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- Persson, Bengt L., et al.
(författare)
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Energy-linked nicotinamide nucleotide transhydrogenase : Properties of proton-translocating mitochondrial transhydrogenase from beef heart purified by fast protein liquid chromatography
- 1984
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Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 259, s. 8626-8632
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Tidskriftsartikel (refereegranskat)abstract
- Mitochondrial nicotinamide nucleotide transhydrogenasefrom beef heart was purified by a novel procedureinvolving fast protein liquid chromatography andcharacterized with respect to molecular and catalyticproperties. The method is reproducible, gives highlypure transhydrogenase as judged by silver staining,and can be modified to produce large amounts of puretranshydrogenase protein suitable for e.g. sequencingand other protein chemical studies.Transhydrogenase purified by fast protein liquidchromatography is reconstitutively active and pumpsprotons as indicated by an extensive quenching of 9-aminoacridine fluorescence. Under conditions whichgenerate a proton gradient in the absence of a membranepotential the activity of reconstituted transhydrogenaseis close to zero indicating a complete andproper incorporation in the membrane and a preferentialregulation of the enzyme by a proton gradientrather than a membrane potential.Treatment of reconstituted transhydrogenase withN,N-dicyclohexylcarbodiimide results in an inhibitionof proton pump activity without an effect on uncoupledcatalytic activity, suggestingt hat proton translocationand catalytic activities are not obligatory linked orthat this agent separates proton pumping from thecatalytic activity.
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- Eklund, Arne, 1957-, et al.
(författare)
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Short-term results of a randomized clinical trial comparing Lichtenstein open repair with totally extraperitoneal laparoscopic inguinal hernia repair
- 2006
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Ingår i: British Journal of Surgery. - : Oxford University Press (OUP). - 0007-1323 .- 1365-2168. ; 93:9, s. 1060-1068
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Tidskriftsartikel (refereegranskat)abstract
- Background: Laparoscopic herniorrhaphy has emerged as a recognized operative method for inguinal hernia repair. This study compared the short-term results of two tension-free methods of repair: totally extraperitoneal (TEP) laparoscopic patch repair and the open Lichtenstein mesh technique. Methods: A total of 1513 men from 11 hospitals who presented with a primary unilateral inguinal hernia were randomized to one of the two methods. Operating time, short-term complications, reoperations, postoperative pain, consumption of analgesics, sick leave and time to resumption of normal physical activities were recorded. Results: Some 1371 of the 1513 men underwent surgery, 665 in the TEP group and 706 in the Lichtenstein group. The median duration of operation was 55 min for both procedures and 91.0 per cent of die patients in both groups were discharged on the day of operation. Patients in the TEP group experienced less postoperative pain (P < 0.001), consumed fewer analgesics (P < 0.001), had a shorter period of sick leave (7 versus 12 days; P < 0.001) and a shorter time to resumption of normal physical activity (20 versus 31 days; P < 0.001). Conclusion: The TEP technique took no longer to perform, and was associated with less postoperative pain, a shorter period of sick leave and a faster recovery, compared with open Lichtenstein hernia repair.
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