| 1. |
- Klionsky, Daniel J., et al.
(författare)
-
Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
-
Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
-
Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
|
|
| 2. |
|
|
| 3. |
- Dong, Haoxuan, et al.
(författare)
-
Flexible Eco-cruising Strategy for Connected and Automated Vehicles with Efficient Driving Lane Planning and Speed Optimization
- 2024
-
Ingår i: IEEE Transactions on Transportation Electrification. - 2332-7782. ; 10:1, s. 1530-1540
-
Tidskriftsartikel (refereegranskat)abstract
- Eco-cruising control of vehicles is a potential approach for improving vehicle energy efficiency and reducing travel time. However, many eco-cruising studies merely focused on vehicle longitudinal speed optimization but overlooked the lane change maneuvers, which may impair the benefits of eco-cruising when the vehicle encounters the slowly moving preceding vehicle. This study proposes a flexible eco-cruising strategy (FECS) with efficient driving lane planning and speed optimization capabilities simultaneously for connected and automated vehicles. The FECS is designed with a hierarchical control framework, where the first layer uses the Dijkstra algorithm to plan an efficient driving lane sequence considering the long-term effect of the preceding vehicles, then guides the second layer to optimize the vehicle’s speed for saving energy using trigonometric speed profile. The optimized driving trajectory is implemented in the third layer by regulating the speed and yaw angle for guaranteeing safe inter-vehicle distance when uncertainties are present. Finally, stochastic simulation with randomized traffic flows and typical case analysis based on real-world traffic data are conducted to demonstrate the performance of the FECS. The results manifest FECS’s capability of lowering driving costs in moderate-flow and free-flow traffic. However, we note that the benefits are less pronounced in congested-flow traffic.
|
|
| 4. |
- Hou, Ya Qing, et al.
(författare)
-
Research progress in CALPHAD assisted metal additive manufacturing
- 2024
-
Ingår i: China Foundry. - : Springer Nature. - 1672-6421. ; 21:4, s. 295-310
-
Forskningsöversikt (refereegranskat)abstract
- Metal additive manufacturing (MAM) technology has experienced rapid development in recent years. As both equipment and materials progress towards increased maturity and commercialization, material metallurgy technology based on high energy sources has become a key factor influencing the future development of MAM. The calculation of phase diagrams (CALPHAD) is an essential method and tool for constructing multi-component phase diagrams by employing experimental phase diagrams and Gibbs free energy models of simple systems. By combining with the element mobility data and non-equilibrium phase transition model, it has been widely used in the analysis of traditional metal materials. The development of CALPHAD application technology for MAM is focused on the compositional design of printable materials, the reduction of metallurgical imperfections, and the control of microstructural attributes. This endeavor carries considerable theoretical and practical significance. This paper summarizes the important achievements of CALPHAD in additive manufacturing (AM) technology in recent years, including material design, process parameter optimization, microstructure evolution simulation, and properties prediction. Finally, the limitations of applying CALPHAD technology to MAM technology are discussed, along with prospective research directions.
|
|
| 5. |
- Jin, Ying-Hui, et al.
