| 1. |
- Sartelli, Massimo, et al.
(författare)
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Ten golden rules for optimal antibiotic use in hospital settings: the WARNING call to action
- 2023
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Ingår i: WORLD JOURNAL OF EMERGENCY SURGERY. - 1749-7922. ; 18:1
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Forskningsöversikt (refereegranskat)abstract
- Antibiotics are recognized widely for their benefits when used appropriately. However, they are often used inappropriately despite the importance of responsible use within good clinical practice. Effective antibiotic treatment is an essential component of universal healthcare, and it is a global responsibility to ensure appropriate use. Currently, pharmaceutical companies have little incentive to develop new antibiotics due to scientific, regulatory, and financial barriers, further emphasizing the importance of appropriate antibiotic use. To address this issue, the Global Alliance for Infections in Surgery established an international multidisciplinary task force of 295 experts from 115 countries with different backgrounds. The task force developed a position statement called WARNING (Worldwide Antimicrobial Resistance National/International Network Group) aimed at raising awareness of antimicrobial resistance and improving antibiotic prescribing practices worldwide. The statement outlined is 10 axioms, or "golden rules," for the appropriate use of antibiotics that all healthcare workers should consistently adhere in clinical practice.
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| 2. |
- Garcia-Alonso, Luz, et al.
(författare)
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Mapping the temporal and spatial dynamics of the human endometrium in vivo and in vitro
- 2021
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Ingår i: Nature Genetics. - : Springer Nature. - 1061-4036 .- 1546-1718. ; 53:12, s. 1698-
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Tidskriftsartikel (refereegranskat)abstract
- The endometrium, the mucosal lining of the uterus, undergoes dynamic changes throughout the menstrual cycle in response to ovarian hormones. We have generated dense single-cell and spatial reference maps of the human uterus and three-dimensional endometrial organoid cultures. We dissect the signaling pathways that determine cell fate of the epithelial lineages in the lumenal and glandular microenvironments. Our benchmark of the endometrial organoids reveals the pathways and cell states regulating differentiation of the secretory and ciliated lineages both in vivo and in vitro. In vitro downregulation of WNT or NOTCH pathways increases the differentiation efficiency along the secretory and ciliated lineages, respectively. We utilize our cellular maps to deconvolute bulk data from endometrial cancers and endometriotic lesions, illuminating the cell types dominating in each of these disorders. These mechanistic insights provide a platform for future development of treatments for common conditions including endometriosis and endometrial carcinoma. Single-cell and spatial transcriptomic profiling of the human endometrium highlights pathways governing the proliferative and secretory phases of the menstrual cycle. Analyses of endometrial organoids show that WNT and NOTCH signaling modulate differentiation into the secretory and ciliated epithelial lineages, respectively.
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| 3. |
- Karlsson, Freddie, et al.
(författare)
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Dynamic response of driven end-bearing piles and a pile group in soft clay : an experimental validation study
- 2022
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Ingår i: Engineering structures. - : Elsevier BV. - 0141-0296 .- 1873-7323. ; 267
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents novel measurement data on the dynamic soil-structure interaction of an end-bearing pile foundation. The purpose is to assess the ability to predict the foundation impedances based on the small-strain properties of the soil obtained from site investigations. Measurements were performed in two stages of construction, allowing to assess interaction between the piles through the soil. First, single pile impedances and interaction factors between the piles were experimentally obtained for the four piles when they were free to move at the surface. Second, the impedances of the square pile group were measured after casting a concrete pile cap. The piles were additionally instrumented with accelerometers at depth along the centerline of each pile, allowing to illustrate the global behaviour of the piles within the soil. Numerical predictions based solely on information of the small-strain soil properties obtained from extensive site investigations are compared to the experimental results. The impedances of the individual piles are overestimated compared to the measurements, while the interaction factors show a better agreement. The pile group impedances are better captured than the individual ones, using the same soil model. The pile-soil-pile interaction is clearly manifested in the experimental results by pronounced peaks in the pile group vertical impedance, validating results from previous numerical studies.
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| 4. |
- Theland, Freddie, et al.
