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- Kristiansson, A., et al.
(författare)
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Correction to: Protection of Kidney Function with Human Antioxidation Protein α1-Microglobulin in a Mouse 177Lu-DOTATATE Radiation Therapy Model by Kristiansson A, Ahlstedt J, Holmqvist B, Brinte A, Tran TA, Forssell-Aronsson E, Strand S-E, Gram M, and Åkerström B. Antioxidants & Redox Signaling 30: 1746-1759, 2019. DOI: 10.1089/ars.2018.7517.
- 2020
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Ingår i: Antioxidants & redox signaling. - : Mary Ann Liebert Inc. - 1557-7716 .- 1523-0864. ; 32:9
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Tidskriftsartikel (refereegranskat)
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| 2. |
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| 3. |
- Carlsson, J, et al.
(författare)
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Therapy with radiopharmaceuticals
- 2002
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Ingår i: Acta Oncologica. - 1651-226X. ; 41:7-8, s. 623-628
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Tidskriftsartikel (refereegranskat)abstract
- In an investigation by the Swedish Cancer Society, an expert group described the present status, critical issues and future aspects and potentials for each of nine major areas of radiation therapy research. The present report deals with therapy with radiopharmaceuticals.
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| 4. |
- Dello, Nishte, et al.
(författare)
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Effect of Antioxidant rA1M on Expression of Apoptosis and Oxidative-Stress-Related Genes during 177Lu-Octreotate Treatment of GOT1 Neuroendocrine Tumours
- 2021
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Ingår i: 5th European Radiation Protection Week. Book of Abstracts. Vienna, Austria, 2021, 22-25 November, p. 58.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Kidney radiotoxicity during targeted radionuclide therapy with 177Lu-octreotate in patients with neuroendocrine tumours (NETs) limits the administered activity and thereby the therapeutic response. Reducing renal side effects of radiation could allow for higher activity amounts and better tumour control, increasing the cure rate. Co-treatment with the human recombinant antioxidant α1– microglobulin (rA1M) protects against short and long-termed radiation-induced renal damage in mice treated with 177Lu-octreotate. Furthermore, co-treatment with rA1M does not affect the therapeutic effects on tumour volume in human NET GOT1-bearing mice. Purpose: The aim of this work was to investigate transcriptional regulation of genes related to apoptosis and oxidative stress in GOT1 tumours in mice at one and seven days after administration of rA1M and after treatment with 177Lu-octreotate with or without co-administration with rA1M. Methods: Adult female Balb/c mice with s.c. GOT1 tumours were i.v. injected with either 177Luoctreotate (30 MBq, n=6), rA1M (5mg/kg, n=6) or co-treatment with both (n=6). A control group was sham-treated with NaCl (n=4). RNA was extracted from tumours sampled at one or seven days after injection. Gene expression of 84 apoptosis and 84 oxidative stress-related genes were evaluated using RNA array plates and RT-qPCR. Results: RT-qPCR analysis showed higher and similar expression of apoptotic genes in the irradiated groups than rA1M administration alone on day one after injection. Corresponding result after seven days was similar but less prominent. The most affected pro-and anti-apoptotic genes were GADD45A, TNFRS10B, FADD, FAS, CASP4,6,3, BAX and BAG3, BIRC2, BIRC3, BIRC5, XIAP, NAIP, BCL2L10, BCL2L2, IGF-1R, IL-10, CFLAR, respectively. Overall, most oxidative stress genes were not significantly regulated. APOE, DUOX1, HMOX and FOXM1 were some of the most regulated genes in the oxidative stress array. The results were more similar between the irradiated groups than those in the A1M group. CYBG and MB, related to oxygen transportation, were significantly upregulated after 177Lu-octreotate and coadministration, but not after rA1M alone. Conclusions: The transcriptional regulation of the analyzed apoptosis-related genes may suggest a similar apoptotic response in irradiated and co-treated groups compared to administration of rA1M alone. Antioxidant genes were generally downregulated, indicating that antioxidant defense mechanisms were less prioritized in damaged cells.
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| 8. |
- Kölby, Lars, 1963, et al.
(författare)
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A transplantable human carcinoid as model for somatostatin receptor-mediated and amine transporter-mediated radionuclide uptake.
- 2001
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Ingår i: The American journal of pathology. - 0002-9440. ; 158:2, s. 745-55
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Tidskriftsartikel (refereegranskat)abstract
- A human midgut carcinoid tumor was successfully transplanted into nude mice and propagated for five consecutive generations (30 months) with well-preserved phenotype. Tumor cells in nude mice expressed identical neuroendocrine markers as the original tumor, including somatostatin receptors (somatostatin receptors 1 to 5) and vesicular monoamine transporters (VMAT1 and VMAT2). Because of the expression of somatostatin receptors and VMAT1 and VMAT2 the grafted tumors could be visualized scintigraphically using the somatostatin analogue 111In-octreotide and the catecholamine analogue 123I-metaiodobenzylguanidine. The biokinetics of the somatostatin analogue 111In-octreotide in the tumors was studied and showed a high retention 7 days after administration. Cell cultures were re-established from transplanted tumors. Immunocytochemical and ultrastructural studies confirmed the neuroendocrine differentiation. The human origin of transplanted tumor cells was confirmed by cytogenetic and fluorescence it situ hybridization analyses. Spontaneous secretion of serotonin and its metabolite, 5-hydroxyindole acetic acid, from tumor cells was demonstrated. The tumor cells increased their [Ca2+]i in response to beta-adrenoceptor stimulation (isoproterenol) and K+-depolarization. All somatostatin receptor subtypes could be demonstrated in cultured cells. This human transplantable carcinoid tumor, designated GOT1, grafted to nude mice, will give unique possibilities for studies of somatostatin receptor- and VMAT-mediated radionuclide uptake as well as for studies of secretory mechanisms.
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| 9. |
- Lagerstrand, Kerstin M, et al.
(författare)
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Flow-induced disturbances in balanced steady-state free precession images: means to reduce or exploit them.
- 2009
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Ingår i: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine. - : Wiley. - 1522-2594. ; 61:4, s. 893-8
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Tidskriftsartikel (refereegranskat)abstract
- In this work computer simulations and phantom measurements are presented that show the effect of flow on in-plane balanced steady-state free precession images. The images were studied for various flow velocities, excitation regions, relaxation times, RF-pulse angles, and off-resonance frequencies. The work shows that flow-induced disturbances are present in the images, but can be reduced by the application of inhomogeneous excitation regions. Also, a velocity quantification method that utilizes the disturbances was developed and proved to quantify flow velocities accurately. The work concluded that the flow-induced disturbances can be reduced to improve image quality, but can also be exploited to quantify the flow velocity.
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