(författare)
-
Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19 : An evidence-based clinical practice guideline (updated version)
- 2020
-
Ingår i: Military Medical Research. - : Springer Science and Business Media LLC. - 2054-9369. ; 7:1
-
Tidskriftsartikel (refereegranskat)abstract
- The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of a rapidly spreading illness, coronavirus disease 2019 (COVID-19), affecting more than seventeen million people around the world. Diagnosis and treatment guidelines for clinicians caring for patients are needed. In the early stage, we have issued "A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version)"; now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline. We formed a working group of clinical experts and methodologists. The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas: chemoprophylaxis, diagnosis, treatments, and discharge management. We searched the literature for direct evidence on the management of COVID-19, and assessed its certainty generated recommendations using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach. Recommendations were either strong or weak, or in the form of ungraded consensus-based statement. Finally, we issued 34 statements. Among them, 6 were strong recommendations for, 14 were weak recommendations for, 3 were weak recommendations against and 11 were ungraded consensus-based statement. They covered topics of chemoprophylaxis (including agents and Traditional Chinese Medicine (TCM) agents), diagnosis (including clinical manifestations, reverse transcription-polymerase chain reaction (RT-PCR), respiratory tract specimens, IgM and IgG antibody tests, chest computed tomography, chest x-ray, and CT features of asymptomatic infections), treatments (including lopinavir-ritonavir, umifenovir, favipiravir, interferon, remdesivir, combination of antiviral drugs, hydroxychloroquine/chloroquine, interleukin-6 inhibitors, interleukin-1 inhibitors, glucocorticoid, qingfei paidu decoction, lianhua qingwen granules/capsules, convalescent plasma, lung transplantation, invasive or noninvasive ventilation, and extracorporeal membrane oxygenation (ECMO)), and discharge management (including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge). We also created two figures of these recommendations for the implementation purpose. We hope these recommendations can help support healthcare workers caring for COVID-19 patients.
|
|
| 6. |
- Loth, Daan W, et al.
(författare)
-
Genome-wide association analysis identifies six new loci associated with forced vital capacity
- 2014
-
Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 46, s. 669-677
-
Tidskriftsartikel (refereegranskat)abstract
- Forced vital capacity (FVC), a spirometric measure of pulmonary function, reflects lung volume and is used to diagnose and monitor lung diseases. We performed genome-wide association study meta-analysis of FVC in 52,253 individuals from 26 studies and followed up the top associations in 32,917 additional individuals of European ancestry. We found six new regions associated at genome-wide significance (P < 5 × 10(-8)) with FVC in or near EFEMP1, BMP6, MIR129-2-HSD17B12, PRDM11, WWOX and KCNJ2. Two loci previously associated with spirometric measures (GSTCD and PTCH1) were related to FVC. Newly implicated regions were followed up in samples from African-American, Korean, Chinese and Hispanic individuals. We detected transcripts for all six newly implicated genes in human lung tissue. The new loci may inform mechanisms involved in lung development and the pathogenesis of restrictive lung disease.
|
|
| 7. |
- Nan, Jun-hu, et al.
(författare)
-
Air-core characteristics in a swirling tunnel flow
- 2022
-
Ingår i: Journal of Hydrodynamics. - : Springer Nature. - 1001-6058 .- 1000-4874 .- 1878-0342. ; 34:4, s. 634-646
-
Tidskriftsartikel (refereegranskat)abstract
- A cost-effective technique to dissipate the energy in hydropower systems is the formation of a swirling flow within a tunnel. In such flows, an air core with a significant cross section usually occurs. To reveal the air-core features in the horizontal tunnel of a high-head shaft spillway, laboratory tests, numerical modeling, and prototype observations are performed, to examine issues such as the formation of the air core, the interjacent air motion, the air-carrying capacity, and the scale effects. It is shown that the shape of the air core varies greatly in the axial and radial directions along the tunnel and that the center of the core deviates from the axis of the tunnel. The motion of the air within the core is caused by the combined action of the water entrainment on the inner surface of the swirling flow and the axial pressure difference in the air core. The aeration process can be divided into five processes with respect to the changes of the gate openings. A theoretical expression is established for the air-carrying capacity of the swirling flow. The vacuum degree is the similarity condition of the air-carrying capacity of the swirling flow between the model and prototype tests based on the Froude law of the similitude, and this similarity condition is verified by both the model and prototype results. This work provides a reference for the application of the swirling flows in horizontal hydropower tunnels.
|
|
| 8. |
- Qiu, Feng, et al.
