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- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
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| 3. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 4. |
- Ding, Linfang, et al.
(författare)
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Integrating 3D city data through knowledge graphs
- 2024
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Ingår i: Geo-spatial Information Science. - : Taylor & Francis. - 1009-5020 .- 1993-5153.
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Tidskriftsartikel (refereegranskat)abstract
- CityGML is a widely adopted standard for representing and exchanging 3D city models. The representation of semantic and topological properties in CityGML makes it possible to query such 3D city data for analysis in various applications. Nevertheless, the potential of querying CityGML data has not been fully exploited. The official GML encoding of CityGML is mainly an information model used for data storage and exchange, but not suitable for performing complex queries. The most common way of dealing with CityGML data is to store them as tables in the 3DCityDB system. However, it remains a challenging task for end users to formulate SQL queries over 3DCityDB directly for their ad-hoc analytical tasks because of the gap between the semantics of CityGML and the relational schema adopted in 3DCityDB. The technology of Knowledge Graphs (KGs), where an ontology is at the core, is a good solution to bridge such a gap. Moreover, embracing KGs makes it easier to integrate with other spatial data sources, e.g. OpenStreetMap, and to perform queries combining information from multiple data sources. In this work, we describe a CityGML-KG framework to expose the CityGML data in 3DCityDB as a KG. To evaluate our approach, we use CityGML data from the city of Munich as a test area and integrate OpenStreetMap data.
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| 5. |
- Li, Dongsheng, et al.
(författare)
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A highly conductive bimodal isotropic conductive adhesive and its reliability
- 2011
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Ingår i: ECS Transactions. ; 34:1, s. 583 - 588
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Konferensbidrag (refereegranskat)abstract
- In this paper, micro silver flakes and micro spherical particles were incorporated into the matrix resin of isotropic conductive adhesives (ICAs). Their electrical properties were investigated. The total weight ratio of silver fillers was kept at 75 wt% for all samples. When the content of micro spherical particles was 8 wt%, the bulk resistivity of the bimodal ICAs reduced dramatically to as low as 1.26×10-4 Ω.cm and its viscosity was 24,289 cP under 5rpm at 25°. Scanning electronic microscopy (SEM) images of the bimodal ICAs showed silver fillers well distributed in the matrix resin. In addition, the lap shear strength of different metal surfaces, and the bulk resistivity shifts during aging time under85°/85% RH for more than 500 hours were also measured. The results showed that bulk resistivity shifts of bimodal ICAs remained stable after further cured and the bond strength on the copper surface was the greatest among the three metal surfaces tested.
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