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- Armitage, Emily G, et al.
(författare)
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Time-of-flight SIMS as a novel approach to unlocking the hypoxic properties of cancer
- 2013
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Ingår i: Surface and Interface Analysis. - 1096-9918 .- 0142-2421. ; 45:1, s. 282-285
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Tidskriftsartikel (refereegranskat)abstract
- It is known that hypoxia-inducible factor 1 (HIF-1) activity results in the coordinated up-regulation of a large number of proteins that facilitate cell survival in tumours; however, the effect of HIF-1 on cancer metabolism is less well characterised. With knowledge of the specific effect of HIF-1 on cancer metabolism, biomarkers could be identified for which new drugs could be targeted. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) offers the potential to analyse intact cells in situ and has a mass spectral coverage that is applicable to metabolic profiling. It has been used to analyse the effects of HIF-1 on multicellular tumour models. Multicellular tumour spheroids (MTSs) have been cultured from human colon carcinoma cells with and without the expression of HIF-1, and the surface of the cross sections of each MTS has been analysed. Because metabolic profiling is an emerging field in ToF-SIMS, there is a requirement to determine which metabolites can be detected using this technique and which of those can be identified in complex mixtures within biological samples. For this, a selection of metabolites have been analysed, and the ToF-SIMS standard spectra acquired have been used to localise metabolites in MTS sections. The comparison of metabolic profiles of MTSs with and without the expression of HIF-1 has elucidated potential biomarkers for tumour survival in hypoxia, some of which may be HIF-1 regulated.
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| 6. |
- Fletcher, John, 1978, et al.
(författare)
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C-60, buckminsterfullerene: its impact on biological ToF-SIMS analysis
- 2006
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Ingår i: Surface and Interface Analysis. - : Wiley. - 0142-2421 .- 1096-9918. ; 38:11, s. 1393-1400
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Forskningsöversikt (refereegranskat)abstract
- The desire to apply the analytical abilities of the time-of-flight secondary ion mass spectrometry (ToF-SIMS) experiment to increasingly diverse samples, particularly those of a biological nature, has provided the impetus for development of new technologies to overcome some of the inherent difficulties associated with such studies. Of these, one of the most important is the widespread introduction of polyatomic ion beams to increase the secondary ion yields of the higher mass, more chemically characteristic species. The introduction of the C-60 ion beam for routine analysis has made arguably the greatest impact, providing new possibilities for analysing molecular compounds present both on the surface and in some cases in the bulk of samples such as biological tissue sections and single cells. Copyright (C) 2006 John Wiley & Sons, Ltd.
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| 7. |
- Fletcher, John, 1978, et al.
(författare)
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Comparison of C60 and GCIB primary ion beams for the analysis of cancer cells and tumour sections
- 2013
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Ingår i: Surface and Interface Analysis. - 1096-9918 .- 0142-2421. ; 45:1, s. 273-276
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Tidskriftsartikel (refereegranskat)abstract
- We have implemented a gas cluster ion beam (GCIB) system developed by Ionoptika Ltd (Southampton, UK) with sufficient control to allow us to exploit the unique capabilities of our J105 instrument for imaging and depth profiling. The J105 allows us to use the GCIB as continuous primary ion beam, thereby overcoming the issues associated with pulsing these slow moving, mixed species beams. We have performed a direct comparison with C60 ions on the same samples in the same instrument. The GCIB beams are more difficult to focus than the C60+ ion beam, making single-cell imaging difficult, although spot sizes of 15–20 µm are readily obtainable for Ar1000 and Ar2000, providing good resolution for larger area imaging on tissue section/biopsy samples. In this paper, we present results from the assessment of these new beams as primary ions for the analysis of ‘real’, complex biological systems. Initial spectra and those following increased primary ion bombardment were compared for in vitro cultured cells deposited on silicon and cryo-sectioned tumour samples originating in vivo. Ar1000+ and Ar2000+ showed increased persistence of the signals from intact molecular ions of phospholipids and a reduction in the accumulation of chemical background noise compared with C60+ analysis.
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| 8. |
- Fletcher, John, 1978, et al.
(författare)
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DEVELOPMENTS IN MOLECULAR SIMS DEPTH PROFILING AND 3D IMAGING OF BIOLOGICAL SYSTEMS USING POLYATOMIC PRIMARY IONS
- 2011
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Ingår i: Mass Spectrometry Reviews. - : Wiley. - 0277-7037. ; 30:1, s. 142-174
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Forskningsöversikt (refereegranskat)abstract
- In principle mass spectral imaging has enormous potential for discovery applications in biology. The chemical specificity of mass spectrometry combined with spatial analysis capabilities of liquid metal cluster beams and the high yields of polyatomic ion beams should present unprecedented ability to spatially locate molecular chemistry in the 100 nm range. However, although metal cluster ion beams have greatly increased yields in the m/z range up to 1000, they still have to be operated under the static limit and even in most favorable cases maximum yields for molecular species from 1 mm pixels are frequently below 20 counts. However, some very impressive molecular imaging analysis has been accomplished under these conditions. Nevertheless although molecular ions of lipids have been detected and correlation with biology is obtained, signal levels are such that lateral resolution must be sacrificed to provide a sufficient signal to image. To obtain useful spatial resolution detection below 1 mm is almost impossible. Too few ions are generated! The review shows that the application of polyatomic primary ions with their low damage cross-sections offers hope of a new approach to molecular SIMS imaging by accessing voxels rather than pixels to thereby increase the dynamic signal range in 2D imaging and to extend the analysis to depth profiling and 3D imaging. Recent data on cells and tissue analysis suggest that there is, in consequence, the prospect that a wider chemistry might be accessible within a sub-micron area and as a function of depth. However, these advances are compromised by the pulsed nature of current ToF-SIMS instruments. The duty cycle is very low and results in excessive analysis times, and maximum mass resolution is incompatible with maximum spatial resolution. New instrumental directions are described that enable a dc primary beam to be used that promises to be able to take full advantage of all the capabilities of the polyatomic ion beam. Some new data are presented that suggest that the aspirations for these new instruments will be realized. However, although prospects are good, the review highlights the continuing challenges presented by the low ionization efficiency and the complications that arise from matrix effects. (C) 2010 Wiley Periodicals, Inc., Mass Spec Rev 30:142-174, 2011
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| 9. |
- Fletcher, John, 1978, et al.
(författare)
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Label free biochemical 2D and 3D imaging using secondary ion mass spectrometry.
- 2011
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Ingår i: Current opinion in chemical biology. - : Elsevier BV. - 1879-0402 .- 1367-5931. ; 15:5, s. 733-40
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Forskningsöversikt (refereegranskat)abstract
- Time-of-flight secondary ion mass spectrometry (ToF-SIMS) provides a method for the detection of native and exogenous compounds in biological samples on a cellular scale. Through the development of novel ion beams the amount of molecular signal available from the sample surface has been increased. Through the introduction of polyatomic ion beams, particularly C(60), ToF-SIMS can now be used to monitor molecular signals as a function of depth as the sample is eroded thus proving the ability to generate 3D molecular images. Here we describe how this new capability has led to the development of novel instrumentation for 3D molecular imaging while also highlighting the importance of sample preparation and discuss the challenges that still need to be overcome to maximise the impact of the technique.
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