| 1. |
- Moens, T. G., et al.
(författare)
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Sense and antisense RNA are not toxic in Drosophila models of C9orf72-associated ALS/FTD
- 2018
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Ingår i: Acta Neuropathologica. - : Springer Science and Business Media LLC. - 0001-6322 .- 1432-0533. ; 135:3, s. 445-457
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Tidskriftsartikel (refereegranskat)abstract
- A GGGGCC hexanucleotide repeat expansion in the C9orf72 gene is the most common genetic cause of amyotrophic lateral sclerosis and frontotemporal dementia. Neurodegeneration may occur via transcription of the repeats into inherently toxic repetitive sense and antisense RNA species, or via repeat-associated non-ATG initiated translation (RANT) of sense and antisense RNA into toxic dipeptide repeat proteins. We have previously demonstrated that regular interspersion of repeat RNA with stop codons prevents RANT (RNA-only models), allowing us to study the role of repeat RNA in isolation. Here we have created novel RNA-only Drosophila models, including the first models of antisense repeat toxicity, and flies expressing extremely large repeats, within the range observed in patients. We generated flies expressing similar to 100 repeat sense or antisense RNA either as part of a processed polyadenylated transcript or intronic sequence. We additionally created Drosophila expressing >1000 RNA-only repeats in the sense direction. When expressed in adult Drosophila neurons polyadenylated repeat RNA is largely cytoplasmic in localisation, whilst intronic repeat RNA forms intranuclear RNA foci, as does >1000 repeat RNA, thus allowing us to investigate both nuclear and cytoplasmic RNA toxicity. We confirmed that these RNA foci are capable of sequestering endogenous Drosophila RNA-binding proteins, and that the production of dipeptide proteins (poly-glycine-proline, and poly-glycine-arginine) is suppressed in our models. We find that neither cytoplasmic nor nuclear sense or antisense RNA are toxic when expressed in adult Drosophila neurons, suggesting they have a limited role in disease pathogenesis.
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| 2. |
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| 3. |
- Bridge, Joanna S., et al.
(författare)
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The Ly alpha Reference Sample. VIII. Characterizing Ly alpha Scattering in Nearby Galaxies
- 2018
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Ingår i: Astrophysical Journal. - : American Astronomical Society. - 0004-637X .- 1538-4357. ; 852:1
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Tidskriftsartikel (refereegranskat)abstract
- We examine the dust geometry and Ly alpha scattering in the galaxies of the Lyman Alpha Reference Sample (LARS), a set of 14 nearby (0.02 < z < 0.2) Ly alpha-emitting and starbursting systems with Hubble Space Telescope Ly alpha, H alpha, and H beta imaging. We find that the global dust properties determined by line ratios are consistent with other studies, with some of the LARS galaxies exhibiting clumpy dust media, while others of them show significantly lower Ly alpha emission compared to their Balmer decrement. With the LARS imaging, we present Ly alpha/H alpha and H alpha/H beta maps with spatial resolutions as low as similar to 40 pc, and use these data to show that in most galaxies, the dust geometry is best modeled by three distinct regions: a central core where dust acts as a screen, an annulus where dust is distributed in clumps, and an outer envelope where Ly alpha photons only scatter. We show that the dust that affects the escape of Ly alpha is more restricted to the galaxies' central regions, while the larger Ly alpha halos are generated by scattering at large radii. We present an empirical modeling technique to quantify how much Ly alpha scatters in the halo, and find that this characteristic scattering distance correlates with the measured size of the Ly alpha halo. We note that there exists a slight anti-correlation between the scattering distance of Ly alpha and global dust properties.
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| 4. |
- Hu, Weida, et al.
(författare)
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CLASSY VII Lyα Profiles : The Structure and Kinematics of Neutral Gas and Implications for LyC Escape in Reionization-era Analogs
- 2023
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Ingår i: Astrophysical Journal. - 0004-637X .- 1538-4357. ; 956:1
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Tidskriftsartikel (refereegranskat)abstract
- Lyα line profiles are a powerful probe of interstellar medium (ISM) structure, outflow speed, and Lyman-continuum escape fraction. In this paper, we present the Lyα line profiles of the Cosmic Origins Spectrograph (COS) Legacy Archive Spectroscopic SurveY, a sample rich in spectroscopic analogs of reionization-era galaxies. A large fraction of the spectra show a complex profile, consisting of a double-peaked Lyα emission profile in the bottom of a damped, Lyα absorption trough. Such profiles reveal an inhomogeneous ISM. We successfully fit the damped Lyα absorption and the Lyα emission profiles separately, but with complementary covering factors, a surprising result because this approach requires no Lyα exchange between high-NH i and low-NH i paths. The combined distribution of column densities is qualitatively similar to the bimodal distributions observed in numerical simulations. We find an inverse relation between Lyα peak separation and the [O iii]/[O ii] flux ratio, confirming that the covering fraction of Lyman-continuum-thin sightlines increases as the Lyα peak separation decreases. We combine measurements of Lyα peak separation and Lyα red peak asymmetry in a diagnostic diagram, which identifies six Lyman-continuum leakers in the COS Legacy Archive Spectrocopy SurveY (CLASSY) sample. We find a strong correlation between the Lyα trough velocity and the outflow velocity measured from interstellar absorption lines. We argue that greater vignetting of the blueshifted Lyα peak, relative to the redshifted peak, is the source of the well-known discrepancy between shell-model parameters and directly measured outflow properties. The CLASSY sample illustrates how scattering of Lyα photons outside the spectroscopic aperture reshapes Lyα profiles because the distances to these compact starbursts span a large range.
