| 1. |
- Perepelov, Andrei V., et al.
(author)
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Structure and genetics of the O-antigen of Escherichia coli O169 related to the O-antigen of Shigella boydii type 6
- 2015
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In: Carbohydrate Research. - : Elsevier BV. - 0008-6215 .- 1873-426X. ; 414, s. 46-50
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Journal article (peer-reviewed)abstract
- The O-polysaccharide (O-antigen) of Escherichia coli O169 was studied by sugar analysis along with 1D and 2D H-1 and C-13 NMR spectroscopy. The following structure of the branched hexasaccharide repeating unit was established: [GRAPHICS] The O-polysaccharide of E. coli O169 differs from that of Shigella boydii type 6 only in the presence of a side-chain glucose residue. A comparison of the O-antigen biosynthesis gene clusters between the galF to gnd genes in the genomes of the two bacteria revealed their close relationship. The glycosyltransferase gene responsible for the formation of the beta-D-Glcp-(1 -> 6)-alpha-D-Galp linkage in the O-antigen was identified in the gene cluster.
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| 2. |
- Perepelov, Andrei V., et al.
(author)
-
Structures and gene clusters of the closely related O-antigens of Escherichia coli O46 and O134, both containing D-glucuronoyl-D-allothreonine
- 2015
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In: Carbohydrate Research. - : Elsevier BV. - 0008-6215 .- 1873-426X. ; 409, s. 20-24
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Journal article (peer-reviewed)abstract
- The O-polysaccharides (O-antigens) were isolated by mild acid degradation of the lipopolysaccharide (LPS) of Escherichia coli O46 and O134. The structures of their linear tetrasaccharide repeating units were established by sugar analysis along with 1D and 2D H-1 and C-13 NMR spectroscopy: [GRAPHICS] where D-aThr indicates D-allothreonine and R indicates O-acetyl substitution (similar to 70% on aThr and similar to 15% on GalNAc) in E. coli O46 whereas the O-acetylation is absent in E. coli O134. Functions of genes in the essentially identical O-antigen gene clusters of E. coli O46 and O134 were tentatively assigned by a comparison with sequences in available databases and found to be in agreement with the O-polysaccharide structures established.
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| 3. |
- Kravchenko, T A, et al.
(author)
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Contribution of dimensional factor to the potential of copper-containing electron-ion exchangers
- 2006
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In: Russian journal of electrochemistry. - 1023-1935 .- 1608-3342. ; 42:3, s. 233-238
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Journal article (peer-reviewed)abstract
- The average size of copper particles in copper-containing electron-ion exchangers is determined with the aid of microscopy and x-ray diffractornetry. The results are used to calculate the contribution made by a dimensional factor to the electrode potential. Compared to the potential of compact copper, the potential shift in the negative direction measured experimentally falls within the region of calculated values. The average size of copper particles remains practically unchanged after contact with a copper sulfate solution. testifying to stabilization of an ultradisperse state of copper particles by a polymer matrix.
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