| 1. |
- Karim, Sazzad, et al.
(författare)
-
Improved drought tolerance without undesired side effects in transgenic plants producing trehalose
- 2007
-
Ingår i: Plant Molecular Biology. - : Springer Science and Business Media LLC. - 0167-4412 .- 1573-5028. ; 64:4, s. 371-386
-
Tidskriftsartikel (refereegranskat)abstract
- Most organisms naturally accumulating trehalose upon stress produce the sugar in a two-step process by the action of the enzymes trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP). Transgenic plants overexpressing TPS have shown enhanced drought tolerance in spite of minute accumulation of trehalose, amounts believed to be too small to provide a protective function. However, overproduction of TPS in plants has also been found combined with pleiotropic growth aberrations. This paper describes three successful strategies to circumvent such growth defects without loosing the improved stress tolerance. First, we introduced into tobacco a double construct carrying the genes TPS1 and TPS2 (encoding TPP) from Saccharomyces cerevisiae. Both genes are regulated by an Arabidopsis RuBisCO promoter from gene AtRbcS1A giving constitutive production of both enzymes. The second strategy involved stress-induced expression by fusing the coding region of ScTPS1 downstream of the drought-inducible Arabidopsis AtRAB18 promoter. In transgenic tobacco plants harbouring genetic constructs with either ScTPS1 alone, or with ScTPS1 and ScTPS2 combined, trehalose biosynthesis was turned on only when the plants experienced stress. The third strategy involved the use of AtRbcS]A promoter together with a transit peptide in front of the coding sequence of ScTPS1, which directed the enzyme to the chloroplasts. This paper confirms that the enhanced drought tolerance depends on unknown ameliorated water retention as the initial water status is the same in control and transgenic plants and demonstrates the influence of expression of heterologous trehalose biosynthesis genes on Arabidopsis root development.
|
|
| 2. |
- Adcox, K, et al.
(författare)
-
PHENIX detector overview
- 2003
-
Ingår i: Nuclear Instruments & Methods in Physics Research. Section A: Accelerators, Spectrometers, Detectors, and Associated Equipment. - 0167-5087. ; 499:2-3, s. 469-479
-
Tidskriftsartikel (refereegranskat)abstract
- The PHENIX detector is designed to perform a broad study of A-A, p-A, and p-p collisions to investigate nuclear matter under extreme conditions. A wide variety of probes, sensitive to all timescales, are used to study systematic variations with species and energy as well as to measure the spin structure of the nucleon. Designing for the needs of the heavy-ion and polarized-proton programs has produced a detector with unparalleled capabilities. PHENIX measures electron and muon pairs, photons, and hadrons with excellent energy and momentum resolution. The detector consists of a large number of subsystems that are discussed in other papers in this volume. The overall design parameters of the detector are presented. (C) 2002 Elsevier Science B.V. All rights reserved.
|
|
| 3. |
- Andersson, Marica, et al.
(författare)
-
Low Holding Densities Increase Stress Response and Aggression in Zebrafish
- 2022
-
Ingår i: Biology. - : MDPI AG. - 2079-7737. ; 11:5
-
Tidskriftsartikel (refereegranskat)abstract
- Simple Summary Zebrafish are used as experimental animals in labs all around the world. To ensure that the health of zebrafish is maintained at the highest level, it is important to know the optimal housing conditions of the animals, including the housing density. Guidelines for housing densities of zebrafish can then be spread and followed globally, making it possible to compare research data from different facilities. To investigate the optimal housing densities of zebrafish and to better understand how holding density affects zebrafish behaviour and physiology, we evaluated the welfare of zebrafish housed at different densities for nine weeks. We observed that fish housed at the lowest holding density of 1 fish/L stood out from the rest of the experimental fish, showing higher levels of aggression, secreting more of the stress hormone cortisol in the water, and spending more time in the top zone of the tank, possibly reflecting the fact that fish in this density were hiding more behind the floating plants. Our data indicate that zebrafish should not be kept at 1 fish/L, or lower, to ensure good welfare of the animals. With laboratory zebrafish (Danio rerio) being an established and popular research model, there is a need for universal, research-based husbandry guidelines for this species, since guidelines can help promote good welfare through providing appropriate care. Despite the widespread use of zebrafish in research, it remains unclear how holding densities affect their welfare. Previous studies have mainly evaluated the effects of holding densities on a single parameter, such as growth, reproductive output, or social interactions, rather than looking at multiple welfare parameters simultaneously. Here we investigated how chronic (nine weeks) exposure to five different holding densities (1, 4, 8, 12, and 16 fish/L) affected multiple welfare indicators. We found that fish in the 1 fish/L density treatment had higher free water cortisol concentrations per fish, increased vertical distribution, and displayed aggressive behaviour more frequently than fish held at higher densities. On the other hand, density treatments had no effect on anxiety behaviour, whole-brain neurotransmitter levels, egg volume, or the proportion of fertilised eggs. Our results demonstrate that zebrafish can be held at densities between 4 and 16 fish/L without compromising their welfare. However, housing zebrafish in the density of 1 fish/L increased their stress level and aggressive behaviour.
|
|
| 4. |
- Bergström, Mattias, et al.
