| 1. |
- Nayak, Tanmaya, et al.
(författare)
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Synergistic degradation of Chlorpyrifos by modified solar Photo-Fenton process with bacterial metabolism reduces in vivo biotoxicity in zebrafish (Danio rerio)
- 2023
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Ingår i: Science of the Total Environment. - : Elsevier BV. - 0048-9697 .- 1879-1026. ; 891
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Tidskriftsartikel (refereegranskat)abstract
- The extensive use of Chlorpyrifos (CP) as insecticide has raised concern to their hazardous impact on human health and ecosystems. Bioremediation has been proved as one of the key eco-compatible method for reducing these environ-mental toxicants. This study explores and evaluate the effectiveness of a combined process including solar Photo-Fenton process followed by bacterial degradation using Ochrobactrum sp. CPD-03 for effective CP degradation in wastewater. Moreover, the in vivo molecular biotoxicity of CP and degraded CP has been evaluated with embryonic zebrafish. The solar Photo-Fenton treatment showed CP degradation efficiency of-42 % in 4 h and-92 % in 96 h with combined bacterial degradation process. In vivo biotoxicity analysis showed increased survivability of embryonic zebrafish exposed to CP with CPD-03 in water with lesser morphological abnormalities. The mechanistic molecular analysis showed decreased acetylcholinesterase inhibition and GST activity in embryos exposed to CP with CPD-03 for a lesser apoptosis due to influential intrinsic interaction with metabolic proteins. The study advocated to the use of solar Photo-Fenton process followed by bacterial degradation for an efficient ecological degradation of CP for effec-tive reduction of in vivo biotoxicity.
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| 2. |
- Choudhury, Anmol, et al.
(författare)
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Atmospheric microplastic and nanoplastic : The toxicological paradigm on the cellular system
- 2023
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Ingår i: Ecotoxicology and Environmental Safety. - : Elsevier BV. - 0147-6513 .- 1090-2414. ; 259
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Tidskriftsartikel (refereegranskat)abstract
- The increasing demand for plastic in our daily lives has led to global plastic pollution. The improper disposal of plastic has resulted in a massive amount of atmospheric microplastics (MPs), which has further resulted in the production of atmospheric nanoplastics (NPs). Because of its intimate relationship with the environment and human health, microplastic and nanoplastic contamination is becoming a problem. Because microplastics and nanoplastics are microscopic and light, they may penetrate deep into the human lungs. Despite several studies demonstrating the abundance of microplastics and nanoplastics in the air, the potential risks of atmospheric microplastics and nanoplastics remain unknown. Because of its small size, atmospheric nanoplastic character-ization has presented significant challenges. This paper describes sampling and characterization procedures for atmospheric microplastics and nanoplastics. This study also examines the numerous harmful effects of plastic particles on human health and other species. There is a significant void in research on the toxicity of airborne microplastics and nanoplastics upon inhalation, which has significant toxicological potential in the future. Further study is needed to determine the influence of microplastic and nanoplastic on pulmonary diseases.
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| 3. |
- Ghosh, Aishee, et al.
(författare)
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Proximal discrepancies in intrinsic atomic interaction determines comparative in vivo biotoxicity of Chlorpyrifos and 3,5,6-trichloro-2-pyridinol in embryonic zebrafish
- 2024
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Ingår i: Science of the Total Environment. - : Elsevier. - 0048-9697 .- 1879-1026. ; 913
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Tidskriftsartikel (refereegranskat)abstract
- Bioaccumulation of Chlorpyrifos (CP) as pesticides due to their aggrandized use in agriculture has raised serious concern on the health of ecosystem and human beings. Moreover, their degraded products like 3,5,6-trichloro-2pyridinol (TCP) has enhanced the distress due to their unpredictable biotoxicity. This study evaluates and deduce the comparative in vivo mechanistic biotoxicity of CP and TCP with zebrafish embryos through experimental and computational approach. Experimental cellular and molecular analysis showed higher induction of morphological abnormalities, oxidative stress and apoptosis in TCP exposed embryos compared to CP exposure due to upregulation of metabolic enzymes like Zhe1a, Sod1 and p53. Computational analysis excavated the differential discrepancies in intrinsic atomic interaction as a reason of disparity in biotoxicity of CP and TCP. The mechanistic differences were deduced due to the differential accumulation and internalisation leading to variable interaction with metabolic enzymes for oxidative stress and apoptosis causing physiological and morphological abnormalities. The study unravelled the information of in vivo toxicity at cellular and molecular level to advocate the attention of taking measures for management of CP as well as TCP for environmental and human health.
