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- Amada, Takako, et al.
(författare)
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Development of an immunochromatography strip test based on truncated nucleocapsid antigens of three representative hantaviruses
- 2014
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Ingår i: Virology Journal. - 1743-422X. ; 11, s. 87-
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Tidskriftsartikel (refereegranskat)abstract
- Background: Hantaviruses are causative agents of hemorrhagic fever with renal syndrome (HFRS) and nephropathia epidemica (NE) in the Old World and hantavirus pulmonary syndrome (HPS) in the New World. There is a need for time-saving diagnostic methods. In the present study, recombinant N antigens were used as antigens in an immunochromatography strip (ICG) test to detect specific IgG antibodies. Methods: The N-terminal 103 amino acids (aa) of Hantaan virus (HTNV), Puumala virus (PUUV) and Andes virus (ANDV) nucleocapsid (N) protein were expressed in E. coli as representative antigens of three groups (HFRS, NE and HPS-causing viruses) of hantavirus. Five different types of ICG test strips, one antigen line on one strip for each of the three selected hantaviruses (HTNV, PUUV and ANDV), three antigen lines on one strip and a mixed antigen line on one strip, were developed and sensitivities were compared. Results: A total of 87 convalescent-phase patient sera, including sera from 35 HFRS patients, 36 NE patients and 16 HPS patients, and 25 sera from healthy seronegative people as negative controls were used to evaluate the ICG test. Sensitivities of the three-line strip and mixed-line strip were similar to those of the single antigen strip (97.2 to 100%). On the other hand, all of the ICG test strips showed high specificities to healthy donors. Conclusion: These results indicated that the ICG test with the three representative antigens is an effective serodiagnostic tool for screening and typing of hantavirus infection in humans.
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- Søndergaard, M. L. J., et al.
(författare)
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Feminist Voices about Ecological Issues in HCI
- 2022
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Ingår i: Conference on Human Factors in Computing Systems - Proceedings. - New York, NY, USA : Association for Computing Machinery (ACM).
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Konferensbidrag (refereegranskat)abstract
- Even though issues such as climate change, pollution, and declining biodiversity impact us all, people with historically disenfranchised and socio-politically marginalized (HDSM) identities often bear the harsher brunt of ecological crises and suffer disproportionately. There is a need for listening to the voices of people with intersecting HDSM identities in relation to feminist engagements with ecological issues as applicable to HCI and IxD research and practice. Building upon and braiding together two thriving HCI discourses on feminism and environmental sustainability, we invite submissions from researchers, designers, educators, and activists interested in the intersections of feminist and ecological issues with a priority towards the well-being of people with HDSM identities. Converging feminist concerns on power, voice, and public discourse through this online workshop distributed across three time-zones, we hope to provide a forum for contemporary feminist voices as agents of change while engaging with ecological issues through an intersectional feminist orientation.
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