| 1. |
- Sundin, Maria, 1965, et al.
(författare)
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Mars - a target for teachers and science students
- 2020
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Ingår i: Contribution to IAU 367S, Education and Heritage in the Era of Big Data in Astronomy, 8-12 December 2020..
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- A case study is here presented of an interdisciplinary course about Mars for teachers and science students. We aim to share the experience of creating an interdisciplinary approach with lecturers spanning physics, geology, radiation physics and philosophy. Issues in ethics, morality, rights and obligations, conflict management and human psychology as well as rocket orbits, fuel economy, radiation hazards and knowledge of the solar system have proven to be a valued and successful initiative for the further training of teachers and science students. The focus of the course is on planning for a journey with humans to the planet Mars. This provides a great opportunity to package complex societal problems in a physics context. The course is offered with a special sustainability content mark. Mankind has always had a strong and dependent relationship with the physical landscape. The land has given us food and shelter but also imposed challenges and disasters. Understanding the physical environment has been crucial for our survival and development. The same will be equally, or more important for Mars where life conditions are much more extreme. We highlight similarities and differences in the geologic processes that have shaped Earth and Mars. What conditions do the future explorers on Mars have to manage? We then enter the modern era and explore the dynamic Martian landscape of today. Also, by learning to read the landscape we may find locations of shelter such as vast systems of lava tubes, or locations of essential resources such as preserved glacial ice etc. A journey to Mars will cause substantially higher personal irradiation than obtained on Earth. The radiation part of the course lectures starts with defining the different radiation types and the biological effects these different types of radiation will cause. Then, the difference between the irradiation on Earth to the elevated irradiation in space and on Mars is described. Thereafter, it is discussed if this elevated radiation burden can cause acute biological effects, e.g. fatigue, vomiting and death, and late biological effects as cancer induction. Last, possible radiation protection strategies are described and discussed. The philosophy of space exploration consists of philosophical approaches to ethics, presently applied to the topic of Mars exploration and colonization, with environmental ethics (anthropocentric vs ecocentric) and value theory at its core. Four main uses of philosophy are distinguished: ethics, aesthetics, cognition and existentialism. Research has shown that visual representation is an important part for students to be able to create a deeper understanding of concepts as well as context about the material that is taught. Interdisciplinary and complex societal problems have also been shown to be important in science teaching. One way for the teacher to develop his/her teaching is to take further education courses in universities whose focus is to seek and discuss the complex societal problems as well as its solutions from a physics and teacher perspective. Future research could be done on the impact of this course on the education in different levels.
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| 2. |
- Sundin, Maria, 1965, et al.
(författare)
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Mars – a target for teachers and science students
- 2021
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Ingår i: Proceedings of the International Astronomical Union, Vol 15, Symposium 367S. - 1743-9213 .- 1743-9221.
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Konferensbidrag (refereegranskat)abstract
- An interdisciplinary course about Mars for teachers and science students is presented. The focus of the course is on planning for a journey with humans to the planet Mars. Issues in ethics, morality, rights and obligations, conflict management and human psychology as well as rocket orbits, fuel economy, radiation hazards and knowledge of the solar system are included. Examination of the teacher students include interpretation of the course material for future pedagogical usage.
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| 3. |
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| 4. |
- Enger, Jonas, 1966, et al.
(författare)
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Optical tweezers applied to a microfluidic system
- 2004
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Ingår i: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 4, s. 196-200
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Tidskriftsartikel (refereegranskat)abstract
- We will demonstrate how optical tweezers can be combined with a microfluidic system to create a versatile microlaboratory. Cells are moved between reservoirs filled with different media by means of optical tweezers. We show that the cells, on a timescale of a few seconds, can be moved from one reservoir to another without the media being dragged along with them. The system is demonstrated with an experiment where we expose E. coli bacteria to different fluorescent markers. We will also discuss how the system can be used as an advanced cell sorter. It can favorably be used to sort out a small fraction of cells from a large population, in particular when advanced microscopic techniques are required to distinguish various cells. Patterns of channels and reservoirs were generated in a computer and transferred to a mask using either a sophisticated electron beam technique or a standard laser printer. Lithographic methods were applied to create microchannels in rubber silicon (PDMS). Media were transported in the channels using electroosmotic flow. The optical system consisted of a combined confocal and epi-fluorescence microscope, dual optical tweezers and a laser scalpel.
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| 5. |
- Eriksson, Emma, 1980, et al.
(författare)
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A microfluidic system in combination with optical tweezers for analyzing rapid and reversible cytological alterations in single cells upon environmental changes
- 2007
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Ingår i: Lab on a chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 7:1, s. 71-76
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Tidskriftsartikel (refereegranskat)abstract
- We report on the development of an experimental platform where epi-fluorescence microscopy and optical tweezers are combined with a microfluidic system to enable the analysis of rapid cytological responses in single cells. The microfluidic system allows two different media to be merged in a Y-shaped channel. Microscale channel dimensions ensure purely laminar flow and, as a result, an environmental gradient can be created between the two media. Optical tweezers are used to move a single trapped cell repeatedly between the different environments. The cell is monitored continuously by fluorescence microscopy during the experiment. In a first experiment on yeast (Saccharomyces cerevisiae) we observed changes in cell volume as the cell was moved between environments with different osmolarity. This demonstrated that the platform allowed analysis of cytological alterations on a time scale shorter than 0.2 s. In a second experiment we observed the spatial migration of the Yap1p transcription factor fused to GFP as a cell was moved from an environment of low to high oxidative capacity. The system is universal allowing the response to numerous environmental changes to be studied on the sub second time scale in a variety of model cells. We intend to use the platform to study how the age of cells, their progression through the cell cycle, or their genetic landscape, alter their capacity (kinetics and amplitude) to respond to environmental changes.
