| 1. |
- Cadena, Erasmo, et al.
(författare)
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Valorisation of Seafood Side-Streams through the Design of New Holistic Value Chains: WaSeaBi Project
- 2024
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Ingår i: Sustainability. - 2071-1050. ; 16:5
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Tidskriftsartikel (refereegranskat)abstract
- Growing demand in the fisheries sector has resulted in a high generation of side-streams that are mainly treated as waste despite their potential value in terms of protein, fatty acids, and minerals. The WaSeaBi project, funded by the EU under the Horizon 2020 BBI JU initiative, seeks to address this problem by promoting the sustainable and economically viable utilisation of these side-streams, thus contributing to improved food security and environmental conservation. The project focuses on the development of innovative technologies and methodologies for the efficient valorisation of seafood side-streams into marketable products such as protein-based food ingredients, bioactive peptides, and mineral supplements. The WaSeaBi project started with a comprehensive analysis to identify the bottlenecks hindering the efficient utilisation of side-streams. To this end, a comprehensive study of the European seafood industry was conducted to understand the existing challenges. The main obstacles identified were technological deficiencies, lack of space and personnel, and a limited market for the resulting products. Several laboratory-scale technologies, such as pH-shift, enzymatic hydrolysis, membrane concentration, and flocculation with centrifugation, were explored in order to extract valuable components from the side-streams. Subsequently, these technologies were scaled-up and tested on a pilot scale. For example, membrane concentration technology facilitated the recovery of valuable molecules from mussel cooking side-streams while reducing environmental impact. Flocculation helped recover proteins and phosphates from process waters, crucial for reducing the organic load of effluents. In addition, decision-making tools were developed to help select and build the most appropriate valorisation strategies, taking into account technical, legal, economic, and environmental aspects. Environmental sustainability was assessed through life cycle assessment, which highlighted the factors that contribute most to the environmental impact of each technology. The results revealed that reducing chemical consumption and improving energy efficiency are key to optimising the environmental performance of the valorisation technologies. The WaSeaBi project outlines a promising path towards sustainable and economically beneficial utilisation of seafood side-streams. By employing innovative technologies, the project not only contributes to reducing waste and environmental impact, but also facilitates the transformation of low-value side-streams into high-value products. In addition, it provides a structured framework to help industry stakeholders make informed decisions on the valorisation of secondary streams. This initiative marks a substantial step towards a more sustainable and economically viable fisheries and aquaculture industry, setting a precedent for future projects aimed at overcoming technological and infrastructural barriers to the valorisation of seafood side-streams.
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| 2. |
- Coque, Jorge, et al.
(författare)
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Recovery of Nutrients from Cod Processing Waters
- 2023
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Ingår i: Marine Drugs. - 1660-3397. ; 21:11
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Tidskriftsartikel (refereegranskat)abstract
- Liquid side-streams from food industries can be processed and used in food applications and contribute to reduce the environmental footprint of industries. The goal of this study was to evaluate the effectiveness and applicability of protein and phosphorus separation processes, namely microfiltration, ultrafiltration and flocculation, using protein-rich process waters with low (LS) and high (HS) salt content from the processing of salted cod (Gadus morhua). The application of different flocculants (chitosan lactate and Levasil RD442) were evaluated at different concentrations and maturation periods (0, 1 or 3 h). The results showed that different flocculation treatments resulted in different recoveries of the nutrients from LS and HS. Proteins in LS could be most efficiently recovered by using Levasil RD442 0.25% and no maturation period (51.4%), while phosphorus was most efficiently recovered when using Levasil RD442 1.23% and a maturation period of 1 h (34.7%). For HS, most of its protein was recovered using Levasil RD442 1.23% and a maturation period of 1 h (51.8%), while phosphorus was recovered the most using Levasil 1.23% and no maturation period (47.1%). The salt contents allowed interactions through intermolecular forces with Levasil RD442. The ultrafiltration method was effective on HS since it recovered higher percentages of nutrients in the retentate phase (57% of the protein and 46% of the phosphorus) compared to LS.
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| 3. |
- Forghani Targhi, Bita, 1973, et al.
