| 1. |
- Ni, J, et al.
(författare)
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Cystatin F is a glycosylated human low molecular weight cysteine proteinase inhibitor
- 1998
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Ingår i: Journal of Biological Chemistry. - 1083-351X. ; 273:38, s. 24797-24804
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Tidskriftsartikel (refereegranskat)abstract
- A previously undescribed human member of the cystatin superfamily called cystatin F has been identified by expressed sequence tag sequencing in human cDNA libraries. A full-length cDNA clone was obtained from a library made from mRNA of CD34-depleted cord blood cells. The sequence of the cDNA contained an open reading frame encoding a putative 19-residue signal peptide and a mature protein of 126 amino acids with two disulfide bridges and enzyme-binding motifs homologous to those of Family 2 cystatins. Unlike other human cystatins, cystatin F has 2 additional Cys residues, indicating the presence of an extra disulfide bridge stabilizing the N-terminal region of the molecule. Recombinant cystatin F was produced in a baculovirus expression system and characterized. The mature recombinant protein processed by insect cells had an N-terminal segment 7 residues longer than that of cystatin C and displayed reversible inhibition of papain and cathepsin L (Ki = 1.1 and 0.31 nM, respectively), but not cathepsin B. Like cystatin E/M, cystatin F is a glycoprotein, carrying two N-linked carbohydrate chains at positions 36 and 88. An immunoassay for quantification of cystatin F showed that blood contains low levels of the inhibitor (0.9 ng/ml). Six B cell lines in culture secreted barely detectable amounts of cystatin F, but several T cell lines and especially one myeloid cell line secreted significant amounts of the inhibitor. Northern blot analysis revealed that the cystatin F gene is primarily expressed in peripheral blood cells and spleen. Tissue expression clearly different from that of the ubiquitous inhibitor, cystatin C, was also indicated by a high incidence of cystatin F clones in cDNA libraries from dendritic and T cells, but no clones identified by expressed sequence tag sequencing in several B cell libraries and in >600 libraries from other human tissues and cells.
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| 2. |
- Dalman, C, et al.
(författare)
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Signs of asphyxia at birth and risk of schizophrenia. Population-based case-control study
- 2001
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Ingår i: The British journal of psychiatry : the journal of mental science. - : Royal College of Psychiatrists. - 0007-1250. ; 179, s. 403-408
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Tidskriftsartikel (refereegranskat)abstract
- Previous research has found an association between obstetric complications and schizophrenia, but in many studies the sample size was limited, and no assessment of specific exposures was possible.AimsTo assess the role of different complications, and in particular to distinguish between disordered foetal development and hypoxia at birth.MethodFrom the Stockholm County In-Patient Register and community registers, we identified 524 cases of schizophrenia and 1043 controls, matched for age, gender, hospital and parish of birth. Data on obstetric complications were obtained from birth records.ResultsThere was a strong association between signs of asphyxia at birth and schizophrenia (OR 4.4; 95% C11.9–10.3) after adjustment for other obstetric complications, maternal history of psychotic illness and social class.ConclusionsSigns of asphyxia at birth are associated with an increased risk of schizophrenia in adults.
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| 3. |
- Lewis, G, et al.
(författare)
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Asphyxia at birth and schizophrenia
- 2002
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Ingår i: The British journal of psychiatry : the journal of mental science. - : Royal College of Psychiatrists. - 0007-1250. ; 180, s. 465-465
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 4. |
- Thomas, HV, et al.
(författare)
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Obstetric complications and risk of schizophrenia. Effect of gender, age at diagnosis and maternal history of psychosis
- 2001
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Ingår i: The British journal of psychiatry : the journal of mental science. - : Royal College of Psychiatrists. - 0007-1250. ; 179, s. 409-414
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Tidskriftsartikel (refereegranskat)abstract
- Obstetric complications have been studied frequently as possible risk factors for schizophrenia.AimsTo test the hypotheses that individual obstetric complications are most strongly associated with an increased risk of schizophrenia in males, in patients with an early age at first diagnosis and in subjects with a maternal history of psychosis.MethodCases of schizophrenia diagnosed between January 1971 and June 1994 were identified in the Stockholm County In-Patient Register. Controls were matched on age, gender, hospital of birth and parish of birth. Obstetric data were recorded blind to case–control status for 524 cases and 1043 controls.ResultsThis study did not find any large or consistent effect of gender, age at diagnosis or maternal history of psychosis on the risk of schizophrenia associated with individual complications.ConclusionsFuture studies should examine these effects using a much larger sample that includes patients with schizophrenia and control subjects whose genetic risk of schizophrenia has been assessed accurately.
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