates and salivary proteins showed differences in individuals with primary Sj?gren's syndrome or head and neck radiation therapy, compared with controls (Eliasson et al., 2005). We now hypothesize that pH and number of acidogenic micro-organisms in dental plaque as well as saliva buffering capacity also differ in these individuals. Plaque pH was measured by the microtouch method up to 60 min after a sucrose rinse. Plaque collected from the same sites was analyzed for counts of total and acidic micro-organisms. Compared with their controls, the irradiated group but not the Sj?gren's syndrome group displayed significantly lower plaque pH, increased numbers of lactobacilli and Candida species, as well as reduced buffering capacity. Stepwise regression tests suggested that the buccal minor-salivary-gland secretion rate in the test groups and counts of mutans streptococci in the controls were of significant importance for dental plaque pH.
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| 8. |
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| 9. |
- Eliasson, Lars, 1940, et al.
(författare)
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Feeling of dry mouth in relation to whole and minor gland saliva secretion rate.
- 2009
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Ingår i: Archives of oral biology. - : Elsevier BV. - 1879-1506 .- 0003-9969. ; 54:3, s. 263-7
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Tidskriftsartikel (refereegranskat)abstract
- Previous reports indicate an association between whole saliva secretion rate and xerostomia, but the association is not always found in individuals complaining of dry mouth. In the present study, we hypothesised that the minor salivary gland secretions could affect subjective feelings of oral dryness in individuals with normal or low whole saliva secretion rate. 142 individuals, aged 18-82 years, participated in the study. Feelings of oral dryness were assessed separately for day- and nighttime by visual analogue scales. Resting and stimulated whole saliva flow rates were measured by conventional methods, and minor palatal, buccal and labial saliva secretion rates by the Periotron method. Twenty-three percent of the women and 15% of the men reported feelings of dry mouth during day and night. The results showed statistically significant lower resting and stimulated whole and minor labial gland saliva secretion rates in subjects reporting oral dryness during both day and night compared with non-complainers. In groups of individuals with normal simulated whole saliva secretion, those who complained from dry mouth both day and night had statistically significant lower labial secretion rate compared with those who had no complaints. Although not statistically significant, this difference was seen also in the groups with fewer individuals who had low whole saliva flow. In summary, our findings suggested that the labial gland saliva might affect subjective feelings of dry mouth both in individuals with normal and subnormal whole saliva flow.
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| 10. |
- Eliasson, Lars, 1940, et al.
(författare)
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Minor gland saliva flow rate and proteins in subjects with hyposalivation due to Sjogren's syndrome and radiation therapy.
- 2005
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969. ; 50:3, s. 293-9
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Tidskriftsartikel (refereegranskat)abstract
- In this study, the secretion rate and IgA, albumin and lactoferrin concentrations in minor labial and buccal gland saliva were investigated in individuals with hyposalivation due to primary Sjogren's syndrome (pSS; 10 subjects) or head and neck radiation therapy (RT; 10 subjects) and in their matched controls. Whole saliva was similarly examined. The minor gland saliva flow was measured using the Periotron method. IgA, albumin and lactoferrin concentrations were analysed by ELISA techniques. A general finding was that the flow rate and protein concentrations were lower in labial than in buccal gland saliva. In both hyposalivation groups, the labial minor gland saliva secretion rate was lowered compared to their respective controls. The buccal gland saliva flow rate was significantly reduced in the RT group only. IgA and albumin concentrations were not different from the controls in the labial secretions. The concentration of lactoferrin was increased in the RT group. In buccal saliva, the concentrations of all proteins examined but pSS IgA, were increased compared to the controls. Reduced flow rate and increased protein concentrations were seen for whole saliva where the lactoferrin concentration was higher in RT than in pSS subjects. Thus, our findings suggested that minor gland saliva flow rate and protein concentrations are affected in RT and pSS subjects and to highest extent in the former.
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