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- Ariöz, Candan, 1983-, et al.
(författare)
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Heterologous overexpression of a monotopic glucosyltransferase (MGS) induces fatty acid remodeling in Escherichia coli membranes :
- 2014
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Ingår i: Biochimica et Biophysica Acta - Biomembranes. - : Elsevier BV. - 0005-2736 .- 1879-2642. ; 1838:7, s. 1862-1870
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Tidskriftsartikel (refereegranskat)abstract
- The membrane protein monoglucosyldiacylglycerol synthase (MGS) from Acholeplasma laidlawii is responsible for the creation of intracellular membranes when overexpressed in Escherichia coli (E. coli). The present study investigates time dependent changes in composition and properties of E. coli membranes during 22 h of MGS induction. The lipid/protein ratio increased by 38% in MGS-expressing cells compared to control cells. Time-dependent screening of lipids during this period indicated differences in fatty acid modeling. (1) Unsaturation levels remained constant for MGS cells (~ 62%) but significantly decreased in control cells (from 61% to 36%). (2) Cyclopropanated fatty acid content was lower in MGS producing cells while control cells had an increased cyclopropanation activity. Among all lipids, phosphatidylethanolamine (PE) was detected to be the most affected species in terms of cyclopropanation. Higher levels of unsaturation, lowered cyclopropanation levels and decreased transcription of the gene for cyclopropane fatty acid synthase (CFA) all indicate the tendency of the MGS protein to force E. coli membranes to alter its usual fatty acid composition.
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- Ashfaq, Awais, 1990-, et al.
(författare)
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Data resource profile : Regional healthcare information platform in Halland, Sweden
- 2020
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Ingår i: International Journal of Epidemiology. - Oxford : Oxford University Press. - 0300-5771 .- 1464-3685. ; 49:3, s. 738-739f
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Tidskriftsartikel (refereegranskat)abstract
- Accurate and comprehensive healthcare data coupled with modern analytical tools can play a vital role in enabling care providers to make better-informed decisions, leading to effective and cost-efficient care delivery. This paper describes a novel strategic healthcare analysis and research platform that encapsulates 360-degree pseudo-anonymized data covering clinical, operational capacity and financial data on over 500,000 patients treated since 2009 across all care delivery units in the county of Halland, Sweden. The over-arching goal is to develop a comprehensive healthcare data infrastructure that captures complete care processes at individual, organizational and population levels. These longitudinal linked healthcare data are a valuable tool for research in a broad range of areas including health economy and process development using real world evidence.Key messagesStructured and standardized variables have been linked from different regional healthcare sources into a research information platform including all healthcare visits in the county of Halland in Sweden, from 2009 to date.Since 2015, the regional information platform integrates a cost component to each healthcare visit: thus being able to quantify patient level value, safety and cost efficiency across the continuum of care. © The Author(s) 2020; all rights reserved. Published by Oxford University Press on behalf of the International Epidemiological Association
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- Athley, Fredrik, 1968, et al.
(författare)
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High-Resolution Space-Time Signal Processing for Radar
- 2003
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Ingår i: High-Resolution and Robust Signal Processing, To be published, Dekker, editor Y. Hua and A. Gershman and Q. Cheng. - : CRC Press. - 9781482276404
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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- Awad, Doaa, et al.
(författare)
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Halogenated Dodecaborate Clusters as Agents to Trigger Release of Liposomal Contents
- 2015
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Ingår i: ChemPlusChem. - : Wiley. - 2192-6506. ; 80:4, s. 656-664
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Tidskriftsartikel (refereegranskat)abstract
- Halogenated dodecaborates, and especially dodecaiodododecaborate(2−), are found to trigger effectively the release of the contents of phospholipid liposomes, including liposomes containing distearoylphosphatidylcholine and cholesterol, which are used clinically in cancer therapy. The basis of the release is studied through differential scanning calorimetry, cryo-transmission electron microscopy, and atomic force microscopy. Upon administration at high concentrations, drastic morphological changes are induced by the dodecaborates. Their possible use in triggered release is suggested.
