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Sökning: WFRF:(Fearnley M)

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  • Burger, N., et al. (författare)
  • ND3 Cys39 in complex I is exposed mitochondrial
  • 2022
  • Ingår i: Cell Chemical Biology. - : Elsevier BV. - 2451-9448 .- 2451-9456. ; 29:4, s. 636-
  • Tidskriftsartikel (refereegranskat)abstract
    • Mammalian complex I can adopt catalytically active (A-) or deactive (D-) states. A defining feature of the reversible transition between these two defined states is thought to be exposure of the ND3 subunit Cys39 residue in the D-state and its occlusion in the A-state. As the catalytic A/D transition is important in health and disease, we set out to quantify it by measuring Cys39 exposure using isotopic labeling and mass spectrometry, in parallel with complex I NADH/CoQ oxidoreductase activity. To our surprise, we found significant Cys39 exposure during NADH/CoQ oxidoreductase activity. Furthermore, this activity was unaffected if Cys39 alkylation occurred during complex I-linked respiration. In contrast, alkylation of catalytically inactive complex I irreversibly blocked the reactivation of NADH/CoQ oxidoreductase activity by NADH. Thus, Cys39 of ND3 is exposed in complex I during mitochondrial respiration, with significant implications for our understanding of the A/D transition and the mechanism of complex I.
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  • Bertolotto, L, et al. (författare)
  • phi phi associated production in (p)over-bar-p annihilation from JETSET
  • 1996
  • Ingår i: PHYSICS OF ATOMIC NUCLEI. - : AMER INST PHYSICS. ; , s. 1444-1449
  • Konferensbidrag (refereegranskat)abstract
    • The JETSET (PS202) experiment at CERN has studied the reaction (p) over bar p --> 4K(+/-) in the invariant-mass range from 2.15 to 2.43 GeV/c(2). The phi phi, phi K+K-, and 4K(+/-) cross sections have been measured, and a spin-parity analysis of the phi p
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  • Resultat 1-5 av 5

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