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- Das, Nandan, 1983-, et al.
(author)
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Characterization of nanosensitive multifractality in submicron scale tissue morphology and its alteration in tumor progression
- 2021
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In: Journal of Biomedical Optics. - : SPIE - International Society for Optical Engineering. - 1083-3668 .- 1560-2281. ; 26:1
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Journal article (peer-reviewed)abstract
- Significance: Assessment of disease using optical coherence tomography is an actively investigated problem, owing to many unresolved challenges in early disease detection, diagnosis, and treatment response monitoring. The early manifestation of disease or precancer is typically associated with subtle alterations in the tissue dielectric and ultrastructural morphology. In addition, biological tissue is known to have ultrastructural multifractality.Aim: Detection and characterization of nanosensitive structural morphology and multifractality in the tissue submicron structure. Quantification of nanosensitive multifractality and its alteration in progression of tumor.Approach: We have developed a label free nanosensitive multifractal detrended fluctuation analysis(nsMFDFA) technique in combination with multifractal analysis and nanosensitive optical coherence tomography (nsOCT). The proposed method deployed for extraction and quantification of nanosensitive multifractal parameters in mammary fat pad (MFP).Results: Initially, the nsOCT approach is numerically validated on synthetic submicron axial structures. The nsOCT technique was applied to pathologically characterized MFP of murine breast tissue to extract depth-resolved nanosensitive submicron structures. Subsequently, two-dimensional MFDFA were deployed on submicron structural en face images to extract nanosensitive tissue multifractality. We found that nanosensitive multifractality increases in transition from healthy to tumor.Conclusions: This method for extraction of nanosensitive tissue multifractality promises to provide a noninvasive diagnostic tool for early disease detection and monitoring treatment response. The novel ability to delineate the dominant submicron scale nanosensitive multifractal properties may also prove useful for characterizing a wide variety of complex scattering media of non-biological origin.
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| 3. |
- Das, Nandan, 1983-, et al.
(author)
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Nanoscale structure detection and monitoring of tumour growth with optical coherence tomography
- 2020
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In: Nanoscale Advances. - : ROYAL SOC CHEMISTRY. - 2516-0230. ; 2:7, s. 2853-2858
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Journal article (peer-reviewed)abstract
- Approximately 90% of cancers originate in epithelial tissues leading to epithelial thickening, but the ultrastructural changes and underlying architecture are less well known. Depth resolved label free visualization of nanoscale tissue morphology is required to reveal the extent and distribution of ultrastructural changes in underlying tissue, but is difficult to achieve with existing imaging modalities. We developed a nanosensitive optical coherence tomography (nsOCT) approach to provide suchimaging based on dominant axial structure with a few nanometre detection accuracy. nsOCT maps the distribution of axial structural sizes an order of magnitude smaller than the axial resolution of the system. We validated nsOCT methodology by detecting synthetic axial structure via numerical simulations. Subsequently, we validated the nsOCT technique experimentally by detecting known structures from a commercially fabricated sample. nsOCT reveals scaling with different depth of dominant submicronstructural changes associated with carcinoma which may inform the origins of the disease, its progression and improve diagnosis.
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