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- Balksten, Kristin, 1977-, et al.
(författare)
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Lime burning tradition in field kilns : a case study of the Jämtland tradition in Sweden
- 2013
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Konferensbidrag (refereegranskat)abstract
- This study focuses on the local lime tradition in the region of Jämtland, in central Sweden. Local lime was used when building the medieval stone churches and since they are in a need of restoration there is subsequently a need for understanding the use of local lime. The geology of Jämtland contains several layers of limestone in the folded mountains. There is a broad spectrum ranging from pure Silurian limestone to clay containing Ordovician limestone, giving all kinds of lime from pure air lime to strong hydraulic lime. The preserved historic mortarshave mostly been made with the hydraulic lime. Several old field kilns have been preserved in the forest landscape as prehistoric monuments, showing the model of the local lime burning tradition. This paper discusses the process of identifying the historic lime kiln constructions and their burning technique. It also describes the process of slaking this hydraulic binder in order to produce a lime mortar with workability and compatibility required from a restoration mortar. Newly-produced samples of lime mortar have been compared with historic ones in thin section microscope for further understanding.
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- Balksten, Kristin, et al.
(författare)
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Lime burning tradition in field kilns – a case study of the Jämtland tradition in Sweden
- 2013
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Ingår i: 3rd Historic Mortars Conference11-14 September 2013, Glasgow, Scotland.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Abstract To be able to restore the medieval churches in the northern part of Sweden with a compatible traditional lime mortar there is a need for understanding the local lime tradition of the region of Jämtland. The geology of Jämt-land contains several layers of lime stone in the folded mountains. There is a broad spectrum from pure Silurian lime stone to clay containing Ordovician lime stone giving all kinds of lime from pure air lime to strong hydraulic lime. The historic mortars found have mainly been made from the strong hydraulic ones. Several old field kilns have been preserved in the forest landscape as prehistoric monuments, showing the model of the local lime burning tradition. This paper describes the process to identify the historic lime kiln constructions and their burn-ing technique. It also describes the process of slaking this hydraulic mortar in order to receive a lime mortar with workability and compatibility in order to find a restoration mortar. The new lime mortars have been compared with the historic ones in thin section microscopy for further understanding. Key words: lime mortar, slaked lime, lime burning, lime kiln, workability
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- Bello, Gianluca, et al.
(författare)
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Characterization of the aggregates formed by various bacterial lipopolysaccharides in solution and upon interaction with antimicrobial peptides
- 2015
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Ingår i: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 31:2, s. 741-751
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Tidskriftsartikel (refereegranskat)abstract
- The biophysical analysis of the aggregates formed by different chemotypes of bacterial lipopolysaccharides (LPS) before and after challenge by two different anti-endotoxic antimicrobial peptides (LL37 and bovine lactoferricin), was performed in order to determine their effect on the morphology of LPS aggregates. Small-angle neutron scattering (SANS) and cryogenic transmission electron microscopy (cryoTEM) were used to examine the structures formed by both smooth and rough LPS chemotypes and the effect of the peptides, by visualization of the aggregates and analysis of the scattering data by means of both mathematical approximations and defined models. The data showed that the structure of LPS determines the morphology of the aggregates and inuences the binding activity of both peptides. The morphologies of the worm-like micellar aggregates formed by the smooth LPS were relatively unaltered by the presence of the peptides due to their pre-existing high degree of positive curvature being little affected by their association with either peptide. On the other hand the aggregates formed by the rough LPS chemotypes, showed marked morphological changes from lamellar structures to ordered micellar networks, induced by the increase in positive curvature engendered upon association with the peptides. The combined use of cryoTEM and SANS proved to be a very useful tool for studying the aggregation properties of LPS in solution at biologically relevant concentrations.
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