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- Eriksson, Oskar, et al.
(författare)
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Neutrophil CD64 expression - comparison of two different flow cytometry protocols on EPICs MCL and the Leuko64 (TM) assay on a Celldyn Sapphire haematology analyser
- 2015
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Ingår i: Scandinavian Journal of Clinical and Laboratory Investigation. - : Informa UK Limited. - 0036-5513 .- 1502-7686. ; 75:5, s. 428-433
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Tidskriftsartikel (refereegranskat)abstract
- Objective. To evaluate the Trillium Diagnostics Leuko64 (TM) assay on Abbott Celldyn Sapphire haematology analyser compared to two flow cytometry protocols on Beckman Coulter EPICS MCL flow cytometer. Materials and methods. CD64 expression on neutrophils was determined by two flow cytometry protocols and by a commercial assay on an automatic haematology analyser. The inclusion of study subjects was based on elevated procalcitonin (PCT) values, identifying patients where a systemic infection was suspected. Healthy blood donors were used as a reference group. Results. Statistically significant correlations between the Trillium Diagnostics Leuko64 (TM) assay and the flow cytometry methods were found when measuring neutrophil CD64 expression. Conclusions. The good correlation between a reference method and an automated haematology analyser method for CD64 expression on neutrophils supports introduction of the latter assay for routine use as an independent biomarker of bacterial infection and inflammation.
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- Fadeel, Bengt, et al.
(författare)
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Kostmann disease and other forms of severe congenital neutropenia
- 2021
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Ingår i: Acta Paediatrica. - : John Wiley & Sons. - 0803-5253 .- 1651-2227. ; 110:11, s. 2912-2920
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Tidskriftsartikel (refereegranskat)abstract
- Congenital neutropenia with autosomal recessive inheritance was first described by the Swedish paediatrician Rolf Kostmann who coined the term ‘infantile genetic agranulocytosis’. The condition is now commonly referred to as Kostmann disease. These patients display a maturation arrest of the myelopoiesis in the bone marrow and reduced neutrophil numbers and suffer from recurrent, often life-threatening infections. The molecular mechanism underlying congenital neutropenia has been intensively investigated, and mutations in genes that impinge on programmed cell death have been identified. The present review provides an overview of these studies.
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- Garwicz, Daniel
(författare)
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Biosynthesis, Processing, and Sorting of Human Neutrophil Granule Proteins. Focus on Cathepsin G, Proteinase 3, and Azurocidin
- 2000
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The neutrophil granulocyte contains a number of multifunctional proteins stored in different subsets of granules. The azurophil granules, synthesized early during differentiation, harbour leukocyte elastase, cathepsin G, proteinase 3, and azurocidin; the members of the serprocidin family. In order to study the biosynthesis, processing, and intracellular sorting of the proteins, human cDNA encoding the latter three proteins, in their preproform, was stably transfected to two hematopoietic rodent cell lines (RBL-1 and 32Dcl3). To investigate the importance of distinct protein motifs, mutations were made in the cDNAs prior to transfection. Cathepsin G lacking functional glycosylation site was normally activated and sorted to granules. Similarly, cathepsin G, lacking both functional catalytic site and amino-terminal propeptide, was stably transfected to both cell lines. However, preterm activation of catalytically active cathepsin G lead to impaired cell survival. Proteinase 3 was expressed and stored in a form that allowed specific recognition by human cANCA-sera from patients with Wegener's granulomatosis. The amino-terminal heptapropeptide of azurocidin was shown to be removed in a stepwise manner involving at least two different enzymes. All serprocidins acquired high-mannose oligosaccharides that were converted into complex forms. Finally, the biosynthetic windows of several neutrophil granule proteins, including myeloperoxidase, lysozyme, cathepsin D, and all four serprocidins, were characterized in an experimental in vitro model for differentiation of human bone marrow progenitor cells into neutrophils.
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- Garwicz, Daniel, et al.
(författare)
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Early recognition of reverse pseudohyperkalemia in heparin plasma samples during leukemic hyperleukocytosis can prevent iatrogenic hypokalemia
- 2012
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Ingår i: Clinical Biochemistry. - : Elsevier BV. - 0009-9120 .- 1873-2933. ; 45:18, s. 1700-1702
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: To investigate the cause of apparent hyperkalemia in leukemic heparin plasma. Design and methods: Lithium heparin plasma and serum samples from a patient with chronic lymphocytic leukemia (CLL) with hyperleukocytosis were transported by either a pneumatic tube system or manual transport and analyzed either immediately or after 4 h. Results: Pneumatic tube transported samples resulted in higher plasma potassium levels than manually transported samples. Serum potassium was lower than plasma potassium, confirming the suspicion of "reverse" pseudohyperkalemia. Letting the pneumatic tube transported samples stand on the bench for 4 h before centrifugation surprisingly resulted in decreased or unchanged plasma potassium. Conclusions: The reverse pseudohyperkalemia in heparin plasma samples from a CLL patient was caused by pneumatic tube transport. Our results suggest extracellular leakage of potassium, followed by active transport of potassium into intact leukemic cells. This is the first Swedish case of reverse pseudohyperkalemia in a CLL patient, where clinical suspicion of false hyperkalemia and awareness of the phenomenon lead to a rapid laboratory diagnosis. The demonstration of reverse pseudohyperkalemia prevented potentially dangerous medical interventions, such as potassium lowering treatment.
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