(författare)
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Assessment of small-strain characteristics for vibration predictions in a Swedish clay deposit
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Environmental vibrations induced by human activities such as traffic, construction or industrial manufacturing can cause disturbance among residents or to vibration sensitive equipment inbuildings. In Sweden, geological formations of soft clay overlying a stiff bedrock are soil conditions prone to ground vibrations that are encountered both in urban areas and along parts of the national railway network. This paper presents an extensive investigation of the small-strain soil properties for the prediction of environmental ground vibrations in a shallow clay where the bedrock is situated at 7.5 m depth. The small-strain properties are estimated using available empirical correlations, bender elements, seismic cone penetration tests, seismic refraction and inversion of surface wave dispersion and attenuation curves. The results are synthesised into a dynamic layered soil model which is validated by measurements at the soil’s surface at source-receiver distances up to 90 m in the frequency range 1–80 Hz. Analyses of uncertainties in the estimated values of wave speeds and material damping are performed by model investigations, indicating that surface wave tests overestimate the damping compared to bender element tests. The properties of the topmost unsaturated part of the soil is found to have a significant influenceon the response at large distances, caused by critically refracted P-waves resonating in the top layer.
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| 5. |
- Theland, Freddie, et al.
(författare)
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Assessment of small-strain characteristics for vibration predictions in a Swedish clay deposit
- 2021
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Ingår i: Soil Dynamics and Earthquake Engineering. - : Elsevier BV. - 0267-7261 .- 1879-341X. ; 150
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Tidskriftsartikel (refereegranskat)abstract
- Environmental vibrations induced by human activities such as traffic, construction or industrial manufacturing can cause disturbance among residents or to vibration sensitive equipment in buildings. In Sweden, geological formations of soft clay overlying a stiff bedrock are soil conditions prone to ground vibrations that are encountered both in urban areas and along parts of the national railway network. This paper presents an extensive investigation of the small-strain soil properties for the prediction of environmental ground vibrations in a shallow clay where the bedrock is situated at 7.5 m depth. The small-strain properties are estimated using available empirical correlations, bender elements, seismic cone penetration tests, seismic refraction and inversion of surface wave dispersion and attenuation curves. The results are synthesised into a dynamic layered soil model which is validated by measurements at the soil's surface at source-receiver distances up to 90 m in the frequency range 1-80 Hz. Analyses of uncertainties in the estimated values of wave speeds and material damping are performed by model investigations, indicating that surface wave tests overestimate the damping compared to bender element tests. The properties of the topmost unsaturated part of the soil is found to have a significant influence on the response at large distances, caused by critically refracted P-waves resonating in the top layer.
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| 6. |
- Theland, Freddie, 1989-, et al.
(författare)
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Dynamic response of driven end-bearing piles and a pile group in soft clay: an experimental validation study
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- This paper presents an extensive measurement campaign of the dynamic response of driven end-bearing piles and a pile group in soft clay. Results from measurements conducted on the free-top piles and and the piles joined in a concrete cap are presented. The measured frequency responses are subsequently used to obtain the impedances, characterising the dynamic stiffness and damping of the piles and the pile foundation. The piles are instrumented with accelerometers at several points along the length of the piles which allows to validate the dynamic motion of the piles atdepth due to pile head and pile cap excitation. Numerical predictions based solely on information of the small-strain soil properties obtained from extensive site investigations are compared to the experimental results. The numerical model captures the pile-soil-pile interactions and the motion of the piles within the soil well, suggesting that reliable predictions can be made for the considered soil conditions if the small-strain soil properties are well known.
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| 7. |
- Vanek, Daniel, et al.
(författare)
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Results of a collaborative study on DNA identification of aged bone samples
- 2017
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Ingår i: Croatian Medical Journal. - : MEDICINSKA NAKLADA. - 0353-9504 .- 1332-8166. ; 58:3, s. 203-213
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Tidskriftsartikel (refereegranskat)abstract
- Aim: A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples.Methods: Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory.Results: Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality.Conclusion: The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.
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| 8. |
- Zangeneh, Abbas, 1980-, et al.
(författare)
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Modal analysis of coupled soil-structure systems
- 2021
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Ingår i: Soil Dynamics and Earthquake Engineering. - : Elsevier BV. - 0267-7261 .- 1879-341X. ; 144
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents an efficient approach for the modal analysis of coupled soil-structure systems, for which the dynamic response is strongly influenced by the embedment in the soil. The methodology is based on a finite element-perfectly matched layer model that allows for the derivation of frequency-independent system matrices and the computation of the modal properties of the coupled system. This is achieved by solving a nonlinear eigenproblem using a Compact Rational Krylov (CoRK) eigensolver. A procedure is developed to sort the computed eigenpairs, filter out the spurious modes of the system which are related to the near-field and truncated far-field soil subdomains and select the physical structural modes of system. The proposed method can be used in the dynamic assessment and structural identification of strongly coupled soil-structure systems such as fully or partially buried structures and allows for the interpretation of experimentally identified modal properties of these systems, especially in the presence of highly damped or closely spaced coupled modes. The applicability and the scalability of the proposed approach for 2D and 3D problems is demonstrated in two case studies.
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