| 1. |
- Garrison, Julie A., 1993-, et al.
(författare)
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Molecular diet analysis enables detection of diatom and cyanobacteria DNA in the gut of Macoma balthica
- 2022
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Ingår i: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 17:11
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Tidskriftsartikel (refereegranskat)abstract
- Detritivores are essential to nutrient cycling, but are often neglected in trophic networks, due to difficulties with determining their diet. DNA analysis of gut contents shows promise of trophic link discrimination, but many unknown factors limit its usefulness. For example, DNA can be rapidly broken down, especially by digestion processes, and DNA provides only a snapshot of the gut contents at a specific time. Few studies have been performed on the length of time that prey DNA can be detected in consumer guts, and none so far using benthic detritivores. Eutrophication, along with climate change, is altering the phytoplankton communities in aquatic ecosystems, on which benthic detritivores in aphotic soft sediments depend. Nutrient-poor cyanobacteria blooms are increasing in frequency, duration, and magnitude in many water bodies, while nutrient-rich diatom spring blooms are shrinking in duration and magnitude, creating potential changes in diet of benthic detritivores. We performed an experiment to identify the taxonomy and quantify the abundance of phytoplankton DNA fragments on bivalve gut contents, and how long these fragments can be detected after consumption in the Baltic Sea clam Macoma balthica. Two common species of phytoplankton (the cyanobacteria Nodularia spumigena or the diatom Skeletonema marinoi) were fed to M. balthica from two regions (from the northern and southern Stockholm archipelago). After removing the food source, M. balthica gut contents were sampled every 24 hours for seven days to determine the number of 23S rRNA phytoplankton DNA copies and when the phytoplankton DNA could no longer be detected by quantitative PCR. We found no differences in diatom 18S rRNA gene fragments of the clams by region, but the southern clams showed significantly more cyanobacteria 16S rRNA gene fragments in their guts than the northern clams. Interestingly, the cyanobacteria and diatom DNA fragments were still detectable by qPCR in the guts of M. balthica one week after removal from its food source. However, DNA metabarcoding of the 23S rRNA phytoplankton gene found in the clam guts showed that added food (i.e. N. spumigena and S. marinoi) did not make up a majority of the detected diet. Our results suggest that these detritivorous clams therefore do not react as quickly as previously thought to fresh organic matter inputs, with other phytoplankton than large diatoms and cyanobacteria constituting the majority of their diet. This experiment demonstrates the viability of using molecular methods to determine feeding of detritivores, but further studies investigating how prey DNA signals can change over time in benthic detritivores will be needed before this method can be widely applicable to both models of ecological functions and conservation policy.
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| 2. |
- Garrison, Julie A., 1993-, et al.
(författare)
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Temporal and spatial changes in benthic invertebrate trophic networks along a taxonomic richness gradient
- 2022
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Ingår i: Ecology and Evolution. - : Wiley. - 2045-7758. ; 12:6
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Tidskriftsartikel (refereegranskat)abstract
- Species interactions underlie most ecosystem functions and are important for understanding ecosystem changes. Representing one type of species interaction, trophic networks were constructed from biodiversity monitoring data and known trophic links to assess how ecosystems have changed over time. The Baltic Sea is subject to many anthropogenic pressures, and low species diversity makes it an ideal candidate for determining how pressures change food webs. In this study, we used benthic monitoring data for 20 years (1980–1989 and 2010–2019) from the Swedish coast of the Baltic Sea and Skagerrak to investigate changes in benthic invertebrate trophic interactions. We constructed food webs and calculated fundamental food web metrics evaluating network horizontal and vertical diversity, as well as stability that were compared over space and time. Our results show that the west coast of Sweden (Skagerrak) suffered a reduction in benthic invertebrate biodiversity by 32% between the 1980s and 2010s, and that the number of links, generality of predators, and vulnerability of prey have been significantly reduced. The other basins (Bothnian Sea, Baltic Proper, and Bornholm Basin) do not show any significant changes in species richness or consistent significant trends in any food web metrics investigated, demonstrating resilience at a lower species diversity. The decreased complexity of the Skagerrak food webs indicates vulnerability to further perturbations and pressures should be limited as much as possible to ensure continued ecosystem functions.
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| 3. |
- Garrison, Julie, 1993-, et al.
(författare)
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Amphipod Isotope Composition, Condition and Reproduction in Contrasting Sediments : A Reciprocal Transfer Experiment
- 2022
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Ingår i: Frontiers in Marine Science. - : Frontiers Media SA. - 2296-7745. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Eutrophication is a process that results in excessive phytoplankton blooms, which sink to the sediment and enrich the organic matter (OM). This alters the available resources to benthic organisms and may have consequences for feeding ecology and reproduction strategies of marine populations. While effects of eutrophication on biodiversity are well documented, the more subtle effects of OM on population dynamics and diet plasticity are understudied. We performed a reciprocal transfer experiment with the benthic bioindicator amphipod Monoporeia affinis from two stations in the Baltic Sea with differing sediment OM (low and high) creating four treatments (low control, low transferred, high transferred, and high control). We investigated sediment OM effects on: i) the dietary niche and organism body condition of two different life stages of M. affinis utilizing bulk stable isotopes δ13C and δ15N, and C:N ratio; and ii) M. affinis fecundity and embryo viability. There was no initial significant differences between the females from different stations in terms of δ13C, δ15N, C:N, fecundity or viable embryos. However, we found that moving females from high OM to low OM (where the low OM sediment has higher δ15N and lower δ13C) significantly depleted their 13C values, while amphipods in low OM sediment had always significantly enriched 15N regardless of female origin indicating feeding on the new sediment. Although end-of-experiment females had lower C:N than initial females, individuals in low OM sediment presented significantly higher C:N (indicating higher body condition) than those in high OM sediment. Conversely to adult amphipods, no effects of OM were seen for juveniles δ13C or δ15N, but their individual biomass was larger in high OM sediment treatments and high OM transferred to low OM sediment. Our results indicate that the low range of sediment OM tested here altered female amphipod δ13C, δ15N and C:N ratios, with those in low OM treatments having a better body condition, but those in high OM treatments had a greater reproductive success in terms of offspring biomass. Our findings suggest a tradeoff between female condition and reproduction and indicates that even relatively small levels of sediment organic enrichment will impact female condition. Our study provides valuable information useful to interpret the effects of OM on amphipod populations used as bioindicators for anthropogenic impacts.
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