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- Imanishi, T., et al.
(författare)
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Integrative annotation of 21,037 human genes validated by full-length cDNA clones
- 2004
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Ingår i: PLoS biology. - : Public Library of Science (PLoS). - 1544-9173 .- 1545-7885. ; 2:6, s. 856-875
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Tidskriftsartikel (refereegranskat)abstract
- The human genome sequence defines our inherent biological potential; the realization of the biology encoded therein requires knowledge of the function of each gene. Currently, our knowledge in this area is still limited. Several lines of investigation have been used to elucidate the structure and function of the genes in the human genome. Even so, gene prediction remains a difficult task, as the varieties of transcripts of a gene may vary to a great extent. We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level. Our international collaboration has validated 21,037 human gene candidates by analysis of high-quality full-length cDNA clones through curation using unified criteria. This led to the identification of 5,155 new gene candidates. It also manifested the most reliable way to control the quality of the cDNA clones. We have developed a human gene database, called the H-Invitational Database (H-InvDB; http://www.h-invitational.jp/). It provides the following: integrative annotation of human genes, description of gene structures, details of novel alternative splicing isoforms, non-protein-coding RNAs, functional domains, subcellular localizations, metabolic pathways, predictions of protein three-dimensional structure, mapping of known single nucleotide polymorphisms (SNPs), identification of polymorphic microsatellite repeats within human genes, and comparative results with mouse full-length cDNAs. The H-InvDB analysis has shown that up to 4% of the human genome sequence (National Center for Biotechnology Information build 34 assembly) may contain misassembled or missing regions. We found that 6.5% of the human gene candidates (1,377 loci) did not have a good protein-coding open reading frame, of which 296 loci are strong candidates for non-protein-coding RNA genes. In addition, among 72,027 uniquely mapped SNPs and insertions/deletions localized within human genes, 13,215 nonsynonymous SNPs, 315 nonsense SNPs, and 452 indels occurred in coding regions. Together with 25 polymorphic microsatellite repeats present in coding regions, they may alter protein structure, causing phenotypic effects or resulting in disease. The H-InvDB platform represents a substantial contribution to resources needed for the exploration of human biology and pathology.
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3. |
- Ideguchi, E., et al.
(författare)
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Study of high-spin states in 48Ca region induced by secondary fusion reactions
- 2005
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Konferensbidrag (refereegranskat)abstract
- A new method of in-beam gamma-ray spectroscopy induced by secondary fusion reactions, 37P+9Be and 46Ar+9Be is presented. Low-energy secondary beams of 37P and 46Ar ions of ∼5 MeV/A were developed in order to induce fusion evaporation reactions. Excited states of nuclei in the vicinity of 48Ca, 49-52Ti and 46Ca, were studied by the method as well as β decay of the secondary beam 46Ar. Gamma-gamma coincidence and excitation function analysis were performed to study high-spin states of Ti isotopes.
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4. |
- Ideguchi, E, et al.
(författare)
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Study of high-spin states in the Ca-48 region by using secondary fusion reactions
- 2005
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Ingår i: European Physical Journal A. - : Springer Science and Business Media LLC. - 1434-6001 .- 1434-601X. ; 25, s. 429-430
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Tidskriftsartikel (refereegranskat)abstract
- An in-beam gamma-ray spectroscopy study, following a fusion reaction induced by a neutronrich secondary beam, Ar-46 + Be-9, is presented. A low-energy secondary beam of Ar-46 at similar to 5 MeV/A was developed in order to induce fusion reactions. Gamma-gamma coincidence and excitation function analysis was performed to study high-spin states in the vicinity of Ca-48, Ti49-52.
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5. |
- Shimoura, S., et al.
(författare)
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Lifetime of the isomeric O-2(+) state in Be-12
- 2007
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Ingår i: Physics Letters. Section B: Nuclear, Elementary Particle and High-Energy Physics. - : Elsevier BV. - 0370-2693. ; 654:3-4, s. 87-91
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Tidskriftsartikel (refereegranskat)abstract
- Mean lifetime tau of the isomeric O-2(+) state in Be-12 has been determined by measuring decay spectra of delayed y-rays from stopped Be-12 2 nuclei produced by the projectile fragmentation of O-18 at 100 A MeV. A consistent value of tau = 331 +/- 12 ns was obtained from the time spectra of the E2 gamma decay to the 2(1)(+) state and the positron annihilation following the E0 decay to the ground state. Based on the observed branching ratio between the E2 and E0 decays, transition strengths of the two decay modes were deduced to be B(E2; O-2(+) -> 2(1)(+)) = 7.0 +/- 0.6 e(2) fm(4) and 2 1 1 (O-2(+) Sigma(i) e(i) r(i)(2) O-1(+)), = 0.87 +/- 0.03 e fM(2), respectively. (C) 2007 Elsevier B.V. All rights reserved.
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6. |
- Kishida, T., et al.