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| 6. |
- Vanzella, E., et al.
(författare)
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An extremely metal-poor star complex in the reionization era : Approaching Population III stars with JWST
- 2023
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Ingår i: Astronomy and Astrophysics. - 0004-6361 .- 1432-0746. ; 678
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Tidskriftsartikel (refereegranskat)abstract
- We present JWST/Near Infrared Spectrograph (NIRSpec) integral field spectroscopy (IFS) of a lensed Population III candidate stellar complex (dubbed Lensed And Pristine 1, LAP1), with a lensing-corrected stellar mass of ≲104 M⊙ and an absolute luminosity of MUV > −11.2 (mUV > 35.6), confirmed at redshift 6.639 ± 0.004. The system is strongly amplified (μ ≳ 100) by straddling a critical line of the Hubble Frontier Field galaxy cluster MACS J0416. Although the stellar continuum is currently not detected in the Hubble and JWST/Near Infrared Camera (NIRCam) and Near Infrared Imager and Slitless Spectrograph (NIRISS) imaging, arclet-like shapes of Lyman and Balmer lines, Lyα, Hγ, Hβ and Hα are detected with NIRSpec IFS with signal-to-noise ratios (S/N) of approximately 5 − 13 and large equivalent widths (> 300 − 2000 Å), along with a remarkably weak [O III]λλ4959, 5007 at S/N ≃ 4. LAP1 shows a large ionizing photon production efficiency, log(ξion[erg Hz−1]) > 26. From the metallicity indexes R23 = ([O III] + [O II])/Hβ ≲ 0.74 and R3 = ([O III]/Hβ) = 0.55 ± 0.14, we derive an oxygen abundance of 12 + log(O/H)≲6.3. Intriguingly, the Hα emission is also measured in mirrored subcomponents where no [O III] is detected, providing even more stringent upper limits on the metallicity if in situ star formation is ongoing in this region (12 + log(O/H) < 6). The formal stellar mass limit of the subcomponents would correspond to ∼103 M⊙ or MUV fainter than −10. Alternatively, this metal-free, pure line-emitting region could be the first case of a fluorescing H I gas region induced by transverse escaping ionizing radiation from a nearby star complex. The presence of large equivalent-width hydrogen lines and the deficiency of metal lines in such a small region make LAP1 the most metal-poor star-forming region currently known in the reionization era and a promising site that may host isolated, pristine stars.
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| 7. |
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| 8. |
- Östlin, Göran, et al.
(författare)
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The Source of Leaking Ionizing Photons from Haro11 : Clues from HST/COS Spectroscopy of Knots A, B, and C
- 2021
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Ingår i: Astrophysical Journal. - : American Astronomical Society. - 0004-637X .- 1538-4357. ; 912:2
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Tidskriftsartikel (refereegranskat)abstract
- Understanding the escape of ionizing (Lyman continuum) photons from galaxies is vital for determining how galaxies contributed to reionization in the early universe. While directly detecting the Lyman continuum from high-redshift galaxies is impossible due to the intergalactic medium, low-redshift galaxies in principle offer this possibility but require observations from space. The first local galaxy for which Lyman continuum escape was found is Haro 11, a luminous blue compact galaxy at z = 0.02, where observations with the FUSE satellite revealed an escape fraction of 3.3%. However, the FUSE aperture covers the entire galaxy, and it is not clear from where the Lyman continuum is leaking out. Here we utilize Hubble Space Telescope/Cosmic Origins Spectrograph spectroscopy in the wavelength range 1100-1700 angstrom of the three knots (A, B, and C) of Haro 11 to study the presence of Ly alpha emission and the properties of intervening gas. We find that all knots have bright Ly alpha emission. UV absorption lines, originating in the neutral interstellar medium, as well as lines probing the ionized medium, are seen extending to blueshifted velocities of 500 km s(-1) in all three knots, demonstrating the presence of an outflowing multiphase medium. We find that knots A and B have large covering fractions of neutral gas, making LyC escape along these sightlines improbable, while knot C has a much lower covering fraction (less than or similar to 50%). Knot C also has the the highest Ly alpha escape fraction, and we conclude that it is the most likely source of the escaping Lyman continuum detected in Haro 11.
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