(författare)
-
A new IQ detection method for LLRF
- 2012
-
Ingår i: Nuclear Instruments & Methods in Physics Research. Section A: Accelerators, Spectrometers, Detectors, and Associated Equipment. - : Elsevier BV. - 0167-5087 .- 0168-9002. ; 675, s. 139-143
-
Tidskriftsartikel (refereegranskat)abstract
- Digital LLRF technology has been widely used in new generation particle accelerators. IF quadrature sampling is a common method for amplitude and phase detection. Many strategies, which obey the same rule of f(sample) = (M/N)f(IF) (M/N is a rational number), have been proposed to reduce the effects of spectrum aliasing. However, we found that M/N does not need to be a rational number according to Shannon's theorem. Therefore, we propose a new IQ detection method in this paper. This method is based on a special IIR filter which is derived from an RLC circuit. The unique characteristic of the method is that the value of f(IF) is independent of the value of, f(sample). We have set up an experimental platform to verify our method. A 122.88 MHz sampling clock is used to sample a 3 MHz IF signal. The DOS and PI control techniques are used to realize the closed-loop control. Results show that the stability of the system is within +/- 0.05% (peak to peak) for the amplitude, and with +/- 0.03 degrees (peak to peak) for the phase in 5 h. (C) 2012 Elsevier B.V. All rights reserved.
|
|
| 9. |
- Qu, Muchao, et al.
(författare)
-
Electrical conductivity of anisotropic PMMA composite filaments with aligned carbon fibers - predicting the influence of measurement direction
- 2020
-
Ingår i: RSC Advances. - : ROYAL SOC CHEMISTRY. - 2046-2069. ; 10:7, s. 4156-4165
-
Tidskriftsartikel (refereegranskat)abstract
- In order to study the electrical conductivity of anisotropic PMMA/carbon fiber (CF) composites, cylindrical PMMA/CF filaments were extruded through a capillary rheometer, resulting in an induced CF orientation along the extrusion direction. The aspect ratios of the CFs in the filaments were accurately regulated using a two-step melt mixing process. By measuring the vertical and horizontal resistances of filaments where the outermost layer was successively peeled off, the anisotropic conductivities could be calculated. This was done using a novel analytical model where each cylindrical composite filament was defined as a structure consisting of three concentric cylinders with potentially different conductivities and CF orientations. The electrical conductivity increased with the degree of fiber orientation along the voltage direction and the effects of anisotropy and measurement direction were incorporated into the (isotropic) McLachlan equation. The required distance for electrical contact between the CFs was calculated to be 16 nm. Finite element (FEM) simulations were successfully utilized to confirm the data.
|
|
| 10. |
- Qu, Muchao, et al.
(författare)
-
Mechanical and electrical properties of carbon nanotube/epoxy/glass-fiber composites intended for nondestructive testing
- 2023
-
Ingår i: Polymers for Advanced Technologies. - : Wiley. - 1042-7147 .- 1099-1581. ; 34:8, s. 2554-2563
-
Tidskriftsartikel (refereegranskat)abstract
- In this study, ternary polymer composites sheets comprising glass fiber (GF) reinforced epoxy with various fractions of carbon nanotubes (CNT) were manufactured using hot-pressing technology. A multiscale morphology analysis was presented using scanning electron microscopy. The thermal behavior of the glass fiber reinforced polymer (GFRP) was investigated using thermogravimetric analysis, DSC, and DMA, which indicated an application temperature up to 71°C for the composites. Mechanical uniaxial stretching and three-points bending tests showed that the addition of 0.1–0.2 wt% CNT decreased the dissipated energy of the specimen by 50% and increased the Young's modulus by more than 100%. During all stretching and bending measurements, the relative change in electrical resistance (RCR) was recorded as function of strain, revealing a relationship between the electrical signal and the applied deformation of the GFRP. Finally, the standard equation for fitting RCR versus strain was optimized, reducing the number of fitting parameters from five to three. The electrical and mechanical properties of the CNT/GF/epoxy composites show that they are suitable sensoring materials for wind-turbine blades and other glass-fiber reinforced epoxy constructions, especially for nondestructive testing.
|
|