(författare)
-
Needs as a basis for design rationale
- 2008
-
Konferensbidrag (refereegranskat)abstract
- A basic principle for Needfinding [Faste, 1987; Patnaik & Becker, 1999] is that designers and engineers should interact directly with users to get direct insights into the user domain. Needfinding is not a new phenomena, it is almost forty years ago since the process was adopted at Stanford University’s product design program [Patnaik & Becker, 1999]. As the name, Need-finding, implies, this is an intertwined approach to find needs which are not readily articulated by users. The application of a Needfinding process offers qualitative methods to make those needs visible early on in product development. In fact, the process has become more interesting during recent time, since qualitative methods have gained more acceptance outside the academic realm [ibid.].The word qualitative indicates that what are sought for are qualities such as people’s experiences, what they perceive or interpret into a situation [Miles & Huberman, 1994; Patton, 2002]. Such data is contextually dependent, i.e., it must be generated in the context in which the phenomena occur. Besides context, people’s activities, behaviours and goals are important to observe and learn from. The objectives, for applying Needfinding, are to make the identification of needs and design a seamless effort, as well as an interest to identify opportunities to innovations. Needs last longer than any solution [Patnaik & Becker, 1999], since they are grounded in people’s activities. The solution and product that might meet such needs change over time. One example is how to store computer data, the products which satisfy the need has changed from, e.g., punch cards, magnetic tape, floppy discs [ibid.] to USB-flash memories. A guiding methodology in Needfinding is a flexible process, which is adapted to the task at hand [Kelley, 2001]. Such a process is conveyed in a few basic steps and, builds on a ‘philosophy’ which permeates all activities in order to adapt the process according to the project. Therefore, the designer’s ability to rely on such a process depends on familiarity with a number of methods for observations and interviews, as well as an aptitude for socio-technical skills. Hence, the purpose in this paper is to present and reflect on methods used in a running development project to identify needs in a product development project. This is done to contribute to the advancement of a need driven product development process. The disposition of this paper is as follows. First, our approach in studying the need identification activities is presented. Second, a theoretical frame for need identification and design is presented, i.e., Needfinding [Patnaik & Becker, 1999]. Third, the practice of finding needs is outlined and discussed.
|
|
| 5. |
- Bergström, Mattias, et al.
(författare)
-
Needs as a basis for design rationale
- 2008
-
Ingår i: Design 2008. - Zagreb : University of Zagreb. - 9789536313891 ; , s. 281-288
-
Konferensbidrag (refereegranskat)abstract
- This study is based on data from a Swedish real-life industrial product development project for e-health care of elderly. The purpose in the paper is to discuss identification of user needs. Information about the elderly is transferred in recurrent meetings. Besides the perception that these meetings occupy time which could be spent giving care, the nurses find it problematic to convey such information to substitutes, as well as they have to rely on their memory. In this case, a Dictaphone device was a solution. Reports on practical activities of identifying user needs and how they affect decisions in product development are limited; one contribution of this paper is insights into such a case
|
|
| 6. |
|
|
| 7. |
- Björklund, Henrik, et al.
(författare)
-
Between a Rock and a Hard Place : Parsing for Hyperege Replacement DAG Grammars
- 2016
-
Ingår i: Proc. 10th International Conference on Language and Automata Theory and Applications (LATA 2016). - Cham : Springer Publishing Company. - 9783319300009 - 9783319299990 ; , s. 521-532
-
Konferensbidrag (refereegranskat)abstract
- Motivated by applications in natural language processing, we study the uniform membership problem for hyperedge-replacement grammars that generate directed acyclic graphs. Our major result is a low-degree polynomial-time algorithm that solves the uniform membership problem for a restricted type of such grammars. We motivate the necessity of the restrictions by two different NP-completeness results.
|
|
| 8. |
- Björklund, Henrik, et al.
(författare)
-
Minimisation and Characterisation of Order-Preserving DAG Grammars
- 2018
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Order-preserving DAG grammars (OPDGs) is a formalism for processing semantic infor- mation in natural languages [5, 4]. OPDGs are sufficiently expressive to model abstract meaning representations, a graph-based form of semantic representation in which nodes en- code objects and edges relations. At the same time, they allow for efficient parsing in the uniform setting, where both the grammar and subject graph are taken as part of the input.In this article, we introduce an initial algebra semantic for OPDGs, which allows us to view them as regular tree grammars. This makes it possible to transfer a number of results from that domain to OPDGs, both in the unweighted and the weighted case. In particular, we show that deterministic OPDGs can be minimised efficiently, and that they are learnable in the so-called MAT setting. To conclude, we show that the languages generated by OPDGs are MSO-definable.
|
|
| 9. |
- Björklund, Henrik, et al.
(författare)
-
On the Regularity and Learnability of Ordered DAG Languages
- 2017
-
Ingår i: Implementation and Application of Automata. - Cham : Springer International Publishing. - 9783319601335 - 9783319601342 ; , s. 27-39
-
Konferensbidrag (refereegranskat)abstract
- Order-Preserving DAG Grammars (OPDGs) is a subclass of Hyper-Edge Replacement Grammars that can be parsed in polynomial time. Their associated class of languages is known as Ordered DAG Lan- guages, and the graphs they generate are characterised by being acyclic, rooted, and having a natural order on their nodes. OPDGs are useful in natural-language processing to model abstract meaning representa- tions. We state and prove a Myhill-Nerode theorem for ordered DAG languages, and translate it into a MAT-learning algorithm for the same class. The algorithm infers a minimal OPDG G for the target language in time polynomial in G and the samples provided by the MAT oracle.
|
|
| 10. |
- Björklund, Henrik, et al.
(författare)
-
Parsing Weighted Order-Preserving Hyperedge Replacement Grammars
- 2019
-
Ingår i: Proceedings of the 16th Meeting on the Mathematics of Language. - : Association for Computational Linguistics. ; , s. 1-11
-
Konferensbidrag (refereegranskat)abstract
- We introduce a weighted extension of the recently proposed notion oforder-preserving hyperedge-replacement grammars and prove that the weightof a graph according to such a weighted graph grammar can be computeduniformly in quadratic time (under assumptions made precise in the paper).
|
|