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| 6. |
- Eriksson, Emma, 1980, et al.
(författare)
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Holographic optical tweezers combined with a microfluidic device for exposing cells to fast environmental changes
- 2007
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Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 0277-786X.
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Konferensbidrag (refereegranskat)abstract
- Optical manipulation techniques have become an important research tool for single cell experiments in microbiology. Using optical tweezers, single cells can be trapped and held during long experiments without risk of cross contamination or compromising viability. However, it is often desirable to not only control the position of a cell, but also to control its environment. We have developed a method that combines optical tweezers with a microfluidic device. The microfluidic system is fabricated by soft lithography in which a constant flow is established by a syringe pump. In the microfluidic system multiple laminar flows of different media are combined into a single channel, where the fluid streams couple viscously. Adjacent media will mix only by diffusion, and consequently two different environments will be separated by a mixing region a few tens of micrometers wide. Thus, by moving optically trapped cells from one medium to another we are able to change the local environment of the cells in a fraction of a second. The time needed to establish a change in environment depends on several factors such as the strength of the optical traps and the steepness of the concentration gradient in the mixing region. By introducing dynamic holographic optical tweezers several cells can be trapped and analyzed simultaneously, thus shortening data acquisition time. The power of this system is demonstrated on yeast (Saccharomyces cerevisiae) subjected to osmotic stress, where the volume of the yeast cell and the spatial localization of green fluorescent proteins (GFP) are monitored using fluorescence microscopy.
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| 7. |
- Eriksson, Emma, 1980, et al.
(författare)
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Optical manipulation and microfluidics for studies of single cell dynamics
- 2007
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Ingår i: Journal of Optics. A, Pure and applied optics. - 1464-4258 .- 1741-3567 .- 1361-6617. ; 9:8, s. 113-121
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Tidskriftsartikel (refereegranskat)abstract
- Most research on optical manipulation aims towards investigation and development of the system itself. In this paper we show how optical manipulation, imaging and microfluidics can be combined for investigations of single cells. Microfluidic systems have been fabricated and are used, in combination with optical tweezers, to enable environmental changes for single cells. The environment within the microfluidic system has been modelled to ensure control of the process. Three biological model systems have been studied with different combinations of optical manipulation, imaging techniques and microfluidics. In Saccharomyces cerevisiae, environmentally induced size modulations and spatial localization of proteins have been studied to elucidate various signalling pathways. In a similar manner the oxygenation cycle of single red blood cells was triggered and mapped using Raman spectroscopy. In the third experiment the forces between the endoplasmic reticulum and chloroplasts were studied in Pisum sativum and Arabidopsis thaliana. By combining different techniques we make advanced biological research possible, revealing information on a cellular level that is impossible to obtain with traditional techniques.
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| 8. |
- Galan, D., et al.
(författare)
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A remote laboratory for optical levitation of charged droplets
- 2018
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Ingår i: European Journal of Physics. - : IOP Publishing. - 0143-0807 .- 1361-6404. ; 39:4
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Tidskriftsartikel (refereegranskat)abstract
- We present a remotely controlled experiment in which liquid droplets are levitated by a vertically aligned focused laser beam. The droplets levitate at the point where the photon pressure of the focused laser beam balances the gravitational force. The size of a trapped droplet can be measured by detecting the diffraction pattern created by the trapping laser light. The charge on the trapped droplet can thereafter be determined by observing its motion when a vertically directed electrical field is applied. This experiment allows a student to study many fundamental physics processes, such as photon pressure, diffraction of light, or the motion of charged particles in electrical fields. The complexity of the experiments and the concept studied make this suitable for advanced studies in physics. The laser power required in the experiment is about 1 W, which is a thousand times greater than the value of 1 mW at which lasers begin to be capable of causing harm to eyes; high voltages are also used. Further, the cost of the equipment is relatively high, which limits its availability to most undergraduate teaching laboratories. It thus constitutes an ideal experiment for remote control.
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| 9. |
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| 10. |
- Goksör, Mattias, 1975, et al.
(författare)
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Optical manipulation in combination with multiphoton microscopy for single-cell studies
- 2004
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Ingår i: Applied Optics. ; 43:25, s. 4831-4837
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Tidskriftsartikel (refereegranskat)abstract
- We demonstrate how optical tweezers can be incorporated into a multiphoton microscope to achieve three-dimensional imaging of trapped cells. The optical tweezers, formed by a cw 1064 nm Nd:YVO4 laser, were used to trap live yeast cells in suspension while the 4′, 6-diamidino-2-phenylindole-stained nucleus was imaged in three dimensions by use of a pulsed femtosecond laser. The trapped cell was moved in the axial direction by changing the position of an external lens, which was used to control the divergence of the trapping laser beam. This gives us a simple method to use optical tweezers in the laser scanning of confocal and multiphoton microscopes. It is further shown that the same femtosecond laser as used for the multiphoton imaging could also be used as laser scissors, allowing us to drill holes in the membrane of trapped spermatozoa.
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