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| 4. |
- Husain, Shaheen, et al.
(författare)
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Emerging Trends in Advanced Translational Applications of Silver Nanoparticles : A Progressing Dawn of Nanotechnology
- 2023
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Ingår i: Journal of Functional Biomaterials. - : MDPI. - 2079-4983. ; 14:1
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Forskningsöversikt (refereegranskat)abstract
- Nanoscience has emerged as a fascinating field of science, with its implementation in multiple applications in the form of nanotechnology. Nanotechnology has recently been more impactful in diverse sectors such as the pharmaceutical industry, agriculture sector, and food market. The peculiar properties which make nanoparticles as an asset are their large surface area and their size, which ranges between 1 and 100 nanometers (nm). Various technologies, such as chemical and biological processes, are being used to synthesize nanoparticles. The green chemistry route has become extremely popular due to its use in the synthesis of nanoparticles. Nanomaterials are versatile and impactful in different day to day applications, resulting in their increased utilization and distribution in human cells, tissues, and organs. Owing to the deployment of nanoparticles at a high demand, the need to produce nanoparticles has raised concerns regarding environmentally friendly processes. These processes are meant to produce nanomaterials with improved physiochemical properties that can have significant uses in the fields of medicine, physics, and biochemistry. Among a plethora of nanomaterials, silver nanoparticles have emerged as the most investigated and used nanoparticle. Silver nanoparticles (AgNPs) have become vital entities of study due to their distinctive properties which the scientific society aims to investigate the uses of. The current review addresses the modern expansion of AgNP synthesis, characterization, and mechanism, as well as global applications of AgNPs and their limitations.
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| 5. |
- Kumari, Khushbu, et al.
(författare)
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Amelioration of lipopeptide biosurfactants for enhanced antibacterial and biocompatibility through molecular antioxidant property by methoxy and carboxyl moieties
- 2023
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Ingår i: Biomedicine and Pharmacotherapy. - : Elsevier. - 0753-3322 .- 1950-6007. ; 161
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Tidskriftsartikel (refereegranskat)abstract
- Biosurfactants having surface-active biomolecules have been the cynosure in environment research due to their vast application. However, the lack of information about their low-cost production and detailed mechanistic biocompatibility limits the applicability. The study explores techniques for the production and design of lowcost, biodegradable, and non-toxic biosurfactants from Brevibacterium casei strain LS14 and excavates the mechanistic details of their biomedical properties like antibacterial effects and biocompatibility. Taguchi's design of experiment was used to optimize for enhancing biosurfactant production by optimal factor combinations like Waste glycerol (1%v/v), peptone (1%w/v), NaCl 0.4% (w/v), and pH 6. Under optimal conditions, the purified biosurfactant reduced the surface tension to 35 mN/m from 72.8 mN/m (MSM) and a critical micelle concentration of 25 mg/ml was achieved. Spectroscopic analyses of the purified biosurfactant using Nuclear Magnetic Resonance suggested it as a lipopeptide biosurfactant. The evaluation of mechanistic antibacterial, antiradical, antiproliferative, and cellular effects indicated the efficient antibacterial activity (against Pseudomonas aeruginosa) of biosurfactants due to free radical scavenging activity and oxidative stress. Moreover, the cellular cytotoxicity was estimated by MTT and other cellular assays revealing the phenomenon as the dosedependent induction of apoptosis due to free radical scavenging with an LC50 of 55.6 +/- 2.3 mg/ml.
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| 6. |
- Kumari, Khushbu, et al.
