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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Yi, Peng, et al. (författare)
  • Late Holocene pathway of Asian Summer Monsoons imprinted in soils and societal implications
  • 2019
  • Ingår i: Quaternary Science Reviews. - : Elsevier. - 0277-3791 .- 1873-457X. ; 215, s. 35-44
  • Tidskriftsartikel (refereegranskat)abstract
    • The Asian Summer Monsoons (ASM) represent the main source of precipitation in China and East Asia with about one third of the world population and a region of widespread civilizations. Identifying the temporal and spatial patterns (pathways) of these monsoonal events during the Late Holocene to today has been a matter of debate amongst the scientific community. Here we show that the distribution patterns of the cosmogenic isotope Be-10 and oceanic I-127 in the topsoil across China exhibit imprints of the main ASM pathways. Our results indicate the monsoon pathway pattern persisted for several millennia or more and suggest a strong bond between Be-10 and water vapor transport patterns. Our data also reveal a(127)I distribution pattern controlled by the ASM pathways, rather than proximity to the sea or bedrock weathering. The persistent pathway of the ASM during the late Holocene, together with higher than average global soil iodine concentration, may have further strengthened the development of civilizations in this region of the world through reduction of iodine deficiency related diseases. (C) 2019 Elsevier Ltd. All rights reserved.
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