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  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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  • Zhang, Wei-Min, et al. (författare)
  • Synthesis of mesoporous silica by a surface charge reversal route
  • 2010
  • Ingår i: Journal of Colloid and Interface Science. - : Elsevier BV. - 0021-9797 .- 1095-7103. ; 349:2, s. 473-476
  • Tidskriftsartikel (refereegranskat)abstract
    • Pore size adjustable mesoporous silica was synthesized by adsorption of varying amount of sodium dodecyl benzenesulfonate at the surface of silica activated by zinc ion via a novel surface charge reversal route. The pore size and volume can be adjusted from 5.9 to 13.76 nm and 0.88 to 1.08 cm3·g-1 respectively with increasing the SDBS concentration from 0.77 to 3.08 mmol·L-1. Adsorption of Zn2+ as a function of pH and N2 adsorption/desorption isotherms demonstrated that the metal ions such as Zn2+ could be readily removed with dilute nitric acid without apparent collapse of the pore structure at the proper range of SDBS concentration.
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