(författare)
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Biosurfactant-functionalized Silver nanoparticles infer intrinsic proximal interaction via Lysine and glutamic acid for reduced in vivo molecular biotoxicity with embryonic zebrafish through oxidative stress and apoptosis
- 2023
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Ingår i: Journal of Environmental Chemical Engineering. - : Elsevier BV. - 2213-3437. ; 11:3
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Tidskriftsartikel (refereegranskat)abstract
- The surge of silver nanoparticles (AgNPs) utilization in daily products has raised the concern over their biotoxicity. The concerned biomedical and environmental biotoxicity has raised the quest of biomolecules for the synthesis of AgNPs with better biocompatibility. The emergence of biogenic biosurfactants has sought attention to solve the limitation of synthesizing controlled, stable and biocompatible nanoparticles; owing to their peculiar property of amphiphilic nature. This study provides a novel approach for functionalizing the silver nanoparticles (AgNPs) using lipopeptide biosurfactant extracted from Brevibacterium casei LS14 for higher in vivo environmental biocompatibility. Microbial surfactant was extracted, purified, and characterized using nuclear magnetic resonance (NMR) showing the presence of chemical moieties like carboxyl, methoxy, and amide. Successful functionalization of AgNP termed "F-AgNP" was done to produce AgNPs with a size of 45.0 & PLUSMN; 2.1 nm. The optical characterization of F-AgNP showed an SPR peak at 404 nm in UV-Visible spectra and zeta potential of - 25.5 & PLUSMN; 8.5 mV. In vivo molecular cytotoxicity analysis with embryonic zebrafish determined an LC50 of 50.2 & mu;g/ml for F-AgNP compared to 33.6 & mu;g/ml of unfunctionalized AgNP (U-AgNP). The mechanistic evaluation depicted the concentration-dependent higher cellular and molecular biocompatibility of F-AgNP compared to U-AgNP with less ROS and apoptosis induction due internalization and interaction of F-AgNP with different amino acids of metabolic proteins like Sod1 and P53 proteins via hydrogen bonds having a variable bond-length to influence their expression. The study delineated the molecular mechanism and suggested a sustainable approach to functionalize AgNP using biosurfactants with for biomedical and environmental applications.
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| 7. |
- Kumari, Khushbu, et al.
(författare)
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The paradigm of prophylactic viral outbreaks measures by microbial biosurfactants
- 2023
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Ingår i: Journal of Infection and Public Health. - : Elsevier. - 1876-0341 .- 1876-035X. ; 16:4, s. 575-587
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Forskningsöversikt (refereegranskat)abstract
- The recent emergence and outbreak of the COVID-19 pandemic confirmed the incompetence of countries across the world to deal with a global public health emergency. Although the recent advent of vaccines is an important prophylactic measure, effective clinical therapy for SARS-Cov-2 is yet to be discovered. With the increasing mortality rate, research has been focused on understanding the pathogenic mechanism and clinical parameters to comprehend COVID-19 infection and propose new avenues for naturally occurring molecules with novel therapeutic properties to alleviate the current situation. In accordance with recent clinical studies and SARS-CoV-2 infection markers, cytokine storm and oxidative stress are entwined pathogenic processes in COVID-19 progression. Lately, Biosurfactants (BSs) have been studied as one of the most advanced biomolecules of microbial origin with anti-inflammatory, antioxidant, antiviral properties, antiadhesive, and antimicrobial properties. Therefore, this review inspects available literature and proposes biosurfactants with these properties to be encouraged for their extensive study in dealing with the current pandemic as new pharmaceutics in the prevention and control of viral spread, treating the symptoms developed after the incubation period through different therapeutic approaches and playing a potential drug delivery model.
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| 8. |
- Verma, Suresh K., et al.
(författare)
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The posterity of Zebrafish in paradigm of in vivo molecular toxicological profiling
- 2024
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Ingår i: Biomedicine and Pharmacotherapy. - : Elsevier. - 0753-3322 .- 1950-6007. ; 171
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Forskningsöversikt (refereegranskat)abstract
- The aggrandised advancement in utility of advanced day-to-day materials and nanomaterials has raised serious concern on their biocompatibility with human and other biotic members. In last few decades, understanding of toxicity of these materials has been given the centre stage of research using many in vitro and in vivo models. Zebrafish (Danio rerio), a freshwater fish and a member of the minnow family has garnered much attention due to its distinct features, which make it an important and frequently used animal model in various fields of embryology and toxicological studies. Given that fertilization and development of zebrafish eggs take place externally, they serve as an excellent model organism for studying early developmental stages. Moreover, zebrafish possess a comparable genetic composition to humans and share almost 70% of their genes with mammals. This particular model organism has become increasingly popular, especially for developmental research. Moreover, it serves as a link between in vitro studies and in vivo analysis in mammals. It is an appealing choice for vertebrate research, when employing high-throughput methods, due to their small size, swift development, and relatively affordable laboratory setup. This small vertebrate has enhanced comprehension of pathobiology and drug toxicity. This review emphasizes on the recent developments in toxicity screening and assays, and the new insights gained about the toxicity of drugs through these assays. Specifically, the cardio, neural, and, hepatic toxicology studies inferred by applications of nanoparticles have been highlighted.
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