(författare)
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Biochemical Characterization and Storage Stability of Process Waters from Industrial Shrimp Production
- 2021
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Ingår i: ACS Omega. - : American Chemical Society (ACS). - 2470-1343. ; 6:46, s. 30960-30970
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Tidskriftsartikel (refereegranskat)abstract
- Shrimp boiling water (SBW) and shrimp peeling water (SPW), generated during shrimp processing, were characterized in terms of crude composition, volatile compounds, as well as nutritional and potentially toxic elements over a 13 month sampling period. The storage stability of both waters was also evaluated. Results showed that SBW contained on median 14.8 g/L protein and 2.2 g/L total fatty acids with up to 50% comprising eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Astaxanthin esters, which dominated the total astaxanthin, were 2.8 mg/L on median. SPW, on the other hand, contained on median 1.0 g/L of protein, 0.21 g/L of total fatty acids, and 1.2 mg/L astaxanthin esters. For both side-streams, essential amino acids were up to 50% of total amino acids. For SBW and SPW, the most abundant nutritional elements were Na, K, P, Ca, Cu, and Zn. The contents of all potentially toxic elements were below the detection limits, except for As. SBW was more stable at 4 °C compared to SPW as shown, e.g., by thiobarbituric acid reactive substances and relative changes in total volatile basic nitrogen. The extensive compositional mapping of SBW/SPW provides crucial knowledge necessary in the exploitation and value-adding of such side-streams into food or feed products.
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| 4. |
- Forghani Targhi, Bita, 1973, et al.
(författare)
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Cultivation of microalgae- Chlorella sorokiniana and Auxenochlorella protothecoides- in shrimp boiling water residues
- 2022
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Ingår i: Algal Research. - : Elsevier BV. - 2211-9264. ; 65
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Tidskriftsartikel (refereegranskat)abstract
- Based on the ability of microalgae to purify industrial processing waters, the overall aim of this study was to evaluate whether currently wasted shrimp processing waters could be used as microalgal growth media to produce new protein-enriched food and feed ingredients. Low molecular weight (LMW) fractions of shrimp boiling water (SBW) which had been pre-flocculated using alginate (AL), carrageenan (CA), chitosan (CH) or Superfloc C-592 to recover shrimp protein via flotation, were used for cultivation of Chlorella sorokiniana and Auxenochlorella protothecoides to produce a protein-enriched microalgal biomass. CH-derived media induced the highest growth rates for both species with A. protothecoides out-performing C. sorokiniana. A. protothecoides best assimilated phosphate-phoshorous (P-PO4) and total phosphorous (TP) in all media; <63 mg/L and < 45 mg/L after 4 days, respectively. In upscaled aerated cultures of A. protothecoides in CH- and AL-derived media, P and TP uptake increased up to 85 and 127 mg/L, respectively. Further, 63% of the free amino acids (AA) were assimilated in both waters. Biomasses derived from SBW contained 37-43% protein and 15.0-17.4% fatty acids (FA) per DW; with 38-40% essential AA (EAA) and 21.3-22.5% polyunsaturated FA (PUFA), respectively Corresponding numbers for biomass cultivated in control media were 11 and 53%, protein and FA, respectively, and with 38% and 15.6% EAA and PUFA, respectively. Ability of A. protothecoides to assimilate TP and AA, and to generate a protein-rich biomass from LMW-fractions derived from SBW was thus revealed for the first time, and paves the way for a SBW-based biorefinery comprising chemical, physical and microbial processes to produce multiple products.
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| 5. |
- Forghani Targhi, Bita, 1973, et al.
(författare)
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Flocculation and Flotation to Recover Protein-Enriched Biomasses from Shrimp Boiling and Peeling Process Waters: A Case Study
- 2020
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Ingår i: ACS Sustainable Chemistry & Engineering. - : American Chemical Society (ACS). - 2168-0485. ; 8:26, s. 9660-9668
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Tidskriftsartikel (refereegranskat)abstract
- A novel integrated process for the recovery of protein-enriched biomasses from shrimp boiling water (SBW) and shrimp peeling water (SPW) was investigated by combining flocculation (F) and dissolved air flotation (DAF) into an F-DAF process. Alginate and carrageenan were used as flocculants. It was found that the protein yield from SPW and SBW in the F-DAF process was 68-97% and 26-45%, respectively. This led to a reduction in protein content of the influent SBW and SPW (12.4 and 1.4 g/L, respectively) by up to 76% and 85%, resulting in outlets with 2.9 and 0.2 g/L protein, respectively. Further, the F-DAF recovery process concentrated the proteins of SBW and SPW up to 7 and 29 times, respectively, thus, generating a protein-enriched biomass. After spray drying, biomass from SBW had up to 61% proteins and out of the total amino acids, up to 47% were essential ones. Further, the spray dried powder contained up to 23% total lipids, 2.7% long chained n-3 polyunsaturated fatty acids and 49.7 mg/g total astaxanthin. The studied F-DAF recovery system can thus be successfully used for recovering nutrients from process waters generated during the production of peeled shrimps.
