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| 2. |
- Funk, C, et al.
(författare)
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Increased Secondary Product Formation in Plant Cell Suspension Cultures after Treatment with a Yeast Carbohydrate (Elicitor)
- 1987
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Ingår i: Phytochemistry. - 0031-9422 .- 1873-3700. ; 26, s. 401-405
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Tidskriftsartikel (refereegranskat)abstract
- A carbohydrate fraction isolated from yeast extract by ethanolic precipitation was used as an elicitor to induce secondary product formation in plant cell suspension cultures. The elicitor preparation is effective in inducing glyceollin isomer synthesis (up to 200 μg glyceollin per g dry wt) in cells of Glycine max and enhancing berberine biosynthesis (up to four-fold) in cells of Thalictrum rugosum. The response of the cell cultures to the elicitor treatment is dependent on the amount of carbohydrate per unit of biomass and on the physiological state of the cells. Cells are optimally induced in late exponential or early stationary growth phases.
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| 3. |
- Gügler, K, et al.
(författare)
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Elicitor-induced Tyrosine Decarboxylase in Berberine Synthesizing Suspension Cultures of Thalictrum rugosum
- 1988
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Ingår i: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 170:3, s. 661-666
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Tidskriftsartikel (refereegranskat)abstract
- Tyrosine decarboxylase (EC 4.1.1.25) was induced in suspension cultures of Thalictrum rugosum by treatment with a yeast glucan elicitor. Maximum induction was observed at a carbohydrate concentration of 0.4 mg/g fresh weight of cells and maximum enzyme activity was reached 20 h after addition of elicitor. The enzyme was inducible in late exponential and early stationary growth phases. A good correlation between induced tyrosine decarboxylase activity and berberine biosynthesis has been established. It is suggested that tyrosine decarboxylase may be a key enzyme between primary and secondary metabolisms in the biosynthesis of norlaudanosoline-derived alkaloids.
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