| 1. |
- Daehnick, W.W, et al.
(författare)
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Spin correlations in polarized p polarized p -> p n pi+ production near threshold
- 2002
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Ingår i: Physical Review C. Nuclear Physics. - : American Physical Society. - 0556-2813 .- 1089-490X. ; 65, s. 024003-1
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Tidskriftsartikel (refereegranskat)abstract
- A first measurement of longitudinell as well as transverse spin correlation coefficients for the reaction pp->pn pi+ is made using a polarized proton target and a polarized proton beam. We report kinematically complete measurements for this reaction at 32
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| 2. |
- Delroisse, J., et al.
(författare)
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A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence
- 2017
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Ingår i: Open Biology. - : The Royal Society. - 2046-2441. ; 7:4
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Tidskriftsartikel (refereegranskat)abstract
- Bioluminescence relies on the oxidation of a luciferin substrate catalysed by a luciferase enzyme. Luciferins and luciferases are generic terms used to describe a large variety of substrates and enzymes. Whereas luciferins can be shared by phylogenetically distant organisms which feed on organisms producing them, luciferases have been thought to be lineage-specific enzymes. Numerous light emission systems would then have co-emerged independently along the tree of life resulting in a plethora of non-homologous luciferases. Here, we identify for the first time a candidate luciferase of a luminous echinoderm, the ophiuroid Amphiura filiformis. Phylogenomic analyses identified the brittle star predicted luciferase as homologous to the luciferase of the sea pansy Renilla (Cnidaria), contradicting with the traditional viewpoint according to which luciferases would generally be of convergent origins. The similarity between the Renilla and Amphiura luciferases allowed us to detect the latter using anti-Renilla luciferase antibodies. Luciferase expression was specifically localized in the spines which were demonstrated to be the bioluminescent organs in vivo. However, enzymes homologous to the Renilla luciferase but unable to trigger light emission were also identified in non-luminous echinoderms and metazoans. Our findings strongly indicate that those enzymes, belonging to the haloalkane dehalogenase family, might then have been convergently co-opted into luciferases in cnidarians and echinoderms. In these two benthic suspension-feeding species, similar ecological pressures would constitute strong selective forces for the functional shift of these enzymes and the emergence of bioluminescence.
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| 3. |
- Delroisse, J., et al.
(författare)
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De novo transcriptome of the European brittle star Amphiura filiformis pluteus larvae
- 2015
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Ingår i: Marine Genomics. - : Elsevier. - 1874-7787. ; 23, s. 109-121
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Tidskriftsartikel (refereegranskat)abstract
- Background: In non-classical model species, Next Generation Sequencing increases the ability to analyze the expression of transcripts/genes. In this study, paired-end Illumina HiSeq sequencing technology has been employed to describe a larval transcriptome generated from 64 h post-fertilization pluteus larvae of the brittle star Amphiura filiformis. We focused our analysis on the detection of actors involved in the opsin based light perception, respectively the opsins and the phototransduction actors. Methods & results: In this research, about 47 million high quality readswere generated and 86,572 total unigenes were predicted after de novo assembly. Of all the larval unigenes, 18% show significant matches with reference online databases. 46% of annotated larval unigenes were significantly similar to transcripts from the purple sea urchin. COG, GO and KEGG analyses were performed on predicted unigenes. Regarding the opsin-based photoreception process, even if possible actors of ciliary and rhabdomeric phototransduction cascadeswere detected, no ciliary or rhabdomeric opsinwas identified in these larvae. Additionally, partial non-visual RGR (retinal G protein coupled receptor) opsin mRNAs were identified,possibly indicating the presence of visual cycle reaction in early pluteus larvae. The eye morphogene Pax 6 was also identified in the pluteus transcriptome. Conclusions: Contrary to sea-urchin larvae, brittle star larvae appear to be characterized by an absence of visuallike opsins. These RNA-seq data also provide a useful resource for the echinoderm research community and researchers with an interest in larval biology. © 2015 Elsevier B.V.
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| 4. |
- Delroisse, J, et al.
(författare)
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Opsin-based extraocular photoreception in a luminous brittle star
- 2014
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Ingår i: Luminescence. Special Issue: Abstracts of the 18th International Symposium on Bioluminescence and Chemiluminescence, June 23–28 2014, Uppsala, Sweden (ISBC 2014). - 1522-7235. ; 29:S1, s. 65-66
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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