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| 2. |
- D'Elia, Domenica, et al.
(författare)
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The 20th anniversary of EMBnet : 20 years of bioinformatics for the Life Sciences community
- 2009
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Ingår i: BMC Bioinformatics. - 1471-2105. ; 10:Suppl. 6, s. S1-
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Tidskriftsartikel (refereegranskat)abstract
- The EMBnet Conference 2008, focusing on 'Leading Applications and Technologies in Bioinformatics', was organized by the European Molecular Biology network (EMBnet) to celebrate its 20th anniversary. Since its foundation in 1988, EMBnet has been working to promote collaborative development of bioinformatics services and tools to serve the European community of molecular biology laboratories. This conference was the first meeting organized by the network that was open to the international scientific community outside EMBnet. The conference covered a broad range of research topics in bioinformatics with a main focus on new achievements and trends in emerging technologies supporting genomics, transcriptomics and proteomics analyses such as high-throughput sequencing and data managing, text and data-mining, ontologies and Grid technologies. Papers selected for publication, in this supplement to BMC Bioinformatics, cover a broad range of the topics treated, providing also an overview of the main bioinformatics research fields that the EMBnet community is involved in.
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| 3. |
- Eriksson, Nils-Einar, et al.
(författare)
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A voltage clamp circuit for measuring rapid current transients in membranes
- 1976
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Ingår i: Medical and biological engineering. - 0025-696X .- 1741-0444. ; 14:3, s. 334-338
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Tidskriftsartikel (refereegranskat)abstract
- A voltage clamp circuit is described which permits the measurements of current transients in membranes with time constants of the order of microseconds. The resistance Rs in series with the membrane is compensated for by a current feedback circuit which produces a rapid charging of the membrane capacitance C. It is possible by this arrangement to reduce the charging period to a value considerably shorter than the time constant RsC.
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| 4. |
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| 5. |
- Persson, A E, et al.
(författare)
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The hydraulic conductivity of the rat proximal tubular wall determined with colloidal solutions
- 1975
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Ingår i: Pflügers Archiv. - 0031-6768 .- 1432-2013. ; 360:1, s. 25-44
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Tidskriftsartikel (refereegranskat)abstract
- The hydraulic conductivity of the rat proximal tubular wall was determined using colloidal solutions perfused in short (50--200 mum) (SMP) or long (90--200 mum) (LMP) proximal tubular segments. In SMP human serum albumin (HSA) or polyvinylpyrrolidone (PVP) was added to raffinose solutions. A Lp of 0.019 nl-min-1-mm-1-mm Hg-1 was found when high colloid concentrations were used while values of 0.055--0.092 were found when low colloid concentrations were used. In other experiments, the Lp was determined by perfusing short tubular segments with pure raffinose solutions. A value of 0.015 nl-min-1-mm-1-mm Hg-1 was found. This is twice the value found when raffinose solutions were perfused through long tubular segments and it is concluded that the short microperfusion technique overestimates Lp with a factor of two. When microperfusions of long tubular segments were conducted, PVP was added to an equilibrium solution consisting of NaCl (110 mM) and raffinose (80 mM). Lp was found to be 0.018--0.021 when high colloid concentrations were used, while a value of 0.029 was found when a low colloid concentration was used. As found in both SMP and LMP a decrease in Lp's with increasing colloid concentrations indicates that a significant influence of radial concentration differences is highly probable. It is therefore suggested that the highest Lp derived when using the lowest colloid concentrations represents the best estimate. With this Lp value (0.03--0.05 nl-min-1-mm-1-mm Hg-1) and the existing transtubular hydrostatic and oncotic pressure difference it can be calculated that these passive forces might constitute the driving force for 1/3 of the fluid reabsorbed in the proximal tubule.
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| 6. |
- Sandblom, John, et al.
(författare)
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Electrical relaxation processes in black lipid membranes in the presence of a cation-selective ionophore
- 1975
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Ingår i: Journal of Membrane Biology. - 0022-2631 .- 1432-1424. ; 23:1, s. 1-19
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Tidskriftsartikel (refereegranskat)abstract
- The time course of relaxation of the electric current following steps in the applied potential across lipid bilayer membranes has been measured. The membranes were made cation-selective by the addition of nonactin. To permit the measurement of very short time constants a voltage clamp device was developed in order to reduce the charging period to less than 1 musec, regardless of the magnitude of the series resistances in the external solutions. It was possible by this method to establish the presence of two electric processes, which were found to behave differently with respect to temperature, applied potential and external solution conditions. The rapid process (tau approximately 10 musec) was interpreted in terms of the electric parameters of the polar part of the membrane according to the theory developed by Hägglund and Sandblom (T.I.T.J. Life Sic. 2: 107, 1972). The second process (tau approximately 100 musec) showed a behavior consistent with the model of Stark, Ketterer, Benz and Läuger (Biophys. J. 11:981, 1971), which considers the different rate constants involved in the net transfer of carriermediated ion transport across bilayer membranes.
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| 7. |
- Sperber, Göran, 1944-, et al.
(författare)
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RetroTector online, a rational tool for analysis of retroviral elements in small and medium size vertebrate genomic sequences
- 2009
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Ingår i: BMC Bioinformatics. - 1471-2105. ; 10 Suppl 6, s. S4-
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: The rapid accumulation of genomic information in databases necessitates rapid and specific algorithms for extracting biologically meaningful information. More or less complete retroviral sequences, also called proviral or endogenous retroviral sequences; ERVs, constitutes at least 5% of vertebrate genomes. After infecting the host, these retroviruses have integrated in germ line cells, and have then been carried in genomes for at least several 100 million years. A better understanding of structure and function of these sequences can have profound biological and medical consequences. METHODS: RetroTector (ReTe) is a platform-independent Java program for identification and characterization of proviral sequences in vertebrate genomes. The full ReTe requires a local installation with a MySQL database. Although not overly complicated, the installation may take some time. A "light" version of ReTe, (RetroTector online; ROL) which does not require specific installation procedures is provided, via the World Wide Web. RESULT: ROL http://www.fysiologi.neuro.uu.se/jbgs/ was implemented under the Batchelor web interface (A Lövgren et al). It allows both GenBank accession number, file and FASTA cut-and-paste admission of sequences (5 to 10,000 kilobases). Up to ten submissions can be done simultaneously, allowing batch analysis of
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