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Numrering	Referens	Omslagsbild	Hitta
1.	Paloschi, V, et al. (författare) Utilization of an Artery-on-a-Chip to Unravel Novel Regulators and Therapeutic Targets in Vascular Diseases 2024 Ingår i: Advanced healthcare materials. - 2192-2659. ; 13:6, s. e2302907- Tidskriftsartikel (refereegranskat)
2.	Fasolo, F, et al. (författare) The circular RNA Ataxia Telangiectasia Mutated regulates oxidative stress in smooth muscle cells in expanding abdominal aortic aneurysms 2023 Ingår i: Molecular therapy. Nucleic acids. - 2162-2531. ; 33, s. 848-865 Tidskriftsartikel (refereegranskat)
3.	Fasolo, F, et al. (författare) The circular RNA Ataxia Telangiectasia Mutated regulates oxidative stress in smooth muscle cells in expanding abdominal aortic aneurysms 2023 Ingår i: Molecular therapy. Nucleic acids. - 2162-2531. ; 33, s. 848-865 Tidskriftsartikel (refereegranskat)
4.	Fasolo, F, et al. (författare) Long Noncoding RNA MIAT Controls Advanced Atherosclerotic Lesion Formation and Plaque Destabilization 2021 Ingår i: Circulation. - 1524-4539. ; 144:19, s. 1567-1583 Tidskriftsartikel (refereegranskat)
5.	Pelisek, J, et al. (författare) Biobanking: Objectives, Requirements, and Future Challenges-Experiences from the Munich Vascular Biobank 2019 Ingår i: Journal of clinical medicine. - : MDPI AG. - 2077-0383. ; 8:2 Tidskriftsartikel (refereegranskat)abstract Collecting biological tissue samples in a biobank grants a unique opportunity to validate diagnostic and therapeutic strategies for translational and clinical research. In the present work, we provide our long-standing experience in establishing and maintaining a biobank of vascular tissue samples, including the evaluation of tissue quality, especially in formalin-fixed paraffin-embedded specimens (FFPE). Our Munich Vascular Biobank includes, thus far, vascular biomaterial from patients with high-grade carotid artery stenosis (n = 1567), peripheral arterial disease (n = 703), and abdominal aortic aneurysm (n = 481) from our Department of Vascular and Endovascular Surgery (January 2004–December 2018). Vascular tissue samples are continuously processed and characterized to assess tissue morphology, histological quality, cellular composition, inflammation, calcification, neovascularization, and the content of elastin and collagen fibers. Atherosclerotic plaques are further classified in accordance with the American Heart Association (AHA), and plaque stability is determined. In order to assess the quality of RNA from FFPE tissue samples over time (2009–2018), RNA integrity number (RIN) and the extent of RNA fragmentation were evaluated. Expression analysis was performed with two housekeeping genes—glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-actin (ACTB)—using TaqMan-based quantitative reverse-transcription polymerase chain reaction (qRT)-PCR. FFPE biospecimens demonstrated unaltered RNA stability over time for up to 10 years. Furthermore, we provide a protocol for processing tissue samples in our Munich Vascular Biobank. In this work, we demonstrate that biobanking is an important tool not only for scientific research but also for clinical usage and personalized medicine.

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Resultat 1-5 av 5

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Typ av publikation: tidskriftsartikel (5)

Typ av innehåll: refereegranskat (5)

Författare/redaktör: Maegdefessel, L (5); Pauli, J (5); Glukha, N (5); Eckstein, HH (4); Busch, A (4); Paloschi, V (4); visa fler...; Winski, G (4); Fasolo, F (3); Sachs, N (3); Boon, RA (3); Winter, H (3); Knappich, C (3); Wu, ZY (3); Jin, H. (2); Roy, J. (2); Hultgren, R. (2); Chernogubova, E (2); Bauer, S (2); Pelisek, J (2); Metschl, S (2); Ritzer, J (2); Li, ZL (2); Wu, Z. (1); Zimmermann, A. (1); Li, Z (1); Soehnlein, O (1); Reilly, M (1); Hedin, U (1); Li, DY (1); Kempf, W (1); Matic, L (1); Backlund, A (1); Bergmark, C (1); Eckstein, H. H. (1); Reeps, C (1); Trenner, M (1); Schaeffer, C (1); Tsantilas, P (1); Schmid, S. (1); Dueck, A (1); Koschinsky, ML (1); Hegenloh, R (1); Kallmayer, M. (1); Menges, AL (1); Reutersberg, B (1); Botti, L (1); Meucci, S (1); Conti, P (1); Rogowitz, F (1); Hummel, N (1); visa färre...

Lärosäte: Karolinska Institutet (5)

Språk: Engelska (5)

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