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| 6. |
- Forghani Targhi, Bita, 1973, et al.
(författare)
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Liquid Side Streams from Mussel and Herring Processing as Sources of Potential Income
- 2023
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Ingår i: ACS Omega. - : American Chemical Society (ACS). - 2470-1343. ; 8:9, s. 8355-8365
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Tidskriftsartikel (refereegranskat)abstract
- The seafood industry generates significant amounts of process waters which can generate value upon recovery of their nutrients. Process waters from the herring marination chain and cooking of mussels were here characterized in terms of crude composition, volatile compounds, and nutritional and potentially toxic elements. Protein and total fatty acid contents of herring refrigerated sea water (RSW) reached 3 and 0.14 g/L, respectively, while herring presalting brine (13%) reached 16.3 g/L protein and 0.77 g/L total fatty acid. Among three herring marination brines vinegar brine (VMB), spice brine (SPB), and salt brine (SMB), SPB reached the highest protein (39 g/L) and fatty acids (3.0 g/L), whereas SMB and VMB at the most had 14 and 21 g protein/L, respectively, and 0.6 and 9.9 g fatty acids/L, respectively. Essential amino acid (EAA) in marination brines accounted for up to 59% of total amino acid (TAA). From mussel processing, cooking juice had more protein (14-23 g/L) than the rest of the process waters, and in all water types, EAA reached up to 42% of TAA. For all process waters, the most abundant nutritional elements were Na, K, P, Ca, and Se. The content of all potentially toxic elements was mostly below LOD, except for As which ranged from 0.07 to 1.07 mg/kg among all tested waters. Our findings shed light on liquid seafood side streams as untapped resources of nutrients which can be valorized into food/feed products.
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| 7. |
- Forghani Targhi, Bita, 1973, et al.
(författare)
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Protein and Long-Chain n-3 Polyunsaturated Fatty Acids Recovered from Herring Brines upon Flocculation and Flotation─A Case Study
- 2023
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Ingår i: ACS Sustainable Chemistry and Engineering. - : American Chemical Society. - 2168-0485. ; 11:17, s. 6523-
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Tidskriftsartikel (refereegranskat)abstract
- A novel integrated process for recovery of protein-enriched biomasses from 5% presalting brines and spice brines of herring (Clupea harengus) was investigated by combining carrageenan- and/or acid-driven flocculation (F) plus dissolved air flotation (DAF). The F-DAF technique with carrageenan resulted in protein and lipid recoveries from 5% presalting brine of 78 and 38%, respectively. Without flocculation or with only acidification, protein and lipid recoveries in DAF were only 13 and 10%, respectively. Low protein and lipid recoveries, 8-12 and 1.8-8.2%, respectively, were also obtained when spice brine was subjected to only acidification and DAF. The protein content in dry biomasses from 5% presalting brine and spice brine was 36-43 and 13-16%, respectively. The corresponding lipid levels were 23-31 and 9-18%, respectively, with ash levels of 11-20 and 38-45%, respectively. Biomass proteins contained ≤45% essential amino acids, and the lipids had ≤16% long-chain n-3 polyunsaturated fatty acids. Freeze-dried spice brine biomasses were characterized by anchovy- and spice-related sensory attributes. 5% presalting brine biomasses were connected to fish and seafood attributes and showed gel forming capacity. The outlined F-DAF recovery system can thus recover both nutrients and interesting flavors from the herring process waters, which are currently lost from the food chain. © 2023 The Authors.
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| 8. |
- Forghani Targhi, Bita, 1973, et al.
