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- Andersson, Ann-Sofie, et al.
(författare)
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Cell adhesion on supported lipid bilayers
- 2003
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Ingår i: Journal of biomedical materials research. Part A. - : Wiley. - 1549-3296 .- 1552-4965. ; 64:4, s. 622-9
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Tidskriftsartikel (refereegranskat)abstract
- The cell and protein repellent properties of supported phospholipid bilayer (SPB) membranes were investigated. The SPBs were prepared by vesicle adsorption on SiO(2) surfaces. The vesicles of phosphatidylcholine fuse and rupture, and form a supported bilayer covering the surface. We carried out cell culture experiments on several surfaces, including SPBs, using two types of epithelial cells to address the cell adhesional properties. The Quartz Crystal Microbalance Dissipation (QCM-D) technique was used to monitor the SPB formation and subsequent protein adsorption. Neither cell type adhered or proliferated on SiO(2) surfaces coated with SPBs, whereas both cell types adhered and proliferated on the three control surfaces of SiO(2), tissue culture glass, and TiO(2). The QCM-D measurements showed that about two orders of magnitude less mass adsorbed on a SPB surface compared to a TiO(2) surface, from serum-containing media (10% fetal bovine serum). The reduced adsorption on the SPB is a likely explanation for the nondetectable epithelial cell adhesion on the SPB surface. Biomembranes are therefore attractive candidate systems to achieve alternating cell-resistant and cell-interacting regions on surfaces, by including specific cell-binding proteins in the latter regions.
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| 4. |
- Glasmästar, Karin, 1971
(författare)
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Surface Modifications for Biointerfaces - Supported Lipid Bilayers and some Aspects of Microcontact Printing
- 2002
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Biointerfaces engage several disciplines: physics, materials science, chemistry, biology and medicine. They are found on the surfaces of implants, tubes and tanks in the food industry or on the hull of a boat. In this work, model surfaces aimed for contact with biological systems were studied. The overall goal is to use the knowledge gained from this type of basic research in applications of more practical nature. Supported phospholipid bilayers (SPBs) are interesting in this context because they are deposited on solid surfaces where they mimic the biomembranes enclosing living cells. When vesicles of phosphatidyl-choline lipids come in contact with, for example, an SiO2 surface, they first adsorb intact and then transform into an SPB. The Quartz Crystal Microbalance with Dissipation (QCM-D) technique and Surface Plasmon Resonance (SPR) were used to study the kinetics of formation of SPBs. Also the adsorption of proteins (by QCM-D and SPR) and the attachment of cells (by microsocopy) to the SPBs were studied. The experiments revealed very little protein adsorption and no cell attachment. SPBs are thus shown to be quite non-adhesive surfaces, which can be used to produce surfaces with alternating non-adsorbing and adsorbing regions for e.g. biosensors or cell culture. A common technique for micro-patterning of large surface areas is Micro-contact Printing (.my.CP). In this work, the (undesired) transfer of stamp material to the surface during .my.CP was measured and characterised using mainly X-ray photo-electron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The material transfer to the substrate surface was found to be significantly reduced when the stamp was oxidised by UV/ozone treatment before stamping.
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