| 1. |
|
|
| 2. |
- Cajuso, T, et al.
(författare)
-
Retrotransposon insertions can initiate colorectal cancer and are associated with poor survival
- 2019
-
Ingår i: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 10:1, s. 4022-
-
Tidskriftsartikel (refereegranskat)abstract
- Genomic instability pathways in colorectal cancer (CRC) have been extensively studied, but the role of retrotransposition in colorectal carcinogenesis remains poorly understood. Although retrotransposons are usually repressed, they become active in several human cancers, in particular those of the gastrointestinal tract. Here we characterize retrotransposon insertions in 202 colorectal tumor whole genomes and investigate their associations with molecular and clinical characteristics. We find highly variable retrotransposon activity among tumors and identify recurrent insertions in 15 known cancer genes. In approximately 1% of the cases we identify insertions in APC, likely to be tumor-initiating events. Insertions are positively associated with the CpG island methylator phenotype and the genomic fraction of allelic imbalance. Clinically, high number of insertions is independently associated with poor disease-specific survival.
|
|
| 3. |
|
|
| 4. |
- Petkevicius, K., et al.
(författare)
-
TLCD1 and TLCD2 regulate cellular phosphatidylethanolamine composition and promote the progression of non-alcoholic steatohepatitis
- 2022
-
Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 13:1
-
Tidskriftsartikel (refereegranskat)abstract
- The regulation of cellular phosphatidylethanolamine (PE) acyl chain composition is poorly understood. Here, the authors show that TLCD1 and TLCD2 proteins mediate the formation of monounsaturated fatty acid-containing PE species and promote the progression of non-alcoholic steatohepatitis. The fatty acid composition of phosphatidylethanolamine (PE) determines cellular metabolism, oxidative stress, and inflammation. However, our understanding of how cells regulate PE composition is limited. Here, we identify a genetic locus on mouse chromosome 11, containing two poorly characterized genes Tlcd1 and Tlcd2, that strongly influences PE composition. We generated Tlcd1/2 double-knockout (DKO) mice and found that they have reduced levels of hepatic monounsaturated fatty acid (MUFA)-containing PE species. Mechanistically, TLCD1/2 proteins act cell intrinsically to promote the incorporation of MUFAs into PEs. Furthermore, TLCD1/2 interact with the mitochondria in an evolutionarily conserved manner and regulate mitochondrial PE composition. Lastly, we demonstrate the biological relevance of our findings in dietary models of metabolic disease, where Tlcd1/2 DKO mice display attenuated development of non-alcoholic steatohepatitis compared to controls. Overall, we identify TLCD1/2 proteins as key regulators of cellular PE composition, with our findings having broad implications in understanding and treating disease.
|
|
| 5. |
- Forsstrom, J., et al.
(författare)
-
Influence of different storage conditions on deinking efficiency of waterbased flexographic ink from model cellulose surfaces and sheets
- 2004
-
Ingår i: Nordic Pulp & Paper Research Journal. - : Walter de Gruyter GmbH. - 0283-2631 .- 2000-0669. ; 19:2, s. 250-256
-
Tidskriftsartikel (refereegranskat)abstract
- The influence of UV-Iight, temperature, atmospheric environment and storage time on the ink detachment of water-based flexographic ink printed on model cellulose surfaces was investigated using an impinging jet cell equipment. The printed surfaces were deinked using a NaOH solution (pH = 10) and the deinking process was monitored using a microscope equipped with a CCD camera. Images were collected at different time intervals during the detachment process and image analysis was used to quantify the ink detachment from the surface. Hand sheets, (the same pulp as used for model surface preparation) were also printed and stored under the same conditions, after which they were reslushed and deinked. The deinking efficiency of the recycled sheets was evaluated using brightness and ERIC (Effective Residual Ink Concentration) values. It was shown that UV-Iight had a negative effect on ink detachment both from the model cellulose surfaces and from the hand sheets. At storage temperatures of 55degreesC, (dark conditions were used) a large negative effect was observed for the cellulose surfaces while only a small effect on the ink detachment could be seen for the hand sheets. Ink detachment from the hand sheets became more difficult when increasing the storage temperature above 55degreesC, as detected as a decrease in brightness of the recycled and deinked sheets. A farther increase in the storage temperature to 105degreesC gave poorer ink detachment efficiency than storage under UV-Iight for the hand sheets. Air had a more negative effect on ink detachment than nitrogen.
|
|
| 6. |
- Forsstrom, J., et al.
(författare)
-
Influence of pore structure and water retaining ability of fibres on the strength of papers from unbleached kraft fibres
- 2005
-
Ingår i: Nordic Pulp & Paper Research Journal. - : Walter de Gruyter GmbH. - 0283-2631 .- 2000-0669. ; 20:2, s. 176-185
-
Tidskriftsartikel (refereegranskat)abstract
- The influence of pore structure and the water retaining ability of fibres on different strength properties of papers from unbleached kraft fibres was investigated and the effects of pulp yield, counter-ion types, pH and homification were determined. NMR relaxation measurements of water were used to determine the pore structure of the fibres and WRV and FSP measurements were used to determine their water retaining ability. The average pore radius, as determined by NMR, was almost unaffected by changes in pulp yield whereas changes in counter-ion and pH had a significant effect on the average pore radius. The detected changes in NMR were suggested to be due to changes in the swelling forces both within the fibre wall and at the fibre surface. The WRV value decreased with decreasing yield and it was significantly affected by pH and counter-ion. Changes in WRV were explained to be largely associated with changes in the amount of water associated with the fibre surface. The FSP values decreased with decreasing yield just as the WRV's. Homification upon drying and reslushing significantly lowered the average pore radius, whereas the FSP only showed a minor decrease, suggesting that the surface area available to water was changed without drastically changing the overall fibre wall volume. The differences between FSP NMR and WRV can hence be traced back to what the methods are actually measuring. It was concluded that the different measuring methods contain unique information and that a combination of the methods is necessary to give as complete a picture as possible over the changes that occur in the fibre wall upon varying the condition for the fibres. The influence of pore size on sheet tensile properties was also investigated. It was found that fibres with larger pores produced an increased tensile index and tensile stiffness of the paper made from these fibres. It was suggested that fibres with larger pores allow for a larger molecular contact area between fibres, stronger fibre/fibre joints and consequently a higher strength of the formed sheets.
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
|
|