(författare)
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Recovery of a protein-rich biomass from shrimp (Pandalus borealis) boiling water: A colloidal study
- 2020
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Ingår i: Food Chemistry. - : Elsevier BV. - 0308-8146 .- 1873-7072. ; 302, s. 125299-
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Tidskriftsartikel (refereegranskat)abstract
- Flocculation and sedimentation of a protein-rich biomass from shrimp boiling water (SBW) using food grade polysaccharides (carrageenan, alginate and carboxymethyl cellulose (CMC)) as flocculants was investigated at different pH-values. The effect of flocculant concentration on particle size and viscosity of SBW was also evaluated. Flocculation with carrageenan (0.45 g/L) at pH = 4 exhibited the most efficient protein sedimentation; protein concentration of the upper phase was here reduced by 77%, allowing 86% protein to be sedimented from SBW. Flocculation by alginate and CMC at pH = 4 showed 67% and 60% protein reduction of the upper phase at concentrations of 0.5 and 0.2 g/L, respectively. Contrary to alginate and CMC, carrageenan concentration affected the size distribution of flocs. Finally, carrageenan at 0.45 g/L and pH = 4 was successfully tested in a scaled up trial (5L) providing 78.5% protein recovery and a biomass with 75% protein on dry weight basis.
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| 9. |
- Gültekin Subasi, Büsra, 1986, et al.
(författare)
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Exploring Swedish pea varieties suitable for protein isolation, focusing on antinutrients and off-flavors
- 2024
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Ingår i: Journal of Food Composition and Analysis. - 0889-1575 .- 1096-0481. ; 128
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Tidskriftsartikel (refereegranskat)abstract
- Six different pea (Pisum sativum) varieties and their proteins isolated via wet fractionation were screened to find varieties with outstanding protein functionality but minimum contents of antinutrients and off-flavor volatiles. A broad difference in emulsion activity (44.7–74.2 m2/g) and foaming capacity (163–210%) were detected between the varieties. Pea variety significantly affected LOX activity of the sample, yielding outstanding decreases (1.6–28.6 times) for all varieties following protein isolation. Variety Eso had the highest hexanal increase ratio (820 times) while variety Balder had the lowest (32 times) after the protein isolation. The total concentration of volatile off-flavors, phytate, and saponin increased during the protein isolation with distinctive degrees for each variety. The content of the antinutrients in the proteins was substantially affected by the variety. Altogether, purpose-specific selection of pea varieties based on their desired potential could enable pea proteins with fewer antinutrients and off-flavors.
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| 10. |
- Hedén, Ida, 1984, et al.
(författare)
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Dietary replacement of fishmeal with marine proteins recovered from shrimp and herring process waters promising in Atlantic salmon aquaculture
- 2023
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Ingår i: Aquaculture. - 0044-8486. ; 574
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Tidskriftsartikel (refereegranskat)abstract
- There is a general agreement that fish meal (FM) and fish oil (FO) are valuable resources for aquafeed, but that the production cannot keep the same pace as the current growth of the aquaculture industry. Therefore, there is a need to find alternative sources for lipids and protein. This study examines the possibility of using proteins recovered from seafood industry side stream waters as a complement to FM in feed for Atlantic salmon. To recover the proteins, herring and shrimp process side streams waters were flocculated then treated with dissolved air flotation (DAF), which is considered a gentle technology. Shrimp steam water was used to obtain shrimp protein (SP) and herring salt brine to obtain herring protein (HP). The recovered semi-solid protein fraction was spray dried and formulated into experimental diets at inclusion levels of 1.9–10% (dw/dw). Two feeding trials were conducted to investigate; 1) the properties of different flocculants to recover SP from shrimp steam water, alginate (Alg; 1.9% inclusion), carrageenan (Carr; 2.0% inclusion) and a synthetic flocculant from Kemira (Kem; 3.8% inclusion). 2) total or partial replacement of FM with SP-Alg (10% and no FM) and HP-Alg (3% and 8% FM). For both feeding trials each diet was provided to triplicate tanks (n = 31 and 30/tank) of Atlantic salmon with a start weight of 193 g (duration 10 weeks) and 304 g (duration 7 weeks) respectively. All fish showed similar feed intake, feed conversion ratio (on tank basis) and weight gain. The inclusion of SP or HP as total or partial replacement of FM did neither influence adiposity of the fish, as measured by condition factor, nor heposomatic index (HSI). No negative effect of the alternative protein could be found through histological examination of the intestine. In feeding trial two, diets did not affect the adaptive immune indicators CD8α and MHC II. The SP-Alg diet did not affect intestinal barrier and transporting functions, assessed using Ussing-chamber technology. However, HP-Alg affected the trans-epithelial resistance, which indicate that the intestinal barrier function could be affected by low inclusions. We conclude that from a biological perspective, SP recovered from shrimp steaming waters using Alg and DAF technology represents a new marine biomass with potential as a replacement for FM in Atlantic salmon feed. To diversify the possibility of using flocculants to retrieve proteins we also suggest further investigation of the potential to use Carr in larger inclusions.
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