| 1. |
- Adams, JC, et al.
(författare)
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Characterisation of Drosophila thrombospondin defines an early origin of pentameric thrombospondins
- 2003
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Ingår i: Journal of Molecular Biology. - 1089-8638. ; 328:2, s. 479-494
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Tidskriftsartikel (refereegranskat)abstract
- Thrombospondins (TSPs) are multidomain oligomers that have complex roles in cell interactions and tissue organisation. The five vertebrate TSPs comprise two subgroups, A and B, that are assembled as trimers or pentamers, respectively. An invertebrate TSP was recently discovered in Drosophila melanogaster, but there is no knowledge of the oligomerisation status or properties of this molecule. We developed by bioinformatics a new dataset containing the single TSP of Drosophila melanogaster and four other newly identified invertebrate TSPs to examine the phylogenetic relationships of TSPs. These analyses clearly indicate pentamerisation as an early attribute of TSPs. We demonstrate experimentally that D. melanogaster TSP is assembled as a pentamer, has heparin-binding activity and is a component of extracellular matrix (ECM). During embryogenesis, the TSP transcript is concentrated at muscle attachment sites and is expressed by a subset of myoblasts and in imaginal discs. These novel results establish TSPs as highly conserved ECM components in both invertebrates and vertebrates and open fresh perspectives on the conservation of structure and biological function within this family
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| 2. |
- Fascetti, Nora, et al.
(författare)
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Expression of Drosophila Ten-a, a dimeric receptor during embryonic development.
- 2002
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Ingår i: Mechanisms of Development. - 0925-4773. ; 114:1-2, s. 197-200
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Tidskriftsartikel (refereegranskat)abstract
- Ten-a and Ten-m are the two Drosophila members of the newly discovered Ten-m family of dimeric type II transmembrane proteins. Here, we report complete cDNA cloning and protein expression patterns of Ten-a. The Ten-a protein, a dimeric receptor of about 500 kDa is mainly expressed on axons of the embryonic central nervous system and on muscle attachment sites.
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| 3. |
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| 4. |
- Khare, Narmada, et al.
(författare)
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Expression patterns of two new members of the Semaphorin family in Drosophila suggest early functions during embryogenesis
- 2000
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Ingår i: Mechanisms of Development. - 0925-4773. ; 91:08-01-02, s. 393-397
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Tidskriftsartikel (refereegranskat)abstract
- We report the sequence and expression analysis of two new Drosophila members of the Semaphorin family. Both proteins show the presence of Semaphorin domains and transmembrane domains. Both genes are expressed maternally and in embryos, and reveal distinct expression patterns much earlier than the onset of neurogenesis. We also present an overview of the domain structure of all so far known semaphorins in Drosophila. Furthermore, we compared all Drosophila and C. elegans Semaphorins and discuss them in the light of their evolution.
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| 5. |
- Ngernsiri, Lertluk, et al.
(författare)
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Preliminary studies on the role of ten-a during CNS and eye development of Drosophila.
- 2005
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Ingår i: ScienceAsia. - 1513-1874. ; 31, s. 5-11
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Tidskriftsartikel (refereegranskat)abstract
- ten-a is one of the two Drosophila genes whose products belong to the Ten M protein family. TheTen-a protein is a type II transmembrane protein. This protein was found to be mainly expressed in the CNSof the embryo and in the compound eye of the pupa. No report about the function of ten-a has beenpublished to date. Here, we showed, in a preliminary report, that ten-a has a function in the development ofthe CNS and in the compound eye. To study the function of ten-a during CNS development, doublestranded RNA interference (dsRNAi) experiments were performed. We found that embryos without Ten-aprotein have defects in the commissures, suggesting that ten-a is likely to be involved in commissureformation. Furthermore, to study the function of ten-a in the eye, the Gal4/UAS system was used tooverexpress ten-a at different stages of eye development. We found that extra amounts of Ten-a can interferewith the development of the eye. Flies overexpressing ten-a showed eye defects such as small and rough eyes,and black ommatidia. The results indicated that ten-a may be a novel gene involved in eye morphogenesis
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| 6. |
- Ngernsiri, Lertluk, et al.
(författare)
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Ten-a overexpression causes abnormal pattern in the Drosophila compound eye
- 2006
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Ingår i: Insect science. - : Wiley. - 1672-9609 .- 1744-7917. ; 13:5, s. 355-364
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Tidskriftsartikel (refereegranskat)abstract
- Abstract Ten-a is one of the two Drosophila proteins that belong to the Ten M protein family. This protein is a type II transmembrane protein and is expressed mainly in the embryonic CNS, in the larval eye imaginal disc and in the compound eye of the pupa. Here, we investigate the role of ten-a during development of the compound eye by using the Gal4/UAS system to induce ten-a overexpression in the developing eye. We found that overexpression of ten-a can perturb eye development during all stages examined. In an early stage, overexpression of ten-a in eye primordial cells caused small and rough eyes and interfered with photoreceptor cell recruitment, resulting in some ommatidia having fewer or extra photoreceptor cells. Conversely, ten-a overexpression during ommatidial formation caused severe eye defects due to absence of many cellular components. Interestingly, overexpression of ten-a in the late stage developing ommatidial cluster affected the number of pigment cells, caused cone cells proliferation in many ommatidia, and caused some photoreceptor cell defects. These results suggest that ten-a may be a novel gene required for normal eye morphogenesis.
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