(författare)
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High-spin isomeric beam line
- 2002
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Ingår i: Nuclear Instruments and Methods in Physics Research Section A. - : Elsevier. - 0168-9002 .- 1872-9576. ; 484:03-jan, s. 45-55
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Tidskriftsartikel (refereegranskat)abstract
- A high-spin isomeric beam line has been constructed at RIKEN based on the inverse kinematics of fusion-evaporation reactions. The beam line provides high-spin isomers as secondary beams, whose intensity is more than 10(5) sec(-1). The characteristics and the present status of the beam line are described.
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7. |
- Gono, Y., et al.
(författare)
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Systematics of high-spin isomers in N=83 isotones and a high-spin isomer beam
- 2002
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Ingår i: European Physical Journal A. - 1434-6001 .- 1434-601X. ; 13:02-jan, s. 5-8
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Tidskriftsartikel (refereegranskat)abstract
- Isomers in N = 83 isotones of Z = 60 66 were studied systematically. Their spins and parities arc,49/2(+) and 27(+) for odd and odd-odd nuclei, respectively. Nearly constant excitation energies of these isomers indicated a decrease of a Z = 64 shell gap energy as Z decreases from 64 to 60 within the framework of a deformed independent-particle model (DIPM). Their configurations are [v(f(tau/2)h(9/2)i(13/2)), pi(h(11/2))(2)](49/2+) and [v(f(7/2)h(9/2)i(13/2)), pi(h(11/2))(2)(d(5/2))(-1)](27+) for odd and odd-odd nuclei, respectively. The shape of the yrast status changes suddenly at spin 49/2(odd) and 27(odd-odd) from a near spherical to an oblate shape. Transitions from isomers are highly hindered because of the shape changes. They may be categorized to be shape isomers. The development of a secondary beam produced by using these high-spin isomers is also described.
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8. |
- Watanabe, H., et al.
(författare)
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Application of the high-spin isomer beams to the secondary fusion reaction and the measurement of g-factor
- 2004
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Ingår i: Nuclear Physics A. - : Elsevier BV. - 0375-9474 .- 1873-1554. ; 746, s. 540C-543C
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Tidskriftsartikel (refereegranskat)abstract
- A technique for providing high-spin isomers as probes of the fusion reaction and the measurement of g-factor has been worked out at RIKEN. In the study of the fusion reaction C-12(Sm-145m,xn)Er157-x, the gamma rays emitted from the fusion-evaporation residue Er-154 have been successfully observed. The nuclear g-factor of the T-1/2 = 28 ns high-spin isomer in Dy-149 has been measured with the gamma-ray TDPAD method.
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9. |
- Xu, G., et al.
(författare)
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Functional analysis of platelet-derived growth factor receptor-β in neural stem/progenitor cells
- 2013
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Ingår i: Neuroscience. - : Elsevier BV. - 0306-4522. ; 238, s. 195-208
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Tidskriftsartikel (refereegranskat)abstract
- Activation of neural stem/progenitor cells (NSPCs) is a potential therapeutic strategy of neurological disorders. In this study, NSPCs of subventricular zone were isolated and cultured from platelet-derived growth factor-β-receptor-knockout (PDGFR-β−/−) mice of postnatal day 1 (P1) and P28, and the roles of PDGFR-β were examined in these cells. In PDGFR-β-preserving control NSPCs, stem cell activities, such as numbers and diameters of secondary neurospheres, cell proliferation and survival rates, were significantly higher in P1 NSPCs than those in P28 NSPCs. In PDGFR-β−/− NSPCs, most of these parameters were decreased as compared with age-matched controls. Among them, the decrease of secondary neurosphere formation was most striking in P1 and P28 PDGFR-β−/− NSPCs and in P28 control NSPCs as compared with P1 control NSPCs. PCR-array and following quantitative real-time PCR (qRT-PCR) analyses demonstrated that expressions of fibroblast growth factor-2 (FGF2) and exons IV–IX of brain-derived neurotrophic factor (BDNF) were decreased, and noggin was increased in P1 PDGFR-β−/− as compared with P1 controls. Addition of BDNF rescued the number and diameter of secondary neurospheres in P1 PDGFR-β−/− NSPCs to similar levels as controls. The expressions of PDGFs and PDGFRs in control NSPCs were increased along with the differentiation-induction, where phosphorylated PDGFR-β was co-localized with neuronal and astrocyte differentiation markers. In controls, the neuronal differentiation was decreased, and the glial differentiation was increased from P1 to P28 NSPCs. Compared with P1 controls, neuronal differentiation was reduced in P1 PDGFR-β−/− NSPCs, whereas glial differentiation was comparable between the two genotypes. These results suggest that PDGFR-β signaling is important for the self-renewal and multipotency of NSPCs, particularly in neonatal NSPCs. BDNF, FGF2, and noggin may be involved in the effects of PDGFR-β signaling in these cells. Accordingly, the activation of PDGFR-β in NSPCs may be a novel therapeutic strategy of neurological diseases.
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