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- Bremer, B., K, Bremer, N. Heidari, P. Erixon, R. G. Olmstead, A. A. Anderberg, M. Källersjö, & E. Borkhardarian
(författare)
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Phylogenetics of asterids based on 3 coding and 3 non-coding chloroplast DNA markers and the utility of non-coding DNA at higher taxonomic level
- 2002
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Ingår i: Molecular Phylogenetics and Evolution. ; 24, s. 273-300.
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Tidskriftsartikel (refereegranskat)
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- Jansen, Jacob G., et al.
(författare)
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Separate Domains of Rev1 Mediate Two Modes of DNA Damage Bypass in Mammalian Cells
- 2009
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Ingår i: Molecular and Cellular Biology. - 0270-7306 .- 1098-5549. ; 29:11, s. 3113-3123
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Tidskriftsartikel (refereegranskat)abstract
- The Y family DNA polymerase Rev1 has been proposed to play a regulatory role in the replication of damaged templates. To elucidate the mechanism by which Rev1 promotes DNA damage bypass, we have analyzed the progression of replication on UV light-damaged DNA in mouse embryonic fibroblasts that contain a defined deletion in the N-terminal BRCT domain of Rev1 or that are deficient for Rev1. We provide evidence that Rev1 plays a coordinating role in two modes of DNA damage bypass, i.e., an early and a late pathway. The cells carrying the deletion in the BRCT domain are deficient for the early pathway, reflecting a role of the BRCT domain of Rev1 in mutagenic translesion synthesis. Rev1-deficient cells display a defect in both modes of DNA damage bypass. Despite the persistent defect in the late replicational bypass of fork-blocking (6-4) pyrimidine-pyrimidone photoproducts, overall replication is not strongly affected by Rev1 deficiency. This results in almost completely replicated templates that contain gaps encompassing the photoproducts. These gaps are inducers of DNA damage signaling leading to an irreversible G(2) arrest. Our results corroborate a model in which Rev1-mediated DNA damage bypass at postreplicative gaps quenches irreversible DNA damage responses.
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- Nilsson, K. -F, et al.
(författare)
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A probabilistic methodology to determine failure probabilities and acceptance criteria for the KBS-3 inserts under ice-age load conditions
- 2006
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Ingår i: Proc. 11th Int. High Lvl, Radioactive Waste Managem. Conf. IHLRWM. - 0894486918 - 9780894486913 ; , s. 207-214
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Konferensbidrag (refereegranskat)abstract
- The Swedish KBS-3 copper-cast iron canister for geological disposal is in an advanced stage. This reports deals with the cast iron insert that provides the mechanical strength of the canister and outlines an approach to assess the failure probabilities at large isostatic pressure (44 MPa) for manufactured canisters and how to derive acceptance criteria. The work includes a statistical test programme using three inserts for the tensile, compression and fracture properties. Specimens used for material characterization were also investigated by micro-structural analysis to determine the microstructure and to classify and size defects. It was found that the material scatter and low ductility was caused by many defect types, but with slag defects in the form of oxidation films as the most important one. These data were then used to compute defect distributions and as direct input to FE-calculations of KBS-3 canisters. A large number of FE-analyses were performed at the maximum design load (44 MPa) covering distributions of material parameters and geometrical features of the canisters. The computed probabilities for fracture and plastic collapse were very low even for material data with poor ductility. Two large scale isostatic compression tests of KBS-3 mock-ups to assess safety margins are also described. The failure occurred at loads above 130 MPa in both cases, indicating a safety margin of at least a factor three against the maximum design load.
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